Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2054 and Pf6N2E2_2055 are separated by 78 nucleotides Pf6N2E2_2055 and Pf6N2E2_2056 overlap by 11 nucleotides Pf6N2E2_2056 and Pf6N2E2_2057 are separated by 66 nucleotides Pf6N2E2_2057 and Pf6N2E2_2058 are separated by 53 nucleotides Pf6N2E2_2058 and Pf6N2E2_2059 are separated by 53 nucleotides
Pf6N2E2_2054 - hypothetical protein, at 2,444,933 to 2,445,274
_2054
Pf6N2E2_2055 - StbE replicon stabilization toxin, at 2,445,353 to 2,445,634
_2055
Pf6N2E2_2056 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 2,445,624 to 2,445,872
_2056
Pf6N2E2_2057 - Phosphatidylserine/phosphatidylglycerophosphate/ cardiolipi n synthases and related enzymes, at 2,445,939 to 2,447,585
_2057
Pf6N2E2_2058 - ChlD component of cobalt chelatase involved in B12 biosynthesis, at 2,447,639 to 2,448,250
_2058
Pf6N2E2_2059 - ChlI component of cobalt chelatase involved in B12 biosynthesis, at 2,448,304 to 2,449,308
_2059
Position (kb)
2445
2446
2447
2448 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2444.946 kb on + strand at 2444.947 kb on - strand at 2444.947 kb on - strand at 2444.947 kb on - strand at 2444.976 kb on + strand, within Pf6N2E2_2054 at 2444.976 kb on + strand, within Pf6N2E2_2054 at 2444.976 kb on + strand, within Pf6N2E2_2054 at 2444.977 kb on - strand, within Pf6N2E2_2054 at 2444.977 kb on - strand, within Pf6N2E2_2054 at 2444.977 kb on - strand, within Pf6N2E2_2054 at 2445.004 kb on - strand, within Pf6N2E2_2054 at 2445.004 kb on - strand, within Pf6N2E2_2054 at 2445.245 kb on + strand at 2445.245 kb on + strand at 2445.246 kb on - strand at 2445.246 kb on - strand at 2445.303 kb on - strand at 2445.303 kb on - strand at 2445.308 kb on - strand at 2445.376 kb on - strand at 2445.434 kb on + strand, within Pf6N2E2_2055 at 2445.435 kb on - strand, within Pf6N2E2_2055 at 2445.435 kb on - strand, within Pf6N2E2_2055 at 2445.435 kb on - strand, within Pf6N2E2_2055 at 2445.435 kb on - strand, within Pf6N2E2_2055 at 2445.482 kb on - strand, within Pf6N2E2_2055 at 2445.627 kb on + strand at 2445.627 kb on + strand at 2445.627 kb on + strand at 2445.628 kb on - strand at 2445.628 kb on - strand at 2445.991 kb on + strand at 2445.994 kb on + strand at 2445.994 kb on + strand at 2445.995 kb on - strand at 2445.995 kb on - strand at 2445.995 kb on - strand at 2445.995 kb on - strand at 2445.995 kb on - strand at 2445.995 kb on - strand at 2446.064 kb on - strand at 2446.075 kb on + strand at 2446.084 kb on + strand at 2446.085 kb on - strand at 2446.085 kb on - strand at 2446.085 kb on - strand at 2446.085 kb on - strand at 2446.147 kb on + strand, within Pf6N2E2_2057 at 2446.147 kb on + strand, within Pf6N2E2_2057 at 2446.148 kb on - strand, within Pf6N2E2_2057 at 2446.733 kb on + strand, within Pf6N2E2_2057 at 2446.733 kb on + strand, within Pf6N2E2_2057 at 2446.910 kb on - strand, within Pf6N2E2_2057 at 2446.979 kb on - strand, within Pf6N2E2_2057 at 2446.979 kb on - strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.984 kb on + strand, within Pf6N2E2_2057 at 2446.985 kb on - strand, within Pf6N2E2_2057 at 2446.985 kb on - strand, within Pf6N2E2_2057 at 2446.985 kb on - strand, within Pf6N2E2_2057 at 2446.985 kb on - strand, within Pf6N2E2_2057 at 2447.187 kb on + strand at 2447.188 kb on - strand, within Pf6N2E2_2057 at 2447.188 kb on - strand, within Pf6N2E2_2057 at 2447.188 kb on - strand, within Pf6N2E2_2057 at 2447.275 kb on + strand, within Pf6N2E2_2057 at 2447.275 kb on + strand, within Pf6N2E2_2057 at 2447.276 kb on - strand, within Pf6N2E2_2057 at 2447.276 kb on - strand, within Pf6N2E2_2057 at 2447.323 kb on + strand, within Pf6N2E2_2057 at 2447.323 kb on + strand, within Pf6N2E2_2057 at 2447.323 kb on + strand, within Pf6N2E2_2057 at 2447.517 kb on + strand at 2447.517 kb on + strand at 2447.537 kb on - strand at 2447.577 kb on + strand at 2447.616 kb on + strand at 2447.616 kb on + strand at 2447.617 kb on - strand at 2447.617 kb on - strand at 2447.617 kb on - strand at 2447.617 kb on - strand at 2447.635 kb on + strand at 2447.635 kb on + strand at 2447.635 kb on + strand at 2447.636 kb on - strand at 2447.636 kb on - strand at 2447.662 kb on + strand at 2447.663 kb on - strand at 2447.663 kb on - strand at 2447.671 kb on - strand at 2448.306 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,444,946 + -0.2 2,444,947 - +0.4 2,444,947 - -0.5 2,444,947 - +0.1 2,444,976 + Pf6N2E2_2054 0.13 -1.4 2,444,976 + Pf6N2E2_2054 0.13 -0.1 2,444,976 + Pf6N2E2_2054 0.13 -0.7 2,444,977 - Pf6N2E2_2054 0.13 -0.5 2,444,977 - Pf6N2E2_2054 0.13 +0.0 2,444,977 - Pf6N2E2_2054 0.13 -0.4 2,445,004 - Pf6N2E2_2054 0.21 -0.4 2,445,004 - Pf6N2E2_2054 0.21 +0.4 2,445,245 + +0.3 2,445,245 + +1.5 2,445,246 - +0.4 2,445,246 - -1.0 2,445,303 - +0.1 2,445,303 - -0.6 2,445,308 - -0.4 2,445,376 - +0.6 2,445,434 + Pf6N2E2_2055 0.29 +1.1 2,445,435 - Pf6N2E2_2055 0.29 +0.0 2,445,435 - Pf6N2E2_2055 0.29 +0.5 2,445,435 - Pf6N2E2_2055 0.29 -0.0 2,445,435 - Pf6N2E2_2055 0.29 +0.3 2,445,482 - Pf6N2E2_2055 0.46 -0.1 2,445,627 + -0.4 2,445,627 + -0.0 2,445,627 + -0.4 2,445,628 - -1.4 2,445,628 - +0.2 2,445,991 + +2.0 2,445,994 + -0.4 2,445,994 + +1.6 2,445,995 - -0.4 2,445,995 - -0.9 2,445,995 - +0.8 2,445,995 - -1.0 2,445,995 - -0.2 2,445,995 - -1.4 2,446,064 - -0.1 2,446,075 + -1.8 2,446,084 + +0.5 2,446,085 - -1.2 2,446,085 - +0.3 2,446,085 - +0.2 2,446,085 - -1.4 2,446,147 + Pf6N2E2_2057 0.13 -0.4 2,446,147 + Pf6N2E2_2057 0.13 -1.1 2,446,148 - Pf6N2E2_2057 0.13 -0.4 2,446,733 + Pf6N2E2_2057 0.48 -0.0 2,446,733 + Pf6N2E2_2057 0.48 -0.8 2,446,910 - Pf6N2E2_2057 0.59 -0.4 2,446,979 - Pf6N2E2_2057 0.63 +0.4 2,446,979 - Pf6N2E2_2057 0.63 -0.6 2,446,984 + Pf6N2E2_2057 0.63 -0.2 2,446,984 + Pf6N2E2_2057 0.63 +0.1 2,446,984 + Pf6N2E2_2057 0.63 -1.0 2,446,984 + Pf6N2E2_2057 0.63 -0.9 2,446,984 + Pf6N2E2_2057 0.63 +0.3 2,446,984 + Pf6N2E2_2057 0.63 +0.0 2,446,984 + Pf6N2E2_2057 0.63 -0.6 2,446,985 - Pf6N2E2_2057 0.64 -1.2 2,446,985 - Pf6N2E2_2057 0.64 -0.3 2,446,985 - Pf6N2E2_2057 0.64 -0.9 2,446,985 - Pf6N2E2_2057 0.64 +0.1 2,447,187 + +0.6 2,447,188 - Pf6N2E2_2057 0.76 -0.2 2,447,188 - Pf6N2E2_2057 0.76 +0.2 2,447,188 - Pf6N2E2_2057 0.76 -0.2 2,447,275 + Pf6N2E2_2057 0.81 -0.8 2,447,275 + Pf6N2E2_2057 0.81 -0.3 2,447,276 - Pf6N2E2_2057 0.81 +0.3 2,447,276 - Pf6N2E2_2057 0.81 -1.3 2,447,323 + Pf6N2E2_2057 0.84 -0.8 2,447,323 + Pf6N2E2_2057 0.84 +0.1 2,447,323 + Pf6N2E2_2057 0.84 -0.1 2,447,517 + +0.1 2,447,517 + +0.3 2,447,537 - -1.3 2,447,577 + +0.2 2,447,616 + -1.6 2,447,616 + +0.3 2,447,617 - -1.0 2,447,617 - -0.3 2,447,617 - +0.7 2,447,617 - +1.3 2,447,635 + -0.9 2,447,635 + +0.9 2,447,635 + -0.4 2,447,636 - -0.1 2,447,636 - -1.0 2,447,662 + -0.1 2,447,663 - -0.7 2,447,663 - -0.6 2,447,671 - -1.9 2,448,306 - -1.1
Or see this region's nucleotide sequence