Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1461 and Pf6N2E2_1462 are separated by 373 nucleotides Pf6N2E2_1462 and Pf6N2E2_1463 are separated by 93 nucleotides Pf6N2E2_1463 and Pf6N2E2_1464 are separated by 308 nucleotides Pf6N2E2_1464 and Pf6N2E2_1465 are separated by 99 nucleotides
Pf6N2E2_1461 - hypothetical protein, at 1,710,391 to 1,710,672
_1461
Pf6N2E2_1462 - Nicotinamidase/isochorismatase family protein, at 1,711,046 to 1,711,564
_1462
Pf6N2E2_1463 - Isochorismate pyruvate-lyase (EC 4.-.-.-), at 1,711,658 to 1,711,936
_1463
Pf6N2E2_1464 - hypothetical protein, at 1,712,245 to 1,712,763
_1464
Pf6N2E2_1465 - hypothetical protein, at 1,712,863 to 1,714,203
_1465
Position (kb)
1711
1712 Strain fitness (log2 ratio)
-1
0
1 at 1710.662 kb on + strand at 1710.769 kb on + strand at 1710.828 kb on + strand at 1710.828 kb on + strand at 1710.959 kb on - strand at 1710.992 kb on + strand at 1711.195 kb on - strand, within Pf6N2E2_1462 at 1711.195 kb on - strand, within Pf6N2E2_1462 at 1711.195 kb on - strand, within Pf6N2E2_1462 at 1711.383 kb on - strand, within Pf6N2E2_1462 at 1711.387 kb on + strand, within Pf6N2E2_1462 at 1711.453 kb on + strand, within Pf6N2E2_1462 at 1711.453 kb on + strand, within Pf6N2E2_1462 at 1711.453 kb on + strand, within Pf6N2E2_1462 at 1711.454 kb on - strand, within Pf6N2E2_1462 at 1711.454 kb on - strand, within Pf6N2E2_1462 at 1711.730 kb on + strand, within Pf6N2E2_1463 at 1711.730 kb on + strand, within Pf6N2E2_1463 at 1711.730 kb on + strand, within Pf6N2E2_1463 at 1711.731 kb on - strand, within Pf6N2E2_1463 at 1711.731 kb on - strand, within Pf6N2E2_1463 at 1712.225 kb on + strand at 1712.226 kb on - strand at 1712.265 kb on + strand at 1712.266 kb on - strand at 1712.266 kb on - strand at 1712.266 kb on - strand at 1712.527 kb on + strand, within Pf6N2E2_1464 at 1712.632 kb on + strand, within Pf6N2E2_1464 at 1712.632 kb on + strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.677 kb on + strand, within Pf6N2E2_1464 at 1712.746 kb on + strand at 1712.746 kb on + strand at 1712.746 kb on + strand at 1712.747 kb on - strand at 1712.779 kb on + strand at 1712.780 kb on - strand at 1712.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,710,662 + -1.5 1,710,769 + +0.2 1,710,828 + +0.3 1,710,828 + +0.5 1,710,959 - +0.4 1,710,992 + +1.0 1,711,195 - Pf6N2E2_1462 0.29 -0.5 1,711,195 - Pf6N2E2_1462 0.29 +1.0 1,711,195 - Pf6N2E2_1462 0.29 +0.3 1,711,383 - Pf6N2E2_1462 0.65 +0.0 1,711,387 + Pf6N2E2_1462 0.66 +0.3 1,711,453 + Pf6N2E2_1462 0.78 -0.3 1,711,453 + Pf6N2E2_1462 0.78 -0.8 1,711,453 + Pf6N2E2_1462 0.78 +0.7 1,711,454 - Pf6N2E2_1462 0.79 +0.2 1,711,454 - Pf6N2E2_1462 0.79 +0.1 1,711,730 + Pf6N2E2_1463 0.26 -0.6 1,711,730 + Pf6N2E2_1463 0.26 -0.7 1,711,730 + Pf6N2E2_1463 0.26 -1.2 1,711,731 - Pf6N2E2_1463 0.26 +0.1 1,711,731 - Pf6N2E2_1463 0.26 -0.1 1,712,225 + -1.7 1,712,226 - -0.0 1,712,265 + -0.1 1,712,266 - +0.6 1,712,266 - +0.2 1,712,266 - -0.4 1,712,527 + Pf6N2E2_1464 0.54 +0.4 1,712,632 + Pf6N2E2_1464 0.75 +0.3 1,712,632 + Pf6N2E2_1464 0.75 -0.1 1,712,633 - Pf6N2E2_1464 0.75 -0.4 1,712,633 - Pf6N2E2_1464 0.75 +0.1 1,712,633 - Pf6N2E2_1464 0.75 +0.4 1,712,677 + Pf6N2E2_1464 0.83 +0.5 1,712,746 + -0.2 1,712,746 + -0.4 1,712,746 + -0.1 1,712,747 - +0.6 1,712,779 + -0.1 1,712,780 - +1.0 1,712,780 - +0.1
Or see this region's nucleotide sequence