Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1321 and Pf6N2E2_1322 overlap by 8 nucleotides Pf6N2E2_1322 and Pf6N2E2_1323 overlap by 8 nucleotides Pf6N2E2_1323 and Pf6N2E2_1324 are separated by 171 nucleotides
Pf6N2E2_1321 - MoaF protein precursor, at 1,554,840 to 1,555,661
_1321
Pf6N2E2_1322 - MoaE protein, at 1,555,654 to 1,556,454
_1322
Pf6N2E2_1323 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 1,556,447 to 1,557,214
_1323
Pf6N2E2_1324 - Transcriptional regulator, AraC family, at 1,557,386 to 1,558,348
_1324
Position (kb)
1556
1557
1558 Strain fitness (log2 ratio)
-1
0
1 at 1555.459 kb on - strand, within Pf6N2E2_1321 at 1555.459 kb on - strand, within Pf6N2E2_1321 at 1555.480 kb on - strand, within Pf6N2E2_1321 at 1555.480 kb on - strand, within Pf6N2E2_1321 at 1555.630 kb on + strand at 1555.648 kb on - strand at 1555.669 kb on - strand at 1555.723 kb on + strand at 1555.911 kb on + strand, within Pf6N2E2_1322 at 1555.990 kb on + strand, within Pf6N2E2_1322 at 1555.991 kb on - strand, within Pf6N2E2_1322 at 1555.991 kb on - strand, within Pf6N2E2_1322 at 1556.263 kb on + strand, within Pf6N2E2_1322 at 1556.263 kb on + strand, within Pf6N2E2_1322 at 1556.264 kb on - strand, within Pf6N2E2_1322 at 1556.264 kb on - strand, within Pf6N2E2_1322 at 1556.412 kb on - strand at 1556.412 kb on - strand at 1556.625 kb on + strand, within Pf6N2E2_1323 at 1556.625 kb on + strand, within Pf6N2E2_1323 at 1556.783 kb on - strand, within Pf6N2E2_1323 at 1557.134 kb on + strand, within Pf6N2E2_1323 at 1557.158 kb on + strand at 1557.158 kb on + strand at 1557.159 kb on - strand at 1557.159 kb on - strand at 1557.515 kb on - strand, within Pf6N2E2_1324 at 1557.515 kb on - strand, within Pf6N2E2_1324 at 1557.584 kb on - strand, within Pf6N2E2_1324 at 1557.586 kb on - strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.713 kb on + strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.714 kb on - strand, within Pf6N2E2_1324 at 1557.717 kb on + strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.718 kb on - strand, within Pf6N2E2_1324 at 1557.770 kb on - strand, within Pf6N2E2_1324 at 1558.098 kb on + strand, within Pf6N2E2_1324 at 1558.157 kb on + strand, within Pf6N2E2_1324
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,555,459 - Pf6N2E2_1321 0.75 -0.6 1,555,459 - Pf6N2E2_1321 0.75 +1.0 1,555,480 - Pf6N2E2_1321 0.78 +0.4 1,555,480 - Pf6N2E2_1321 0.78 -0.2 1,555,630 + +0.3 1,555,648 - -0.4 1,555,669 - -0.0 1,555,723 + -1.2 1,555,911 + Pf6N2E2_1322 0.32 -0.2 1,555,990 + Pf6N2E2_1322 0.42 +0.1 1,555,991 - Pf6N2E2_1322 0.42 +0.5 1,555,991 - Pf6N2E2_1322 0.42 +0.6 1,556,263 + Pf6N2E2_1322 0.76 +0.0 1,556,263 + Pf6N2E2_1322 0.76 +0.1 1,556,264 - Pf6N2E2_1322 0.76 +1.5 1,556,264 - Pf6N2E2_1322 0.76 +0.8 1,556,412 - -0.2 1,556,412 - +0.3 1,556,625 + Pf6N2E2_1323 0.23 -0.3 1,556,625 + Pf6N2E2_1323 0.23 -1.5 1,556,783 - Pf6N2E2_1323 0.44 -0.2 1,557,134 + Pf6N2E2_1323 0.89 -0.2 1,557,158 + -0.3 1,557,158 + +0.7 1,557,159 - -0.2 1,557,159 - -0.2 1,557,515 - Pf6N2E2_1324 0.13 -0.3 1,557,515 - Pf6N2E2_1324 0.13 +0.6 1,557,584 - Pf6N2E2_1324 0.21 +0.1 1,557,586 - Pf6N2E2_1324 0.21 +0.3 1,557,713 + Pf6N2E2_1324 0.34 +0.6 1,557,713 + Pf6N2E2_1324 0.34 +0.6 1,557,713 + Pf6N2E2_1324 0.34 +1.1 1,557,713 + Pf6N2E2_1324 0.34 -0.0 1,557,714 - Pf6N2E2_1324 0.34 +0.2 1,557,714 - Pf6N2E2_1324 0.34 +0.4 1,557,714 - Pf6N2E2_1324 0.34 -0.1 1,557,714 - Pf6N2E2_1324 0.34 +0.2 1,557,717 + Pf6N2E2_1324 0.34 -0.3 1,557,718 - Pf6N2E2_1324 0.34 +1.3 1,557,718 - Pf6N2E2_1324 0.34 +0.7 1,557,718 - Pf6N2E2_1324 0.34 +0.2 1,557,718 - Pf6N2E2_1324 0.34 +0.4 1,557,718 - Pf6N2E2_1324 0.34 +0.8 1,557,770 - Pf6N2E2_1324 0.40 +0.7 1,558,098 + Pf6N2E2_1324 0.74 -1.0 1,558,157 + Pf6N2E2_1324 0.80 -0.4
Or see this region's nucleotide sequence