Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1147 and Pf6N2E2_1148 are separated by 231 nucleotides Pf6N2E2_1148 and Pf6N2E2_1149 are separated by 3 nucleotides
Pf6N2E2_1147 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 1,344,426 to 1,345,199
_1147
Pf6N2E2_1148 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,345,431 to 1,346,582
_1148
Pf6N2E2_1149 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4), at 1,346,586 to 1,347,692
_1149
Position (kb)
1345
1346
1347 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1344.433 kb on - strand at 1344.433 kb on - strand at 1344.433 kb on - strand at 1344.660 kb on + strand, within Pf6N2E2_1147 at 1344.661 kb on - strand, within Pf6N2E2_1147 at 1345.031 kb on + strand, within Pf6N2E2_1147 at 1345.032 kb on - strand at 1345.193 kb on + strand at 1345.193 kb on + strand at 1345.193 kb on + strand at 1345.194 kb on - strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.198 kb on - strand at 1345.423 kb on + strand at 1345.423 kb on + strand at 1345.424 kb on - strand at 1345.441 kb on + strand at 1345.476 kb on + strand at 1345.477 kb on - strand at 1345.495 kb on + strand at 1345.495 kb on + strand at 1345.495 kb on + strand at 1345.496 kb on - strand at 1345.496 kb on - strand at 1345.496 kb on - strand at 1345.522 kb on + strand at 1345.525 kb on + strand at 1345.526 kb on - strand at 1345.526 kb on - strand at 1345.789 kb on + strand, within Pf6N2E2_1148 at 1346.012 kb on + strand, within Pf6N2E2_1148 at 1346.392 kb on + strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.396 kb on + strand, within Pf6N2E2_1148 at 1346.418 kb on + strand, within Pf6N2E2_1148 at 1346.703 kb on + strand, within Pf6N2E2_1149 at 1346.703 kb on + strand, within Pf6N2E2_1149 at 1346.704 kb on - strand, within Pf6N2E2_1149 at 1346.704 kb on - strand, within Pf6N2E2_1149 at 1346.862 kb on + strand, within Pf6N2E2_1149 at 1346.863 kb on - strand, within Pf6N2E2_1149 at 1347.038 kb on + strand, within Pf6N2E2_1149 at 1347.038 kb on + strand, within Pf6N2E2_1149 at 1347.121 kb on + strand, within Pf6N2E2_1149 at 1347.121 kb on + strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.166 kb on + strand, within Pf6N2E2_1149 at 1347.166 kb on + strand, within Pf6N2E2_1149 at 1347.167 kb on - strand, within Pf6N2E2_1149 at 1347.167 kb on - strand, within Pf6N2E2_1149 at 1347.202 kb on + strand, within Pf6N2E2_1149 at 1347.436 kb on + strand, within Pf6N2E2_1149 at 1347.437 kb on - strand, within Pf6N2E2_1149
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,344,433 - -0.1 1,344,433 - -0.2 1,344,433 - -0.4 1,344,660 + Pf6N2E2_1147 0.30 -0.4 1,344,661 - Pf6N2E2_1147 0.30 -0.4 1,345,031 + Pf6N2E2_1147 0.78 -0.2 1,345,032 - +0.4 1,345,193 + -0.0 1,345,193 + -0.3 1,345,193 + -0.3 1,345,194 - -2.0 1,345,197 + -0.5 1,345,197 + -0.0 1,345,197 + +4.5 1,345,197 + +0.5 1,345,198 - -0.2 1,345,423 + -0.3 1,345,423 + +0.1 1,345,424 - -1.0 1,345,441 + -0.7 1,345,476 + -0.1 1,345,477 - +0.4 1,345,495 + -0.3 1,345,495 + -0.5 1,345,495 + +1.9 1,345,496 - -0.4 1,345,496 - +0.0 1,345,496 - -0.3 1,345,522 + +0.3 1,345,525 + +0.3 1,345,526 - -0.8 1,345,526 - -0.0 1,345,789 + Pf6N2E2_1148 0.31 -0.1 1,346,012 + Pf6N2E2_1148 0.50 +0.4 1,346,392 + Pf6N2E2_1148 0.83 +0.4 1,346,393 - Pf6N2E2_1148 0.84 -1.1 1,346,393 - Pf6N2E2_1148 0.84 -0.8 1,346,393 - Pf6N2E2_1148 0.84 -0.3 1,346,396 + Pf6N2E2_1148 0.84 -1.2 1,346,418 + Pf6N2E2_1148 0.86 -0.1 1,346,703 + Pf6N2E2_1149 0.11 -0.2 1,346,703 + Pf6N2E2_1149 0.11 +0.1 1,346,704 - Pf6N2E2_1149 0.11 -0.9 1,346,704 - Pf6N2E2_1149 0.11 +0.0 1,346,862 + Pf6N2E2_1149 0.25 -0.6 1,346,863 - Pf6N2E2_1149 0.25 -0.2 1,347,038 + Pf6N2E2_1149 0.41 +1.0 1,347,038 + Pf6N2E2_1149 0.41 -0.4 1,347,121 + Pf6N2E2_1149 0.48 +0.3 1,347,121 + Pf6N2E2_1149 0.48 -0.3 1,347,122 - Pf6N2E2_1149 0.48 +0.3 1,347,122 - Pf6N2E2_1149 0.48 -0.1 1,347,122 - Pf6N2E2_1149 0.48 -0.1 1,347,122 - Pf6N2E2_1149 0.48 -0.2 1,347,122 - Pf6N2E2_1149 0.48 +0.1 1,347,166 + Pf6N2E2_1149 0.52 +0.1 1,347,166 + Pf6N2E2_1149 0.52 -1.7 1,347,167 - Pf6N2E2_1149 0.52 +0.6 1,347,167 - Pf6N2E2_1149 0.52 -0.9 1,347,202 + Pf6N2E2_1149 0.56 -0.8 1,347,436 + Pf6N2E2_1149 0.77 +1.3 1,347,437 - Pf6N2E2_1149 0.77 +0.8
Or see this region's nucleotide sequence