Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1124

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1122 and Pf6N2E2_1123 are separated by 13 nucleotidesPf6N2E2_1123 and Pf6N2E2_1124 overlap by 4 nucleotidesPf6N2E2_1124 and Pf6N2E2_1125 are separated by 142 nucleotides Pf6N2E2_1122 - MotA/TolQ/ExbB proton channel family protein, at 1,318,799 to 1,319,506 _1122 Pf6N2E2_1123 - Tol biopolymer transport system, TolR protein, at 1,319,520 to 1,319,966 _1123 Pf6N2E2_1124 - Ferric siderophore transport system, periplasmic binding protein TonB, at 1,319,963 to 1,320,778 _1124 Pf6N2E2_1125 - Transcriptional regulator, AraC family, at 1,320,921 to 1,321,817 _1125 Position (kb) 1319 1320 1321Strain fitness (log2 ratio) -2 -1 0 1 2at 1319.006 kb on + strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.223 kb on - strandat 1319.381 kb on + strand, within Pf6N2E2_1122at 1319.381 kb on + strand, within Pf6N2E2_1122at 1319.427 kb on - strand, within Pf6N2E2_1122at 1319.427 kb on - strand, within Pf6N2E2_1122at 1319.427 kb on - strand, within Pf6N2E2_1122at 1319.577 kb on + strand, within Pf6N2E2_1123at 1319.577 kb on + strand, within Pf6N2E2_1123at 1319.737 kb on - strand, within Pf6N2E2_1123at 1319.739 kb on + strand, within Pf6N2E2_1123at 1319.739 kb on + strand, within Pf6N2E2_1123at 1319.740 kb on - strand, within Pf6N2E2_1123at 1319.740 kb on - strand, within Pf6N2E2_1123at 1319.740 kb on - strand, within Pf6N2E2_1123at 1319.856 kb on + strand, within Pf6N2E2_1123at 1319.857 kb on - strand, within Pf6N2E2_1123at 1319.857 kb on - strand, within Pf6N2E2_1123at 1320.207 kb on + strand, within Pf6N2E2_1124at 1320.599 kb on + strand, within Pf6N2E2_1124at 1320.600 kb on - strand, within Pf6N2E2_1124at 1320.600 kb on - strand, within Pf6N2E2_1124at 1320.754 kb on + strandat 1320.755 kb on - strandat 1320.789 kb on + strandat 1320.895 kb on - strandat 1320.922 kb on + strandat 1320.922 kb on + strandat 1320.922 kb on + strandat 1320.923 kb on - strandat 1321.081 kb on + strand, within Pf6N2E2_1125at 1321.081 kb on + strand, within Pf6N2E2_1125at 1321.082 kb on - strand, within Pf6N2E2_1125at 1321.082 kb on - strand, within Pf6N2E2_1125at 1321.132 kb on + strand, within Pf6N2E2_1125at 1321.143 kb on - strand, within Pf6N2E2_1125at 1321.241 kb on + strand, within Pf6N2E2_1125at 1321.242 kb on - strand, within Pf6N2E2_1125at 1321.300 kb on - strand, within Pf6N2E2_1125at 1321.484 kb on - strand, within Pf6N2E2_1125at 1321.537 kb on + strand, within Pf6N2E2_1125at 1321.538 kb on - strand, within Pf6N2E2_1125

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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1,319,006 + Pf6N2E2_1122 0.29 +0.1
1,319,170 - Pf6N2E2_1122 0.52 +0.1
1,319,170 - Pf6N2E2_1122 0.52 -0.4
1,319,170 - Pf6N2E2_1122 0.52 +0.8
1,319,170 - Pf6N2E2_1122 0.52 -0.5
1,319,223 - +1.8
1,319,381 + Pf6N2E2_1122 0.82 +0.2
1,319,381 + Pf6N2E2_1122 0.82 -1.9
1,319,427 - Pf6N2E2_1122 0.89 -0.7
1,319,427 - Pf6N2E2_1122 0.89 -0.5
1,319,427 - Pf6N2E2_1122 0.89 -0.8
1,319,577 + Pf6N2E2_1123 0.13 -1.3
1,319,577 + Pf6N2E2_1123 0.13 +0.4
1,319,737 - Pf6N2E2_1123 0.49 +0.6
1,319,739 + Pf6N2E2_1123 0.49 -0.1
1,319,739 + Pf6N2E2_1123 0.49 +0.6
1,319,740 - Pf6N2E2_1123 0.49 +0.4
1,319,740 - Pf6N2E2_1123 0.49 +0.7
1,319,740 - Pf6N2E2_1123 0.49 -0.2
1,319,856 + Pf6N2E2_1123 0.75 +0.2
1,319,857 - Pf6N2E2_1123 0.75 +0.5
1,319,857 - Pf6N2E2_1123 0.75 +0.4
1,320,207 + Pf6N2E2_1124 0.30 -0.9
1,320,599 + Pf6N2E2_1124 0.78 -0.2
1,320,600 - Pf6N2E2_1124 0.78 -0.4
1,320,600 - Pf6N2E2_1124 0.78 -0.3
1,320,754 + -0.5
1,320,755 - +0.5
1,320,789 + -0.4
1,320,895 - -0.6
1,320,922 + -0.6
1,320,922 + +0.2
1,320,922 + -0.4
1,320,923 - -0.8
1,321,081 + Pf6N2E2_1125 0.18 +0.6
1,321,081 + Pf6N2E2_1125 0.18 +0.8
1,321,082 - Pf6N2E2_1125 0.18 +0.4
1,321,082 - Pf6N2E2_1125 0.18 +0.4
1,321,132 + Pf6N2E2_1125 0.24 +0.2
1,321,143 - Pf6N2E2_1125 0.25 -0.0
1,321,241 + Pf6N2E2_1125 0.36 -0.8
1,321,242 - Pf6N2E2_1125 0.36 -0.0
1,321,300 - Pf6N2E2_1125 0.42 +0.1
1,321,484 - Pf6N2E2_1125 0.63 -0.3
1,321,537 + Pf6N2E2_1125 0.69 -0.6
1,321,538 - Pf6N2E2_1125 0.69 +0.3

Or see this region's nucleotide sequence