Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1047 and Pf6N2E2_1048 are separated by 111 nucleotides Pf6N2E2_1048 and Pf6N2E2_1049 are separated by 30 nucleotides
Pf6N2E2_1047 - Bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-); Similar to arginate lyase, at 1,220,988 to 1,221,992
_1047
Pf6N2E2_1048 - Transcriptional regulator, HxlR family, at 1,222,104 to 1,222,562
_1048
Pf6N2E2_1049 - hypothetical protein, at 1,222,593 to 1,222,730
_1049
Position (kb)
1222
1223 Strain fitness (log2 ratio)
-2
-1
0
1 at 1221.733 kb on + strand, within Pf6N2E2_1047 at 1221.967 kb on + strand at 1221.967 kb on + strand at 1221.968 kb on - strand at 1221.979 kb on + strand at 1222.031 kb on + strand at 1222.032 kb on - strand at 1222.042 kb on + strand at 1222.096 kb on + strand at 1222.105 kb on + strand at 1222.294 kb on + strand, within Pf6N2E2_1048 at 1222.295 kb on - strand, within Pf6N2E2_1048 at 1222.295 kb on - strand, within Pf6N2E2_1048 at 1222.295 kb on - strand, within Pf6N2E2_1048 at 1222.320 kb on + strand, within Pf6N2E2_1048 at 1222.320 kb on + strand, within Pf6N2E2_1048 at 1222.320 kb on + strand, within Pf6N2E2_1048 at 1222.321 kb on - strand, within Pf6N2E2_1048 at 1222.321 kb on - strand, within Pf6N2E2_1048 at 1222.321 kb on - strand, within Pf6N2E2_1048 at 1222.321 kb on - strand, within Pf6N2E2_1048 at 1222.321 kb on - strand, within Pf6N2E2_1048 at 1222.321 kb on - strand, within Pf6N2E2_1048 at 1222.324 kb on + strand, within Pf6N2E2_1048 at 1222.324 kb on + strand, within Pf6N2E2_1048 at 1222.324 kb on + strand, within Pf6N2E2_1048 at 1222.324 kb on + strand, within Pf6N2E2_1048 at 1222.324 kb on + strand, within Pf6N2E2_1048 at 1222.324 kb on + strand, within Pf6N2E2_1048 at 1222.324 kb on + strand, within Pf6N2E2_1048 at 1222.324 kb on + strand, within Pf6N2E2_1048 at 1222.324 kb on + strand, within Pf6N2E2_1048 at 1222.324 kb on + strand, within Pf6N2E2_1048 at 1222.325 kb on - strand, within Pf6N2E2_1048 at 1222.325 kb on - strand, within Pf6N2E2_1048 at 1222.325 kb on - strand, within Pf6N2E2_1048 at 1222.325 kb on - strand, within Pf6N2E2_1048 at 1222.325 kb on - strand, within Pf6N2E2_1048 at 1222.325 kb on - strand, within Pf6N2E2_1048 at 1222.325 kb on - strand, within Pf6N2E2_1048 at 1222.325 kb on - strand, within Pf6N2E2_1048 at 1222.325 kb on - strand, within Pf6N2E2_1048 at 1222.325 kb on - strand, within Pf6N2E2_1048 at 1222.581 kb on + strand at 1222.581 kb on + strand at 1222.581 kb on + strand at 1222.581 kb on + strand at 1222.581 kb on + strand at 1222.581 kb on + strand at 1222.581 kb on + strand at 1222.582 kb on - strand at 1222.582 kb on - strand at 1222.594 kb on + strand at 1222.594 kb on + strand at 1222.594 kb on + strand at 1222.595 kb on - strand at 1222.597 kb on - strand at 1222.698 kb on + strand, within Pf6N2E2_1049 at 1222.699 kb on - strand, within Pf6N2E2_1049 at 1222.699 kb on - strand, within Pf6N2E2_1049 at 1222.706 kb on + strand, within Pf6N2E2_1049 at 1222.706 kb on + strand, within Pf6N2E2_1049 at 1222.706 kb on + strand, within Pf6N2E2_1049 at 1222.707 kb on - strand, within Pf6N2E2_1049 at 1222.708 kb on + strand, within Pf6N2E2_1049 at 1222.709 kb on - strand, within Pf6N2E2_1049 at 1222.859 kb on + strand at 1222.860 kb on - strand at 1222.860 kb on - strand at 1222.860 kb on - strand at 1222.889 kb on + strand at 1222.889 kb on + strand at 1222.889 kb on + strand at 1222.890 kb on - strand at 1222.890 kb on - strand at 1222.890 kb on - strand at 1222.890 kb on - strand at 1222.890 kb on - strand at 1222.915 kb on + strand at 1222.915 kb on + strand at 1222.915 kb on + strand at 1222.915 kb on + strand at 1222.916 kb on - strand at 1222.916 kb on - strand at 1222.916 kb on - strand at 1222.922 kb on + strand at 1222.979 kb on + strand at 1223.102 kb on + strand at 1223.156 kb on + strand at 1223.156 kb on + strand at 1223.156 kb on + strand at 1223.292 kb on + strand at 1223.292 kb on + strand at 1223.292 kb on + strand at 1223.292 kb on + strand at 1223.342 kb on + strand at 1223.350 kb on + strand at 1223.350 kb on + strand at 1223.351 kb on - strand at 1223.360 kb on + strand at 1223.360 kb on + strand at 1223.388 kb on + strand at 1223.388 kb on + strand at 1223.388 kb on + strand at 1223.388 kb on + strand at 1223.388 kb on + strand at 1223.388 kb on + strand at 1223.388 kb on + strand at 1223.388 kb on + strand at 1223.392 kb on + strand at 1223.412 kb on + strand at 1223.424 kb on + strand at 1223.529 kb on + strand at 1223.529 kb on + strand at 1223.529 kb on + strand at 1223.539 kb on + strand at 1223.571 kb on + strand at 1223.589 kb on + strand at 1223.589 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,221,733 + Pf6N2E2_1047 0.74 -0.6 1,221,967 + +0.4 1,221,967 + -0.4 1,221,968 - -1.7 1,221,979 + -0.3 1,222,031 + +0.0 1,222,032 - -0.5 1,222,042 + -0.1 1,222,096 + -0.1 1,222,105 + -2.4 1,222,294 + Pf6N2E2_1048 0.41 +0.7 1,222,295 - Pf6N2E2_1048 0.42 -1.4 1,222,295 - Pf6N2E2_1048 0.42 -0.0 1,222,295 - Pf6N2E2_1048 0.42 -0.1 1,222,320 + Pf6N2E2_1048 0.47 +0.2 1,222,320 + Pf6N2E2_1048 0.47 +0.3 1,222,320 + Pf6N2E2_1048 0.47 -0.2 1,222,321 - Pf6N2E2_1048 0.47 +0.5 1,222,321 - Pf6N2E2_1048 0.47 +0.2 1,222,321 - Pf6N2E2_1048 0.47 +0.7 1,222,321 - Pf6N2E2_1048 0.47 -1.4 1,222,321 - Pf6N2E2_1048 0.47 -0.8 1,222,321 - Pf6N2E2_1048 0.47 +0.0 1,222,324 + Pf6N2E2_1048 0.48 +1.0 1,222,324 + Pf6N2E2_1048 0.48 +0.2 1,222,324 + Pf6N2E2_1048 0.48 +1.1 1,222,324 + Pf6N2E2_1048 0.48 -0.5 1,222,324 + Pf6N2E2_1048 0.48 -0.1 1,222,324 + Pf6N2E2_1048 0.48 -0.9 1,222,324 + Pf6N2E2_1048 0.48 -0.0 1,222,324 + Pf6N2E2_1048 0.48 +0.5 1,222,324 + Pf6N2E2_1048 0.48 -0.3 1,222,324 + Pf6N2E2_1048 0.48 +0.5 1,222,325 - Pf6N2E2_1048 0.48 -0.5 1,222,325 - Pf6N2E2_1048 0.48 +1.3 1,222,325 - Pf6N2E2_1048 0.48 -2.0 1,222,325 - Pf6N2E2_1048 0.48 -0.0 1,222,325 - Pf6N2E2_1048 0.48 -0.5 1,222,325 - Pf6N2E2_1048 0.48 +0.5 1,222,325 - Pf6N2E2_1048 0.48 +0.6 1,222,325 - Pf6N2E2_1048 0.48 +0.3 1,222,325 - Pf6N2E2_1048 0.48 +0.3 1,222,325 - Pf6N2E2_1048 0.48 -0.3 1,222,581 + +0.4 1,222,581 + -0.9 1,222,581 + -0.5 1,222,581 + +0.1 1,222,581 + +0.6 1,222,581 + -0.6 1,222,581 + -0.0 1,222,582 - +1.0 1,222,582 - +0.5 1,222,594 + +0.4 1,222,594 + +0.1 1,222,594 + -0.5 1,222,595 - -0.1 1,222,597 - -0.2 1,222,698 + Pf6N2E2_1049 0.76 -0.6 1,222,699 - Pf6N2E2_1049 0.77 +0.5 1,222,699 - Pf6N2E2_1049 0.77 -0.5 1,222,706 + Pf6N2E2_1049 0.82 -0.6 1,222,706 + Pf6N2E2_1049 0.82 -1.5 1,222,706 + Pf6N2E2_1049 0.82 -0.4 1,222,707 - Pf6N2E2_1049 0.83 -0.6 1,222,708 + Pf6N2E2_1049 0.83 +0.1 1,222,709 - Pf6N2E2_1049 0.84 -0.2 1,222,859 + +0.5 1,222,860 - -0.1 1,222,860 - +1.0 1,222,860 - +0.0 1,222,889 + -0.3 1,222,889 + -0.1 1,222,889 + +0.6 1,222,890 - +1.0 1,222,890 - +1.7 1,222,890 - +0.7 1,222,890 - +0.2 1,222,890 - -0.7 1,222,915 + -0.9 1,222,915 + +0.5 1,222,915 + +0.4 1,222,915 + +0.0 1,222,916 - -0.1 1,222,916 - -0.2 1,222,916 - -0.3 1,222,922 + +0.0 1,222,979 + +0.7 1,223,102 + +0.4 1,223,156 + +0.3 1,223,156 + -0.6 1,223,156 + +0.6 1,223,292 + +0.6 1,223,292 + +0.1 1,223,292 + -1.7 1,223,292 + -0.7 1,223,342 + +0.4 1,223,350 + +0.6 1,223,350 + -1.4 1,223,351 - +0.8 1,223,360 + +0.0 1,223,360 + +0.4 1,223,388 + -0.0 1,223,388 + +0.6 1,223,388 + +0.8 1,223,388 + -0.3 1,223,388 + -0.5 1,223,388 + +0.2 1,223,388 + +0.4 1,223,388 + +0.2 1,223,392 + +0.3 1,223,412 + -0.1 1,223,424 + +1.4 1,223,529 + +0.2 1,223,529 + +0.6 1,223,529 + +0.1 1,223,539 + +0.2 1,223,571 + +0.3 1,223,589 + -0.2 1,223,589 + +0.6
Or see this region's nucleotide sequence