Experiment: formate N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03630 and MMJJ_RS03635 are separated by 135 nucleotides MMJJ_RS03635 and MMJJ_RS03640 are separated by 218 nucleotides
MMJJ_RS03630: MMJJ_RS03630 - AMP phosphorylase, at 675,584 to 677,101
_RS03630
MMJJ_RS03635: MMJJ_RS03635 - methionine--tRNA ligase, at 677,237 to 679,228
_RS03635
MMJJ_RS03640: MMJJ_RS03640 - type II glyceraldehyde-3-phosphate dehydrogenase, at 679,447 to 680,469
_RS03640
Position (kb) 677
678
679
680 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 676.255 kb on - strand, within MMJJ_RS03630 at 676.269 kb on - strand, within MMJJ_RS03630 at 676.269 kb on - strand, within MMJJ_RS03630 at 676.478 kb on - strand, within MMJJ_RS03630 at 676.479 kb on + strand, within MMJJ_RS03630 at 676.479 kb on + strand, within MMJJ_RS03630 at 676.554 kb on + strand, within MMJJ_RS03630 at 676.576 kb on + strand, within MMJJ_RS03630 at 676.576 kb on + strand, within MMJJ_RS03630 at 676.600 kb on - strand, within MMJJ_RS03630 at 676.680 kb on + strand, within MMJJ_RS03630 at 676.680 kb on + strand, within MMJJ_RS03630 at 676.703 kb on + strand, within MMJJ_RS03630 at 676.707 kb on - strand, within MMJJ_RS03630 at 676.707 kb on - strand, within MMJJ_RS03630 at 676.708 kb on + strand, within MMJJ_RS03630 at 676.708 kb on + strand, within MMJJ_RS03630 at 676.711 kb on - strand, within MMJJ_RS03630 at 676.711 kb on - strand, within MMJJ_RS03630 at 676.711 kb on - strand, within MMJJ_RS03630 at 676.712 kb on + strand, within MMJJ_RS03630 at 676.781 kb on - strand, within MMJJ_RS03630 at 676.781 kb on - strand, within MMJJ_RS03630 at 676.796 kb on - strand, within MMJJ_RS03630 at 676.825 kb on - strand, within MMJJ_RS03630 at 676.859 kb on - strand, within MMJJ_RS03630 at 676.860 kb on + strand, within MMJJ_RS03630 at 676.907 kb on - strand, within MMJJ_RS03630 at 676.907 kb on - strand, within MMJJ_RS03630 at 676.908 kb on + strand, within MMJJ_RS03630 at 676.949 kb on + strand, within MMJJ_RS03630 at 676.953 kb on - strand at 676.953 kb on - strand at 676.954 kb on + strand at 676.972 kb on - strand at 676.988 kb on - strand at 677.013 kb on - strand at 677.013 kb on - strand at 677.014 kb on + strand at 677.076 kb on + strand at 677.097 kb on + strand at 677.097 kb on + strand at 677.121 kb on + strand at 677.121 kb on + strand at 677.121 kb on + strand at 677.132 kb on - strand at 677.133 kb on + strand at 677.140 kb on - strand at 677.155 kb on - strand at 677.155 kb on - strand at 677.156 kb on + strand at 677.156 kb on + strand at 677.158 kb on + strand at 677.161 kb on - strand at 677.162 kb on + strand at 677.167 kb on + strand at 677.236 kb on + strand at 677.288 kb on - strand at 677.293 kb on - strand at 677.541 kb on - strand, within MMJJ_RS03635 at 677.554 kb on - strand, within MMJJ_RS03635 at 677.555 kb on + strand, within MMJJ_RS03635 at 677.623 kb on - strand, within MMJJ_RS03635 at 677.682 kb on + strand, within MMJJ_RS03635 at 677.720 kb on - strand, within MMJJ_RS03635 at 678.192 kb on + strand, within MMJJ_RS03635 at 678.492 kb on - strand, within MMJJ_RS03635 at 678.536 kb on - strand, within MMJJ_RS03635 at 678.819 kb on - strand at 679.137 kb on - strand at 679.155 kb on - strand at 679.268 kb on - strand at 679.297 kb on - strand at 679.297 kb on - strand at 679.298 kb on + strand at 679.298 kb on + strand at 679.302 kb on - strand at 679.302 kb on - strand at 679.302 kb on - strand at 679.302 kb on - strand at 679.305 kb on - strand at 679.306 kb on + strand at 679.306 kb on + strand at 679.308 kb on - strand at 679.308 kb on - strand at 679.308 kb on - strand at 679.308 kb on - strand at 679.309 kb on + strand at 679.312 kb on - strand at 679.350 kb on + strand at 679.351 kb on - strand at 679.351 kb on - strand at 679.351 kb on - strand at 679.352 kb on + strand at 679.356 kb on - strand at 679.356 kb on - strand at 679.356 kb on - strand at 679.357 kb on + strand at 679.360 kb on + strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.361 kb on - strand at 679.362 kb on + strand at 679.362 kb on + strand at 679.362 kb on + strand at 679.362 kb on + strand at 679.366 kb on + strand at 679.366 kb on + strand at 679.372 kb on - strand at 679.379 kb on + strand at 679.379 kb on + strand at 679.400 kb on + strand at 679.411 kb on + strand at 679.850 kb on + strand, within MMJJ_RS03640 at 679.862 kb on - strand, within MMJJ_RS03640 at 679.887 kb on - strand, within MMJJ_RS03640 at 679.897 kb on - strand, within MMJJ_RS03640
Per-strain Table
Position Strand Gene LocusTag Fraction formate N-free with 10mM Ammonium chloride remove 676,255 - MMJJ_RS03630 0.44 -1.9 676,269 - MMJJ_RS03630 0.45 -1.7 676,269 - MMJJ_RS03630 0.45 -1.0 676,478 - MMJJ_RS03630 0.59 -0.1 676,479 + MMJJ_RS03630 0.59 -2.7 676,479 + MMJJ_RS03630 0.59 -1.5 676,554 + MMJJ_RS03630 0.64 -2.2 676,576 + MMJJ_RS03630 0.65 -2.6 676,576 + MMJJ_RS03630 0.65 -2.0 676,600 - MMJJ_RS03630 0.67 -4.1 676,680 + MMJJ_RS03630 0.72 -1.4 676,680 + MMJJ_RS03630 0.72 -1.5 676,703 + MMJJ_RS03630 0.74 -1.9 676,707 - MMJJ_RS03630 0.74 -2.3 676,707 - MMJJ_RS03630 0.74 -1.8 676,708 + MMJJ_RS03630 0.74 -1.6 676,708 + MMJJ_RS03630 0.74 -1.8 676,711 - MMJJ_RS03630 0.74 -1.7 676,711 - MMJJ_RS03630 0.74 -1.0 676,711 - MMJJ_RS03630 0.74 -2.7 676,712 + MMJJ_RS03630 0.74 -2.1 676,781 - MMJJ_RS03630 0.79 -2.4 676,781 - MMJJ_RS03630 0.79 -0.1 676,796 - MMJJ_RS03630 0.80 -2.4 676,825 - MMJJ_RS03630 0.82 -1.4 676,859 - MMJJ_RS03630 0.84 -0.6 676,860 + MMJJ_RS03630 0.84 -1.3 676,907 - MMJJ_RS03630 0.87 -0.4 676,907 - MMJJ_RS03630 0.87 -1.6 676,908 + MMJJ_RS03630 0.87 -1.6 676,949 + MMJJ_RS03630 0.90 -1.9 676,953 - -2.2 676,953 - -2.0 676,954 + -2.2 676,972 - -3.4 676,988 - -0.4 677,013 - -2.0 677,013 - -2.2 677,014 + -0.9 677,076 + -1.1 677,097 + -2.7 677,097 + -2.3 677,121 + -1.9 677,121 + +0.5 677,121 + -3.0 677,132 - -1.8 677,133 + -1.8 677,140 - -1.3 677,155 - -2.5 677,155 - -3.6 677,156 + -0.2 677,156 + -1.4 677,158 + +0.1 677,161 - -2.2 677,162 + -1.4 677,167 + -0.8 677,236 + +0.3 677,288 - -0.0 677,293 - -0.7 677,541 - MMJJ_RS03635 0.15 -0.3 677,554 - MMJJ_RS03635 0.16 -2.8 677,555 + MMJJ_RS03635 0.16 -0.4 677,623 - MMJJ_RS03635 0.19 -0.6 677,682 + MMJJ_RS03635 0.22 -1.9 677,720 - MMJJ_RS03635 0.24 -1.9 678,192 + MMJJ_RS03635 0.48 -0.9 678,492 - MMJJ_RS03635 0.63 -2.6 678,536 - MMJJ_RS03635 0.65 -3.6 678,819 - +0.5 679,137 - -0.9 679,155 - -2.1 679,268 - -0.9 679,297 - -0.2 679,297 - -2.8 679,298 + +0.9 679,298 + -2.1 679,302 - -1.0 679,302 - +0.1 679,302 - -0.6 679,302 - -1.9 679,305 - -0.6 679,306 + -0.8 679,306 + +0.7 679,308 - -0.3 679,308 - +0.6 679,308 - -0.8 679,308 - +0.8 679,309 + -0.7 679,312 - +0.4 679,350 + +1.1 679,351 - +1.1 679,351 - +0.1 679,351 - +0.2 679,352 + +0.3 679,356 - -0.3 679,356 - -0.3 679,356 - -0.2 679,357 + +0.9 679,360 + -0.4 679,361 - -0.1 679,361 - -0.1 679,361 - +0.3 679,361 - -0.5 679,361 - -0.1 679,361 - -0.1 679,361 - +1.2 679,362 + +0.1 679,362 + -0.5 679,362 + +0.2 679,362 + +0.6 679,366 + -2.0 679,366 + -0.6 679,372 - -1.4 679,379 + -0.5 679,379 + +0.3 679,400 + +0.6 679,411 + -0.1 679,850 + MMJJ_RS03640 0.39 -2.8 679,862 - MMJJ_RS03640 0.41 -3.1 679,887 - MMJJ_RS03640 0.43 -0.7 679,897 - MMJJ_RS03640 0.44 +0.1
Or see this region's nucleotide sequence