Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05971 and H281DRAFT_05972 are separated by 136 nucleotides H281DRAFT_05972 and H281DRAFT_05973 are separated by 162 nucleotides H281DRAFT_05973 and H281DRAFT_05974 overlap by 4 nucleotides
H281DRAFT_05971: H281DRAFT_05971 - Uncharacterized membrane protein YoaK, UPF0700 family, at 113,669 to 114,481
_05971
H281DRAFT_05972: H281DRAFT_05972 - conserved hypothetical protein, at 114,618 to 114,932
_05972
H281DRAFT_05973: H281DRAFT_05973 - carbon-monoxide dehydrogenase small subunit, at 115,095 to 115,586
_05973
H281DRAFT_05974: H281DRAFT_05974 - carbon-monoxide dehydrogenase medium subunit, at 115,583 to 116,443
_05974
Position (kb) 114
115 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 113.887 kb on - strand, within H281DRAFT_05971 at 114.400 kb on - strand at 114.604 kb on + strand at 114.621 kb on + strand at 114.857 kb on - strand, within H281DRAFT_05972 at 114.857 kb on - strand, within H281DRAFT_05972 at 114.859 kb on + strand, within H281DRAFT_05972 at 114.896 kb on - strand, within H281DRAFT_05972 at 115.076 kb on - strand at 115.239 kb on - strand, within H281DRAFT_05973 at 115.614 kb on - strand at 115.888 kb on - strand, within H281DRAFT_05974
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 113,887 - H281DRAFT_05971 0.27 -3.8 114,400 - +1.4 114,604 + -0.5 114,621 + -2.5 114,857 - H281DRAFT_05972 0.76 -1.5 114,857 - H281DRAFT_05972 0.76 +0.8 114,859 + H281DRAFT_05972 0.77 -0.1 114,896 - H281DRAFT_05972 0.88 +1.2 115,076 - -0.3 115,239 - H281DRAFT_05973 0.29 -1.6 115,614 - +1.1 115,888 - H281DRAFT_05974 0.35 +1.7
Or see this region's nucleotide sequence