Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05510 and H281DRAFT_05511 are separated by 265 nucleotides H281DRAFT_05511 and H281DRAFT_05512 are separated by 275 nucleotides H281DRAFT_05512 and H281DRAFT_05513 overlap by 7 nucleotides
H281DRAFT_05510: H281DRAFT_05510 - Transposase, at 90,793 to 91,818
_05510
H281DRAFT_05511: H281DRAFT_05511 - acyl-CoA thioester hydrolase, at 92,084 to 92,536
_05511
H281DRAFT_05512: H281DRAFT_05512 - branched-chain amino acid transport system ATP-binding protein, at 92,812 to 93,516
_05512
H281DRAFT_05513: H281DRAFT_05513 - branched-chain amino acid transport system permease protein, at 93,510 to 95,303
_05513
Position (kb) 92
93 Strain fitness (log2 ratio) -2
-1
0
1
2 at 91.103 kb on + strand, within H281DRAFT_05510 at 91.248 kb on - strand, within H281DRAFT_05510 at 91.248 kb on - strand, within H281DRAFT_05510 at 91.350 kb on + strand, within H281DRAFT_05510 at 91.382 kb on + strand, within H281DRAFT_05510 at 91.382 kb on + strand, within H281DRAFT_05510 at 91.383 kb on - strand, within H281DRAFT_05510 at 91.411 kb on + strand, within H281DRAFT_05510 at 91.730 kb on + strand at 91.731 kb on - strand at 91.878 kb on - strand at 91.910 kb on + strand at 91.969 kb on + strand at 92.090 kb on + strand at 92.100 kb on - strand at 92.166 kb on + strand, within H281DRAFT_05511 at 92.182 kb on - strand, within H281DRAFT_05511 at 92.311 kb on - strand, within H281DRAFT_05511 at 92.416 kb on - strand, within H281DRAFT_05511 at 92.504 kb on + strand at 92.747 kb on + strand at 92.822 kb on + strand at 92.982 kb on + strand, within H281DRAFT_05512 at 93.019 kb on + strand, within H281DRAFT_05512 at 93.224 kb on + strand, within H281DRAFT_05512 at 93.224 kb on + strand, within H281DRAFT_05512 at 93.389 kb on - strand, within H281DRAFT_05512
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 91,103 + H281DRAFT_05510 0.30 -0.4 91,248 - H281DRAFT_05510 0.44 +0.8 91,248 - H281DRAFT_05510 0.44 +0.2 91,350 + H281DRAFT_05510 0.54 +0.5 91,382 + H281DRAFT_05510 0.57 -0.1 91,382 + H281DRAFT_05510 0.57 -0.3 91,383 - H281DRAFT_05510 0.58 -0.9 91,411 + H281DRAFT_05510 0.60 +0.2 91,730 + +1.4 91,731 - +0.2 91,878 - +1.8 91,910 + -0.2 91,969 + -1.4 92,090 + -0.9 92,100 - -0.4 92,166 + H281DRAFT_05511 0.18 -1.1 92,182 - H281DRAFT_05511 0.22 -1.3 92,311 - H281DRAFT_05511 0.50 +0.1 92,416 - H281DRAFT_05511 0.73 +0.8 92,504 + +0.9 92,747 + +0.9 92,822 + -1.9 92,982 + H281DRAFT_05512 0.24 +1.6 93,019 + H281DRAFT_05512 0.29 +0.3 93,224 + H281DRAFT_05512 0.58 -2.1 93,224 + H281DRAFT_05512 0.58 -0.1 93,389 - H281DRAFT_05512 0.82 -0.1
Or see this region's nucleotide sequence