Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05132 and H281DRAFT_05133 are separated by 116 nucleotides H281DRAFT_05133 and H281DRAFT_05134 are separated by 101 nucleotides
H281DRAFT_05132: H281DRAFT_05132 - 3-oxoacyl-[acyl-carrier protein] reductase, at 81,809 to 83,260
_05132
H281DRAFT_05133: H281DRAFT_05133 - acyl-CoA dehydrogenase, at 83,377 to 85,875
_05133
H281DRAFT_05134: H281DRAFT_05134 - transcriptional regulator, TetR family, at 85,977 to 86,810
_05134
Position (kb) 83
84
85
86 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 82.650 kb on + strand, within H281DRAFT_05132 at 82.650 kb on + strand, within H281DRAFT_05132 at 82.650 kb on + strand, within H281DRAFT_05132 at 82.651 kb on - strand, within H281DRAFT_05132 at 82.651 kb on - strand, within H281DRAFT_05132 at 82.720 kb on - strand, within H281DRAFT_05132 at 82.849 kb on + strand, within H281DRAFT_05132 at 83.248 kb on - strand at 83.261 kb on - strand at 83.261 kb on - strand at 83.711 kb on + strand, within H281DRAFT_05133 at 83.712 kb on - strand, within H281DRAFT_05133 at 84.125 kb on + strand, within H281DRAFT_05133 at 84.125 kb on + strand, within H281DRAFT_05133 at 84.311 kb on + strand, within H281DRAFT_05133 at 84.312 kb on - strand, within H281DRAFT_05133 at 84.312 kb on - strand, within H281DRAFT_05133 at 84.408 kb on - strand, within H281DRAFT_05133 at 84.509 kb on + strand, within H281DRAFT_05133 at 84.593 kb on + strand, within H281DRAFT_05133 at 84.593 kb on + strand, within H281DRAFT_05133 at 84.594 kb on - strand, within H281DRAFT_05133 at 84.936 kb on + strand, within H281DRAFT_05133 at 85.030 kb on - strand, within H281DRAFT_05133 at 85.031 kb on + strand, within H281DRAFT_05133 at 85.032 kb on - strand, within H281DRAFT_05133 at 85.194 kb on - strand, within H281DRAFT_05133 at 85.808 kb on - strand at 85.826 kb on + strand at 85.827 kb on - strand at 86.070 kb on - strand, within H281DRAFT_05134 at 86.392 kb on + strand, within H281DRAFT_05134 at 86.435 kb on + strand, within H281DRAFT_05134 at 86.660 kb on - strand, within H281DRAFT_05134 at 86.860 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 82,650 + H281DRAFT_05132 0.58 +0.6 82,650 + H281DRAFT_05132 0.58 -1.2 82,650 + H281DRAFT_05132 0.58 +1.9 82,651 - H281DRAFT_05132 0.58 -1.1 82,651 - H281DRAFT_05132 0.58 +0.3 82,720 - H281DRAFT_05132 0.63 +0.5 82,849 + H281DRAFT_05132 0.72 -0.2 83,248 - +0.2 83,261 - -1.9 83,261 - +0.9 83,711 + H281DRAFT_05133 0.13 +0.8 83,712 - H281DRAFT_05133 0.13 -0.8 84,125 + H281DRAFT_05133 0.30 +0.2 84,125 + H281DRAFT_05133 0.30 +0.6 84,311 + H281DRAFT_05133 0.37 -1.9 84,312 - H281DRAFT_05133 0.37 +0.3 84,312 - H281DRAFT_05133 0.37 +0.6 84,408 - H281DRAFT_05133 0.41 -3.2 84,509 + H281DRAFT_05133 0.45 +1.7 84,593 + H281DRAFT_05133 0.49 +0.3 84,593 + H281DRAFT_05133 0.49 -0.2 84,594 - H281DRAFT_05133 0.49 -0.2 84,936 + H281DRAFT_05133 0.62 +0.2 85,030 - H281DRAFT_05133 0.66 -0.1 85,031 + H281DRAFT_05133 0.66 +0.4 85,032 - H281DRAFT_05133 0.66 +1.2 85,194 - H281DRAFT_05133 0.73 -0.8 85,808 - -0.4 85,826 + -0.3 85,827 - +0.9 86,070 - H281DRAFT_05134 0.11 -1.8 86,392 + H281DRAFT_05134 0.50 +0.9 86,435 + H281DRAFT_05134 0.55 -0.2 86,660 - H281DRAFT_05134 0.82 +2.6 86,860 - +0.6
Or see this region's nucleotide sequence