Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05047 and H281DRAFT_05048 are separated by 68 nucleotides H281DRAFT_05048 and H281DRAFT_05049 overlap by 1 nucleotides H281DRAFT_05049 and H281DRAFT_05050 are separated by 361 nucleotides
H281DRAFT_05047: H281DRAFT_05047 - thioredoxin reductase (NADPH), at 235,921 to 237,612
_05047
H281DRAFT_05048: H281DRAFT_05048 - Tripartite tricarboxylate transporter TctA family protein, at 237,681 to 239,060
_05048
H281DRAFT_05049: H281DRAFT_05049 - hypothetical protein, at 239,060 to 239,560
_05049
H281DRAFT_05050: H281DRAFT_05050 - 3-hydroxy-3-methylglutaryl-coenzyme A reductase, at 239,922 to 241,208
_05050
Position (kb) 237
238
239
240 Strain fitness (log2 ratio) -2
-1
0
1
2 at 236.694 kb on - strand at 236.782 kb on + strand, within H281DRAFT_05047 at 236.783 kb on - strand, within H281DRAFT_05047 at 236.848 kb on - strand, within H281DRAFT_05047 at 237.032 kb on - strand, within H281DRAFT_05047 at 237.122 kb on + strand, within H281DRAFT_05047 at 237.153 kb on + strand, within H281DRAFT_05047 at 237.153 kb on + strand, within H281DRAFT_05047 at 237.153 kb on + strand, within H281DRAFT_05047 at 237.154 kb on - strand, within H281DRAFT_05047 at 237.154 kb on - strand, within H281DRAFT_05047 at 237.154 kb on - strand, within H281DRAFT_05047 at 237.154 kb on - strand, within H281DRAFT_05047 at 237.229 kb on - strand, within H281DRAFT_05047 at 237.229 kb on - strand, within H281DRAFT_05047 at 237.725 kb on - strand at 237.728 kb on - strand at 237.925 kb on + strand, within H281DRAFT_05048 at 237.926 kb on - strand, within H281DRAFT_05048 at 238.154 kb on - strand, within H281DRAFT_05048 at 238.154 kb on - strand, within H281DRAFT_05048 at 238.154 kb on - strand, within H281DRAFT_05048 at 238.207 kb on + strand, within H281DRAFT_05048 at 238.207 kb on + strand, within H281DRAFT_05048 at 238.208 kb on - strand, within H281DRAFT_05048 at 238.312 kb on + strand at 238.492 kb on + strand, within H281DRAFT_05048 at 238.569 kb on + strand, within H281DRAFT_05048 at 239.332 kb on - strand, within H281DRAFT_05049 at 239.417 kb on + strand, within H281DRAFT_05049 at 239.465 kb on + strand, within H281DRAFT_05049 at 239.466 kb on - strand, within H281DRAFT_05049 at 239.749 kb on + strand at 239.750 kb on - strand at 239.750 kb on - strand at 239.952 kb on + strand at 240.036 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 236,694 - +1.2 236,782 + H281DRAFT_05047 0.51 -0.4 236,783 - H281DRAFT_05047 0.51 -0.5 236,848 - H281DRAFT_05047 0.55 +1.1 237,032 - H281DRAFT_05047 0.66 +0.1 237,122 + H281DRAFT_05047 0.71 -1.5 237,153 + H281DRAFT_05047 0.73 -1.5 237,153 + H281DRAFT_05047 0.73 +1.1 237,153 + H281DRAFT_05047 0.73 -0.4 237,154 - H281DRAFT_05047 0.73 -2.0 237,154 - H281DRAFT_05047 0.73 -0.5 237,154 - H281DRAFT_05047 0.73 +0.6 237,154 - H281DRAFT_05047 0.73 -0.1 237,229 - H281DRAFT_05047 0.77 +0.0 237,229 - H281DRAFT_05047 0.77 -2.1 237,725 - -0.5 237,728 - -0.6 237,925 + H281DRAFT_05048 0.18 +0.1 237,926 - H281DRAFT_05048 0.18 +0.1 238,154 - H281DRAFT_05048 0.34 -0.0 238,154 - H281DRAFT_05048 0.34 +0.2 238,154 - H281DRAFT_05048 0.34 -0.6 238,207 + H281DRAFT_05048 0.38 -0.2 238,207 + H281DRAFT_05048 0.38 -0.7 238,208 - H281DRAFT_05048 0.38 +0.3 238,312 + +0.8 238,492 + H281DRAFT_05048 0.59 +2.4 238,569 + H281DRAFT_05048 0.64 +0.1 239,332 - H281DRAFT_05049 0.54 -0.4 239,417 + H281DRAFT_05049 0.71 +0.4 239,465 + H281DRAFT_05049 0.81 +2.4 239,466 - H281DRAFT_05049 0.81 +1.1 239,749 + -0.1 239,750 - -0.7 239,750 - +1.1 239,952 + -1.1 240,036 + -0.1
Or see this region's nucleotide sequence