Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04783 and H281DRAFT_04784 overlap by 4 nucleotides H281DRAFT_04784 and H281DRAFT_04785 are separated by 278 nucleotides
H281DRAFT_04783: H281DRAFT_04783 - Glycosyltransferase involved in cell wall bisynthesis, at 205,495 to 206,712
_04783
H281DRAFT_04784: H281DRAFT_04784 - O-antigen ligase like membrane protein, at 206,709 to 208,151
_04784
H281DRAFT_04785: H281DRAFT_04785 - Peptidoglycan/LPS O-acetylase OafA/YrhL, contains acyltransferase and SGNH-hydrolase domains, at 208,430 to 209,578
_04785
Position (kb) 206
207
208
209 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 206.009 kb on - strand, within H281DRAFT_04783 at 206.435 kb on + strand, within H281DRAFT_04783 at 206.504 kb on + strand, within H281DRAFT_04783 at 206.513 kb on + strand, within H281DRAFT_04783 at 206.513 kb on + strand, within H281DRAFT_04783 at 206.513 kb on + strand, within H281DRAFT_04783 at 206.513 kb on + strand, within H281DRAFT_04783 at 206.513 kb on + strand, within H281DRAFT_04783 at 206.513 kb on + strand, within H281DRAFT_04783 at 206.772 kb on - strand at 206.902 kb on + strand, within H281DRAFT_04784 at 206.978 kb on + strand, within H281DRAFT_04784 at 207.071 kb on - strand, within H281DRAFT_04784 at 207.238 kb on + strand, within H281DRAFT_04784 at 207.317 kb on - strand, within H281DRAFT_04784 at 207.887 kb on - strand, within H281DRAFT_04784 at 207.887 kb on - strand, within H281DRAFT_04784 at 207.930 kb on - strand, within H281DRAFT_04784 at 207.937 kb on - strand, within H281DRAFT_04784 at 208.090 kb on + strand at 208.107 kb on + strand at 208.111 kb on - strand at 208.296 kb on + strand at 208.365 kb on - strand at 208.555 kb on - strand, within H281DRAFT_04785 at 208.555 kb on - strand, within H281DRAFT_04785 at 208.661 kb on + strand, within H281DRAFT_04785 at 208.697 kb on - strand, within H281DRAFT_04785 at 208.734 kb on - strand, within H281DRAFT_04785 at 208.734 kb on - strand, within H281DRAFT_04785 at 208.780 kb on + strand, within H281DRAFT_04785 at 208.781 kb on - strand, within H281DRAFT_04785 at 208.942 kb on - strand, within H281DRAFT_04785 at 209.024 kb on - strand, within H281DRAFT_04785 at 209.024 kb on - strand, within H281DRAFT_04785 at 209.075 kb on + strand, within H281DRAFT_04785 at 209.076 kb on - strand, within H281DRAFT_04785
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 206,009 - H281DRAFT_04783 0.42 +0.5 206,435 + H281DRAFT_04783 0.77 +0.2 206,504 + H281DRAFT_04783 0.83 -0.5 206,513 + H281DRAFT_04783 0.84 -0.7 206,513 + H281DRAFT_04783 0.84 -1.1 206,513 + H281DRAFT_04783 0.84 -4.3 206,513 + H281DRAFT_04783 0.84 -0.9 206,513 + H281DRAFT_04783 0.84 +0.2 206,513 + H281DRAFT_04783 0.84 +0.6 206,772 - -1.5 206,902 + H281DRAFT_04784 0.13 +0.4 206,978 + H281DRAFT_04784 0.19 -0.2 207,071 - H281DRAFT_04784 0.25 +0.8 207,238 + H281DRAFT_04784 0.37 -0.6 207,317 - H281DRAFT_04784 0.42 +1.4 207,887 - H281DRAFT_04784 0.82 +0.2 207,887 - H281DRAFT_04784 0.82 -0.1 207,930 - H281DRAFT_04784 0.85 -0.5 207,937 - H281DRAFT_04784 0.85 +0.4 208,090 + +0.6 208,107 + +1.2 208,111 - +1.1 208,296 + -1.5 208,365 - +0.4 208,555 - H281DRAFT_04785 0.11 -0.3 208,555 - H281DRAFT_04785 0.11 -0.3 208,661 + H281DRAFT_04785 0.20 +1.0 208,697 - H281DRAFT_04785 0.23 +1.4 208,734 - H281DRAFT_04785 0.26 +1.1 208,734 - H281DRAFT_04785 0.26 +0.3 208,780 + H281DRAFT_04785 0.30 +0.9 208,781 - H281DRAFT_04785 0.31 -0.5 208,942 - H281DRAFT_04785 0.45 +0.4 209,024 - H281DRAFT_04785 0.52 -1.5 209,024 - H281DRAFT_04785 0.52 -0.7 209,075 + H281DRAFT_04785 0.56 -1.1 209,076 - H281DRAFT_04785 0.56 +1.0
Or see this region's nucleotide sequence