Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04779 and H281DRAFT_04780 are separated by 31 nucleotides H281DRAFT_04780 and H281DRAFT_04781 are separated by 183 nucleotides H281DRAFT_04781 and H281DRAFT_04782 are separated by 169 nucleotides
H281DRAFT_04779: H281DRAFT_04779 - colanic acid biosynthesis glycosyl transferase WcaI, at 200,838 to 202,169
_04779
H281DRAFT_04780: H281DRAFT_04780 - hypothetical protein, at 202,201 to 202,695
_04780
H281DRAFT_04781: H281DRAFT_04781 - putative colanic acid biosynthesis acetyltransferase WcaF, at 202,879 to 203,493
_04781
H281DRAFT_04782: H281DRAFT_04782 - Choline dehydrogenase, at 203,663 to 205,396
_04782
Position (kb) 202
203 Strain fitness (log2 ratio) -2
-1
0
1 at 201.258 kb on + strand, within H281DRAFT_04779 at 201.259 kb on - strand, within H281DRAFT_04779 at 202.022 kb on + strand, within H281DRAFT_04779 at 202.023 kb on - strand, within H281DRAFT_04779 at 202.142 kb on + strand at 202.253 kb on + strand, within H281DRAFT_04780 at 202.254 kb on - strand, within H281DRAFT_04780 at 202.675 kb on - strand at 202.710 kb on + strand at 202.711 kb on - strand at 202.845 kb on - strand at 203.010 kb on - strand, within H281DRAFT_04781
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 201,258 + H281DRAFT_04779 0.32 +1.2 201,259 - H281DRAFT_04779 0.32 +1.1 202,022 + H281DRAFT_04779 0.89 -0.3 202,023 - H281DRAFT_04779 0.89 +1.5 202,142 + +1.5 202,253 + H281DRAFT_04780 0.11 +1.5 202,254 - H281DRAFT_04780 0.11 +1.4 202,675 - +0.4 202,710 + +1.2 202,711 - -0.2 202,845 - -2.0 203,010 - H281DRAFT_04781 0.21 +0.8
Or see this region's nucleotide sequence