Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04737 and H281DRAFT_04738 are separated by 94 nucleotides H281DRAFT_04738 and H281DRAFT_04739 are separated by 102 nucleotides
H281DRAFT_04737: H281DRAFT_04737 - glutaryl-CoA dehydrogenase, at 144,707 to 145,897
_04737
H281DRAFT_04738: H281DRAFT_04738 - transcriptional regulator, IclR family, at 145,992 to 146,777
_04738
H281DRAFT_04739: H281DRAFT_04739 - EAL domain, c-di-GMP-specific phosphodiesterase class I (or its enzymatically inactive variant), at 146,880 to 147,713
_04739
Position (kb) 145
146
147 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 145.057 kb on + strand, within H281DRAFT_04737 at 145.072 kb on - strand, within H281DRAFT_04737 at 145.132 kb on - strand, within H281DRAFT_04737 at 145.261 kb on + strand, within H281DRAFT_04737 at 145.261 kb on + strand, within H281DRAFT_04737 at 145.471 kb on + strand, within H281DRAFT_04737 at 145.472 kb on - strand, within H281DRAFT_04737 at 145.527 kb on + strand at 145.527 kb on + strand, within H281DRAFT_04737 at 145.650 kb on + strand, within H281DRAFT_04737 at 145.651 kb on - strand, within H281DRAFT_04737 at 145.651 kb on - strand, within H281DRAFT_04737 at 145.659 kb on + strand, within H281DRAFT_04737 at 145.659 kb on + strand, within H281DRAFT_04737 at 145.680 kb on + strand, within H281DRAFT_04737 at 145.680 kb on + strand, within H281DRAFT_04737 at 145.681 kb on - strand, within H281DRAFT_04737 at 145.769 kb on + strand, within H281DRAFT_04737 at 145.769 kb on + strand, within H281DRAFT_04737 at 145.770 kb on - strand, within H281DRAFT_04737 at 145.800 kb on + strand at 145.902 kb on + strand at 145.902 kb on + strand at 145.902 kb on + strand at 145.903 kb on - strand at 145.984 kb on - strand at 146.164 kb on - strand, within H281DRAFT_04738 at 146.165 kb on + strand, within H281DRAFT_04738 at 146.184 kb on + strand, within H281DRAFT_04738 at 146.317 kb on + strand, within H281DRAFT_04738 at 146.318 kb on - strand, within H281DRAFT_04738 at 146.364 kb on + strand, within H281DRAFT_04738 at 146.365 kb on - strand, within H281DRAFT_04738 at 146.457 kb on + strand, within H281DRAFT_04738 at 146.457 kb on + strand, within H281DRAFT_04738 at 146.458 kb on - strand, within H281DRAFT_04738 at 146.593 kb on - strand, within H281DRAFT_04738 at 146.876 kb on + strand at 146.876 kb on + strand at 146.876 kb on + strand at 146.877 kb on - strand at 146.900 kb on - strand at 146.942 kb on - strand at 146.942 kb on - strand at 147.152 kb on - strand, within H281DRAFT_04739 at 147.161 kb on - strand, within H281DRAFT_04739 at 147.191 kb on + strand, within H281DRAFT_04739 at 147.226 kb on - strand, within H281DRAFT_04739 at 147.287 kb on - strand, within H281DRAFT_04739 at 147.315 kb on + strand, within H281DRAFT_04739 at 147.322 kb on - strand, within H281DRAFT_04739 at 147.327 kb on + strand, within H281DRAFT_04739 at 147.441 kb on - strand, within H281DRAFT_04739 at 147.715 kb on + strand at 147.728 kb on - strand at 147.733 kb on - strand at 147.733 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 145,057 + H281DRAFT_04737 0.29 -1.2 145,072 - H281DRAFT_04737 0.31 -3.1 145,132 - H281DRAFT_04737 0.36 -0.0 145,261 + H281DRAFT_04737 0.47 +0.1 145,261 + H281DRAFT_04737 0.47 +0.5 145,471 + H281DRAFT_04737 0.64 -0.2 145,472 - H281DRAFT_04737 0.64 -0.8 145,527 + -0.6 145,527 + H281DRAFT_04737 0.69 +0.1 145,650 + H281DRAFT_04737 0.79 -2.8 145,651 - H281DRAFT_04737 0.79 +0.4 145,651 - H281DRAFT_04737 0.79 -1.4 145,659 + H281DRAFT_04737 0.80 +0.2 145,659 + H281DRAFT_04737 0.80 +0.2 145,680 + H281DRAFT_04737 0.82 +0.3 145,680 + H281DRAFT_04737 0.82 +0.5 145,681 - H281DRAFT_04737 0.82 +1.0 145,769 + H281DRAFT_04737 0.89 -0.3 145,769 + H281DRAFT_04737 0.89 +0.4 145,770 - H281DRAFT_04737 0.89 -0.3 145,800 + +0.5 145,902 + -0.1 145,902 + -2.8 145,902 + +0.4 145,903 - -0.2 145,984 - +0.4 146,164 - H281DRAFT_04738 0.22 +0.1 146,165 + H281DRAFT_04738 0.22 -0.1 146,184 + H281DRAFT_04738 0.24 +1.0 146,317 + H281DRAFT_04738 0.41 +0.7 146,318 - H281DRAFT_04738 0.41 +0.7 146,364 + H281DRAFT_04738 0.47 -0.5 146,365 - H281DRAFT_04738 0.47 +0.0 146,457 + H281DRAFT_04738 0.59 -0.5 146,457 + H281DRAFT_04738 0.59 -0.1 146,458 - H281DRAFT_04738 0.59 -0.4 146,593 - H281DRAFT_04738 0.76 -0.5 146,876 + -0.5 146,876 + +0.1 146,876 + +0.3 146,877 - +1.5 146,900 - -0.4 146,942 - +1.0 146,942 - +0.2 147,152 - H281DRAFT_04739 0.33 +1.0 147,161 - H281DRAFT_04739 0.34 -0.4 147,191 + H281DRAFT_04739 0.37 +0.0 147,226 - H281DRAFT_04739 0.41 -0.2 147,287 - H281DRAFT_04739 0.49 -0.4 147,315 + H281DRAFT_04739 0.52 +0.1 147,322 - H281DRAFT_04739 0.53 -0.9 147,327 + H281DRAFT_04739 0.54 -0.0 147,441 - H281DRAFT_04739 0.67 -1.1 147,715 + -0.8 147,728 - -0.2 147,733 - +0.8 147,733 - -0.6
Or see this region's nucleotide sequence