Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04714 and H281DRAFT_04715 are separated by 176 nucleotides H281DRAFT_04715 and H281DRAFT_04716 are separated by 272 nucleotides H281DRAFT_04716 and H281DRAFT_04717 are separated by 201 nucleotides H281DRAFT_04717 and H281DRAFT_04718 are separated by 19 nucleotides
H281DRAFT_04714: H281DRAFT_04714 - small conductance mechanosensitive channel, at 128,277 to 129,089
_04714
H281DRAFT_04715: H281DRAFT_04715 - hypothetical protein, at 129,266 to 129,730
_04715
H281DRAFT_04716: H281DRAFT_04716 - tRNA_Lys_TTT, at 130,003 to 130,078
_04716
H281DRAFT_04717: H281DRAFT_04717 - tol-pal system protein YbgF, at 130,280 to 131,029
_04717
H281DRAFT_04718: H281DRAFT_04718 - peptidoglycan-associated lipoprotein, at 131,049 to 131,558
_04718
Position (kb)
130
131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 129.126 kb on - strand at 129.200 kb on + strand at 129.201 kb on - strand at 129.202 kb on + strand at 129.202 kb on + strand at 129.202 kb on + strand at 129.203 kb on - strand at 129.268 kb on - strand at 129.396 kb on + strand, within H281DRAFT_04715 at 129.397 kb on - strand, within H281DRAFT_04715 at 129.565 kb on + strand, within H281DRAFT_04715 at 129.640 kb on + strand, within H281DRAFT_04715 at 129.741 kb on + strand at 129.741 kb on + strand at 129.742 kb on - strand at 129.742 kb on - strand at 129.742 kb on - strand at 129.742 kb on - strand at 129.742 kb on - strand at 129.742 kb on - strand at 129.742 kb on - strand at 129.912 kb on + strand at 129.913 kb on - strand at 129.913 kb on - strand at 129.913 kb on - strand at 129.921 kb on - strand at 129.970 kb on - strand at 130.144 kb on + strand at 130.144 kb on + strand at 130.144 kb on + strand at 130.145 kb on - strand at 130.145 kb on - strand at 130.145 kb on - strand at 130.281 kb on + strand at 130.282 kb on - strand at 130.282 kb on - strand at 130.489 kb on - strand, within H281DRAFT_04717 at 130.503 kb on + strand, within H281DRAFT_04717 at 130.524 kb on + strand, within H281DRAFT_04717 at 130.558 kb on - strand, within H281DRAFT_04717 at 130.731 kb on + strand, within H281DRAFT_04717 at 130.735 kb on - strand, within H281DRAFT_04717 at 131.050 kb on + strand at 131.051 kb on - strand at 131.051 kb on - strand at 131.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 129,126 - -0.9 129,200 + +0.5 129,201 - -0.1 129,202 + +3.3 129,202 + +0.4 129,202 + -0.4 129,203 - -1.3 129,268 - -0.5 129,396 + H281DRAFT_04715 0.28 +0.2 129,397 - H281DRAFT_04715 0.28 +0.5 129,565 + H281DRAFT_04715 0.64 -0.9 129,640 + H281DRAFT_04715 0.80 +0.3 129,741 + +0.2 129,741 + +0.6 129,742 - -0.5 129,742 - -0.6 129,742 - -0.3 129,742 - +0.5 129,742 - -0.2 129,742 - +0.0 129,742 - +1.6 129,912 + -0.5 129,913 - -1.6 129,913 - -0.8 129,913 - +1.4 129,921 - -0.8 129,970 - +1.0 130,144 + +0.3 130,144 + +0.0 130,144 + -0.2 130,145 - +0.4 130,145 - +0.4 130,145 - +0.1 130,281 + +1.0 130,282 - +0.9 130,282 - +1.7 130,489 - H281DRAFT_04717 0.28 -0.8 130,503 + H281DRAFT_04717 0.30 -2.6 130,524 + H281DRAFT_04717 0.33 -2.2 130,558 - H281DRAFT_04717 0.37 -0.3 130,731 + H281DRAFT_04717 0.60 +0.3 130,735 - H281DRAFT_04717 0.61 -3.1 131,050 + -0.6 131,051 - +0.1 131,051 - -0.2 131,051 - -1.7
Or see this region's nucleotide sequence