Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04497 and H281DRAFT_04498 are separated by 80 nucleotides H281DRAFT_04498 and H281DRAFT_04499 are separated by 69 nucleotides
H281DRAFT_04497: H281DRAFT_04497 - Pimeloyl-ACP methyl ester carboxylesterase, at 229,545 to 230,456
_04497
H281DRAFT_04498: H281DRAFT_04498 - Enamine deaminase RidA, house cleaning of reactive enamine intermediates, YjgF/YER057c/UK114 family, at 230,537 to 230,890
_04498
H281DRAFT_04499: H281DRAFT_04499 - GTP pyrophosphokinase, at 230,960 to 233,203
_04499
Position (kb) 230
231 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 229.596 kb on + strand at 229.596 kb on + strand at 229.596 kb on + strand at 229.597 kb on - strand at 229.597 kb on - strand at 229.638 kb on + strand, within H281DRAFT_04497 at 229.638 kb on + strand, within H281DRAFT_04497 at 229.746 kb on + strand at 229.746 kb on + strand, within H281DRAFT_04497 at 229.746 kb on + strand, within H281DRAFT_04497 at 229.747 kb on - strand at 229.810 kb on + strand, within H281DRAFT_04497 at 229.810 kb on + strand, within H281DRAFT_04497 at 229.810 kb on + strand, within H281DRAFT_04497 at 229.810 kb on + strand, within H281DRAFT_04497 at 229.810 kb on + strand, within H281DRAFT_04497 at 229.810 kb on + strand, within H281DRAFT_04497 at 229.811 kb on - strand, within H281DRAFT_04497 at 229.811 kb on - strand, within H281DRAFT_04497 at 229.811 kb on - strand, within H281DRAFT_04497 at 229.827 kb on + strand, within H281DRAFT_04497 at 229.828 kb on - strand, within H281DRAFT_04497 at 229.828 kb on - strand, within H281DRAFT_04497 at 229.828 kb on - strand, within H281DRAFT_04497 at 229.961 kb on - strand, within H281DRAFT_04497 at 229.961 kb on - strand, within H281DRAFT_04497 at 230.124 kb on - strand, within H281DRAFT_04497 at 230.201 kb on + strand, within H281DRAFT_04497 at 230.236 kb on + strand, within H281DRAFT_04497 at 230.454 kb on + strand at 230.455 kb on - strand at 230.455 kb on - strand at 230.457 kb on + strand at 230.457 kb on + strand at 230.458 kb on - strand at 230.458 kb on - strand at 230.458 kb on - strand at 230.517 kb on + strand at 230.517 kb on + strand at 230.517 kb on + strand at 230.518 kb on - strand at 230.518 kb on - strand at 230.522 kb on - strand at 230.604 kb on - strand, within H281DRAFT_04498 at 230.707 kb on + strand, within H281DRAFT_04498 at 230.735 kb on + strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 231.384 kb on + strand, within H281DRAFT_04499 at 231.394 kb on - strand, within H281DRAFT_04499 at 231.429 kb on - strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.485 kb on + strand, within H281DRAFT_04499 at 231.486 kb on - strand, within H281DRAFT_04499 at 231.486 kb on - strand, within H281DRAFT_04499 at 231.486 kb on - strand, within H281DRAFT_04499 at 231.572 kb on + strand, within H281DRAFT_04499 at 231.572 kb on + strand, within H281DRAFT_04499 at 231.572 kb on + strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.573 kb on - strand, within H281DRAFT_04499 at 231.574 kb on + strand, within H281DRAFT_04499 at 231.575 kb on - strand, within H281DRAFT_04499 at 231.575 kb on - strand, within H281DRAFT_04499 at 231.623 kb on + strand, within H281DRAFT_04499 at 231.623 kb on + strand, within H281DRAFT_04499 at 231.624 kb on - strand, within H281DRAFT_04499 at 231.624 kb on - strand, within H281DRAFT_04499 at 231.624 kb on - strand, within H281DRAFT_04499 at 231.624 kb on - strand, within H281DRAFT_04499 at 231.687 kb on + strand, within H281DRAFT_04499 at 231.706 kb on + strand, within H281DRAFT_04499 at 231.707 kb on - strand, within H281DRAFT_04499 at 231.707 kb on - strand, within H281DRAFT_04499 at 231.707 kb on - strand, within H281DRAFT_04499 at 231.707 kb on - strand, within H281DRAFT_04499 at 231.707 kb on - strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.710 kb on + strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.711 kb on - strand, within H281DRAFT_04499 at 231.712 kb on + strand, within H281DRAFT_04499 at 231.712 kb on + strand, within H281DRAFT_04499 at 231.712 kb on + strand, within H281DRAFT_04499 at 231.712 kb on + strand, within H281DRAFT_04499 at 231.869 kb on + strand, within H281DRAFT_04499 at 231.870 kb on - strand, within H281DRAFT_04499 at 231.870 kb on - strand, within H281DRAFT_04499
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 229,596 + +0.7 229,596 + +0.7 229,596 + -0.7 229,597 - +0.5 229,597 - -0.7 229,638 + H281DRAFT_04497 0.10 -0.0 229,638 + H281DRAFT_04497 0.10 +0.0 229,746 + +0.9 229,746 + H281DRAFT_04497 0.22 -0.1 229,746 + H281DRAFT_04497 0.22 +2.1 229,747 - -0.7 229,810 + H281DRAFT_04497 0.29 -0.2 229,810 + H281DRAFT_04497 0.29 +0.5 229,810 + H281DRAFT_04497 0.29 -0.0 229,810 + H281DRAFT_04497 0.29 +0.1 229,810 + H281DRAFT_04497 0.29 -0.3 229,810 + H281DRAFT_04497 0.29 +0.1 229,811 - H281DRAFT_04497 0.29 +0.6 229,811 - H281DRAFT_04497 0.29 -0.2 229,811 - H281DRAFT_04497 0.29 +0.7 229,827 + H281DRAFT_04497 0.31 +0.3 229,828 - H281DRAFT_04497 0.31 +0.6 229,828 - H281DRAFT_04497 0.31 -0.3 229,828 - H281DRAFT_04497 0.31 +0.2 229,961 - H281DRAFT_04497 0.46 +0.5 229,961 - H281DRAFT_04497 0.46 -0.3 230,124 - H281DRAFT_04497 0.63 -0.0 230,201 + H281DRAFT_04497 0.72 +0.2 230,236 + H281DRAFT_04497 0.76 -0.0 230,454 + -0.3 230,455 - -0.0 230,455 - -0.4 230,457 + -0.9 230,457 + -0.6 230,458 - -0.6 230,458 - -1.1 230,458 - -2.9 230,517 + -0.4 230,517 + +0.9 230,517 + +0.6 230,518 - -1.6 230,518 - -1.7 230,522 - +0.0 230,604 - H281DRAFT_04498 0.19 -2.5 230,707 + H281DRAFT_04498 0.48 -0.6 230,735 + H281DRAFT_04498 0.56 -0.5 230,736 - H281DRAFT_04498 0.56 -1.4 230,736 - H281DRAFT_04498 0.56 -1.2 230,736 - H281DRAFT_04498 0.56 -0.9 230,736 - H281DRAFT_04498 0.56 -0.7 230,738 - H281DRAFT_04498 0.57 -0.5 230,738 - H281DRAFT_04498 0.57 -1.2 230,738 - H281DRAFT_04498 0.57 -0.3 230,738 - H281DRAFT_04498 0.57 -4.1 230,738 - H281DRAFT_04498 0.57 -2.8 231,384 + H281DRAFT_04499 0.19 +0.1 231,394 - H281DRAFT_04499 0.19 -4.2 231,429 - H281DRAFT_04499 0.21 -0.9 231,485 + H281DRAFT_04499 0.23 -1.0 231,485 + H281DRAFT_04499 0.23 -2.5 231,485 + H281DRAFT_04499 0.23 -2.4 231,485 + H281DRAFT_04499 0.23 -1.1 231,485 + H281DRAFT_04499 0.23 -1.4 231,485 + H281DRAFT_04499 0.23 -0.9 231,485 + H281DRAFT_04499 0.23 -2.9 231,485 + H281DRAFT_04499 0.23 -2.6 231,485 + H281DRAFT_04499 0.23 -1.4 231,486 - H281DRAFT_04499 0.23 +0.2 231,486 - H281DRAFT_04499 0.23 +0.0 231,486 - H281DRAFT_04499 0.23 -1.6 231,572 + H281DRAFT_04499 0.27 -1.7 231,572 + H281DRAFT_04499 0.27 -1.1 231,572 + H281DRAFT_04499 0.27 -0.2 231,573 - H281DRAFT_04499 0.27 -2.0 231,573 - H281DRAFT_04499 0.27 -2.0 231,573 - H281DRAFT_04499 0.27 -2.8 231,573 - H281DRAFT_04499 0.27 -3.3 231,573 - H281DRAFT_04499 0.27 -1.8 231,573 - H281DRAFT_04499 0.27 -5.2 231,573 - H281DRAFT_04499 0.27 -1.3 231,574 + H281DRAFT_04499 0.27 -3.2 231,575 - H281DRAFT_04499 0.27 -2.0 231,575 - H281DRAFT_04499 0.27 -0.6 231,623 + H281DRAFT_04499 0.30 -1.4 231,623 + H281DRAFT_04499 0.30 -0.9 231,624 - H281DRAFT_04499 0.30 -1.5 231,624 - H281DRAFT_04499 0.30 -1.6 231,624 - H281DRAFT_04499 0.30 -3.3 231,624 - H281DRAFT_04499 0.30 -0.9 231,687 + H281DRAFT_04499 0.32 -1.2 231,706 + H281DRAFT_04499 0.33 -3.9 231,707 - H281DRAFT_04499 0.33 -1.0 231,707 - H281DRAFT_04499 0.33 -0.6 231,707 - H281DRAFT_04499 0.33 -1.9 231,707 - H281DRAFT_04499 0.33 -0.7 231,707 - H281DRAFT_04499 0.33 -2.4 231,710 + H281DRAFT_04499 0.33 -1.8 231,710 + H281DRAFT_04499 0.33 -0.5 231,710 + H281DRAFT_04499 0.33 -1.9 231,710 + H281DRAFT_04499 0.33 -0.1 231,710 + H281DRAFT_04499 0.33 -1.3 231,710 + H281DRAFT_04499 0.33 -0.3 231,710 + H281DRAFT_04499 0.33 -2.4 231,710 + H281DRAFT_04499 0.33 +0.1 231,711 - H281DRAFT_04499 0.33 -0.8 231,711 - H281DRAFT_04499 0.33 -2.8 231,711 - H281DRAFT_04499 0.33 -1.0 231,711 - H281DRAFT_04499 0.33 -1.9 231,711 - H281DRAFT_04499 0.33 -0.0 231,711 - H281DRAFT_04499 0.33 -5.1 231,712 + H281DRAFT_04499 0.34 -0.7 231,712 + H281DRAFT_04499 0.34 -0.8 231,712 + H281DRAFT_04499 0.34 -1.9 231,712 + H281DRAFT_04499 0.34 -2.8 231,869 + H281DRAFT_04499 0.41 -1.9 231,870 - H281DRAFT_04499 0.41 -0.6 231,870 - H281DRAFT_04499 0.41 -1.2
Or see this region's nucleotide sequence