Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04339 and H281DRAFT_04340 are separated by 733 nucleotides H281DRAFT_04340 and H281DRAFT_04341 overlap by 8 nucleotides H281DRAFT_04341 and H281DRAFT_04342 overlap by 4 nucleotides
H281DRAFT_04339: H281DRAFT_04339 - RNAse E (EC 3.1.26.12), at 55,501 to 58,824
_04339
H281DRAFT_04340: H281DRAFT_04340 - 23S rRNA pseudouridine955/2504/2580 synthase, at 59,558 to 60,595
_04340
H281DRAFT_04341: H281DRAFT_04341 - phosphoglycolate phosphatase, at 60,588 to 61,247
_04341
H281DRAFT_04342: H281DRAFT_04342 - Ferredoxin subunit of nitrite reductase or a ring-hydroxylating dioxygenase, at 61,244 to 61,660
_04342
Position (kb) 59
60
61 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 58.914 kb on - strand at 59.466 kb on - strand at 59.479 kb on - strand at 59.479 kb on - strand at 59.479 kb on - strand at 59.549 kb on - strand at 59.550 kb on + strand at 59.550 kb on + strand at 59.550 kb on + strand at 59.577 kb on + strand at 59.686 kb on + strand, within H281DRAFT_04340 at 59.687 kb on - strand, within H281DRAFT_04340 at 59.711 kb on + strand, within H281DRAFT_04340 at 59.711 kb on + strand, within H281DRAFT_04340 at 59.711 kb on + strand, within H281DRAFT_04340 at 59.712 kb on - strand, within H281DRAFT_04340 at 60.039 kb on + strand, within H281DRAFT_04340 at 60.039 kb on + strand, within H281DRAFT_04340 at 60.039 kb on + strand, within H281DRAFT_04340 at 60.039 kb on + strand, within H281DRAFT_04340 at 60.039 kb on + strand, within H281DRAFT_04340 at 60.039 kb on + strand, within H281DRAFT_04340 at 60.040 kb on - strand, within H281DRAFT_04340 at 60.040 kb on - strand, within H281DRAFT_04340 at 60.040 kb on - strand, within H281DRAFT_04340 at 60.107 kb on - strand, within H281DRAFT_04340 at 60.107 kb on - strand, within H281DRAFT_04340 at 60.113 kb on + strand, within H281DRAFT_04340 at 60.114 kb on - strand, within H281DRAFT_04340 at 60.114 kb on - strand, within H281DRAFT_04340 at 60.116 kb on + strand, within H281DRAFT_04340 at 60.116 kb on + strand, within H281DRAFT_04340 at 60.116 kb on + strand, within H281DRAFT_04340 at 60.116 kb on + strand, within H281DRAFT_04340 at 60.116 kb on + strand, within H281DRAFT_04340 at 60.116 kb on + strand, within H281DRAFT_04340 at 60.117 kb on - strand, within H281DRAFT_04340 at 60.117 kb on - strand, within H281DRAFT_04340 at 60.188 kb on + strand, within H281DRAFT_04340 at 60.188 kb on + strand, within H281DRAFT_04340 at 60.281 kb on + strand, within H281DRAFT_04340 at 60.555 kb on + strand at 60.593 kb on + strand at 60.593 kb on + strand at 60.726 kb on + strand, within H281DRAFT_04341 at 60.726 kb on + strand, within H281DRAFT_04341 at 60.726 kb on + strand, within H281DRAFT_04341 at 60.726 kb on + strand, within H281DRAFT_04341 at 60.726 kb on + strand, within H281DRAFT_04341 at 60.726 kb on + strand, within H281DRAFT_04341 at 60.726 kb on + strand, within H281DRAFT_04341 at 60.726 kb on + strand, within H281DRAFT_04341 at 60.727 kb on - strand, within H281DRAFT_04341 at 60.727 kb on - strand, within H281DRAFT_04341 at 60.825 kb on + strand, within H281DRAFT_04341 at 60.825 kb on + strand, within H281DRAFT_04341 at 60.826 kb on - strand, within H281DRAFT_04341 at 60.901 kb on - strand, within H281DRAFT_04341 at 61.149 kb on + strand, within H281DRAFT_04341 at 61.150 kb on - strand, within H281DRAFT_04341 at 61.150 kb on - strand, within H281DRAFT_04341 at 61.194 kb on + strand at 61.194 kb on + strand at 61.194 kb on + strand at 61.194 kb on + strand at 61.194 kb on + strand at 61.195 kb on - strand at 61.195 kb on - strand at 61.195 kb on - strand at 61.279 kb on + strand at 61.280 kb on - strand at 61.413 kb on - strand, within H281DRAFT_04342 at 61.413 kb on - strand at 61.486 kb on - strand, within H281DRAFT_04342 at 61.488 kb on - strand, within H281DRAFT_04342 at 61.585 kb on - strand, within H281DRAFT_04342
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 58,914 - +0.7 59,466 - -0.1 59,479 - -1.8 59,479 - -0.9 59,479 - -2.2 59,549 - -2.4 59,550 + +1.7 59,550 + +2.6 59,550 + +0.8 59,577 + +0.8 59,686 + H281DRAFT_04340 0.12 -0.3 59,687 - H281DRAFT_04340 0.12 -0.6 59,711 + H281DRAFT_04340 0.15 +0.8 59,711 + H281DRAFT_04340 0.15 -0.4 59,711 + H281DRAFT_04340 0.15 -1.4 59,712 - H281DRAFT_04340 0.15 -1.2 60,039 + H281DRAFT_04340 0.46 +1.3 60,039 + H281DRAFT_04340 0.46 -0.3 60,039 + H281DRAFT_04340 0.46 -1.1 60,039 + H281DRAFT_04340 0.46 -0.1 60,039 + H281DRAFT_04340 0.46 +0.4 60,039 + H281DRAFT_04340 0.46 -1.4 60,040 - H281DRAFT_04340 0.46 -2.4 60,040 - H281DRAFT_04340 0.46 -1.4 60,040 - H281DRAFT_04340 0.46 -2.1 60,107 - H281DRAFT_04340 0.53 +1.2 60,107 - H281DRAFT_04340 0.53 +0.5 60,113 + H281DRAFT_04340 0.53 -2.1 60,114 - H281DRAFT_04340 0.54 +0.5 60,114 - H281DRAFT_04340 0.54 +1.0 60,116 + H281DRAFT_04340 0.54 -0.4 60,116 + H281DRAFT_04340 0.54 +0.1 60,116 + H281DRAFT_04340 0.54 -0.9 60,116 + H281DRAFT_04340 0.54 -0.6 60,116 + H281DRAFT_04340 0.54 -0.6 60,116 + H281DRAFT_04340 0.54 -0.6 60,117 - H281DRAFT_04340 0.54 -1.1 60,117 - H281DRAFT_04340 0.54 -0.2 60,188 + H281DRAFT_04340 0.61 -1.4 60,188 + H281DRAFT_04340 0.61 -0.9 60,281 + H281DRAFT_04340 0.70 +1.3 60,555 + -0.1 60,593 + +0.4 60,593 + -0.2 60,726 + H281DRAFT_04341 0.21 +0.6 60,726 + H281DRAFT_04341 0.21 -0.7 60,726 + H281DRAFT_04341 0.21 -2.3 60,726 + H281DRAFT_04341 0.21 +0.9 60,726 + H281DRAFT_04341 0.21 +1.3 60,726 + H281DRAFT_04341 0.21 -1.5 60,726 + H281DRAFT_04341 0.21 -2.1 60,726 + H281DRAFT_04341 0.21 -0.2 60,727 - H281DRAFT_04341 0.21 -1.1 60,727 - H281DRAFT_04341 0.21 +0.5 60,825 + H281DRAFT_04341 0.36 +0.6 60,825 + H281DRAFT_04341 0.36 -0.4 60,826 - H281DRAFT_04341 0.36 -3.1 60,901 - H281DRAFT_04341 0.47 +0.1 61,149 + H281DRAFT_04341 0.85 -0.8 61,150 - H281DRAFT_04341 0.85 -0.7 61,150 - H281DRAFT_04341 0.85 -1.0 61,194 + +1.6 61,194 + -1.6 61,194 + -0.6 61,194 + -0.3 61,194 + -0.1 61,195 - -0.2 61,195 - +0.2 61,195 - -0.7 61,279 + +0.0 61,280 - +0.5 61,413 - H281DRAFT_04342 0.41 +0.2 61,413 - +0.9 61,486 - H281DRAFT_04342 0.58 -0.9 61,488 - H281DRAFT_04342 0.59 -0.4 61,585 - H281DRAFT_04342 0.82 -1.4
Or see this region's nucleotide sequence