Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03726 and H281DRAFT_03727 are separated by 8 nucleotides H281DRAFT_03727 and H281DRAFT_03728 are separated by 33 nucleotides H281DRAFT_03728 and H281DRAFT_03729 are separated by 98 nucleotides
H281DRAFT_03726: H281DRAFT_03726 - Predicted dithiol-disulfide oxidoreductase, DUF899 family, at 241,012 to 241,779
_03726
H281DRAFT_03727: H281DRAFT_03727 - Predicted metal-binding membrane protein, at 241,788 to 242,633
_03727
H281DRAFT_03728: H281DRAFT_03728 - Uncharacterized conserved protein, DUF1697 family, at 242,667 to 243,176
_03728
H281DRAFT_03729: H281DRAFT_03729 - carbohydrate ABC transporter substrate-binding protein, CUT1 family, at 243,275 to 245,014
_03729
Position (kb)
242
243
244 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 241.697 kb on - strand, within H281DRAFT_03726 at 242.147 kb on + strand, within H281DRAFT_03727 at 242.412 kb on + strand, within H281DRAFT_03727 at 242.413 kb on - strand, within H281DRAFT_03727 at 242.425 kb on + strand, within H281DRAFT_03727 at 242.526 kb on - strand, within H281DRAFT_03727 at 243.070 kb on - strand, within H281DRAFT_03728 at 243.318 kb on + strand at 243.567 kb on + strand, within H281DRAFT_03729 at 243.621 kb on + strand, within H281DRAFT_03729 at 243.622 kb on - strand, within H281DRAFT_03729 at 243.852 kb on + strand, within H281DRAFT_03729 at 243.853 kb on - strand, within H281DRAFT_03729 at 244.151 kb on + strand, within H281DRAFT_03729 at 244.152 kb on - strand, within H281DRAFT_03729 at 244.152 kb on - strand, within H281DRAFT_03729
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 241,697 - H281DRAFT_03726 0.89 +1.6 242,147 + H281DRAFT_03727 0.42 -0.4 242,412 + H281DRAFT_03727 0.74 +0.5 242,413 - H281DRAFT_03727 0.74 -0.2 242,425 + H281DRAFT_03727 0.75 +0.2 242,526 - H281DRAFT_03727 0.87 -0.7 243,070 - H281DRAFT_03728 0.79 -0.3 243,318 + -1.0 243,567 + H281DRAFT_03729 0.17 +0.4 243,621 + H281DRAFT_03729 0.20 +0.5 243,622 - H281DRAFT_03729 0.20 +4.1 243,852 + H281DRAFT_03729 0.33 +0.3 243,853 - H281DRAFT_03729 0.33 +0.8 244,151 + H281DRAFT_03729 0.50 +1.2 244,152 - H281DRAFT_03729 0.50 -1.9 244,152 - H281DRAFT_03729 0.50 +0.1
Or see this region's nucleotide sequence