Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03605 and H281DRAFT_03606 overlap by 8 nucleotides H281DRAFT_03606 and H281DRAFT_03607 overlap by 4 nucleotides
H281DRAFT_03605: H281DRAFT_03605 - Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains, at 114,592 to 114,960
_03605
H281DRAFT_03606: H281DRAFT_03606 - CheC, inhibitor of MCP methylation, at 114,953 to 115,576
_03606
H281DRAFT_03607: H281DRAFT_03607 - PAS domain S-box-containing protein/diguanylate cyclase (GGDEF) domain-containing protein, at 115,573 to 116,511
_03607
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 113.984 kb on + strand at 113.985 kb on - strand at 113.985 kb on - strand at 114.013 kb on + strand at 114.042 kb on + strand at 114.043 kb on - strand at 114.043 kb on - strand at 114.043 kb on - strand at 114.043 kb on - strand at 114.043 kb on - strand at 114.058 kb on - strand at 114.088 kb on + strand at 114.165 kb on + strand at 114.166 kb on - strand at 114.192 kb on + strand at 114.193 kb on - strand at 114.250 kb on + strand at 114.293 kb on + strand at 114.369 kb on + strand at 114.370 kb on - strand at 114.395 kb on + strand at 114.396 kb on - strand at 114.396 kb on - strand at 114.396 kb on - strand at 114.396 kb on - strand at 114.495 kb on - strand at 114.495 kb on - strand at 114.571 kb on + strand at 114.571 kb on + strand at 114.572 kb on - strand at 114.572 kb on - strand at 114.685 kb on + strand, within H281DRAFT_03605 at 114.685 kb on + strand, within H281DRAFT_03605 at 114.685 kb on + strand, within H281DRAFT_03605 at 114.685 kb on + strand, within H281DRAFT_03605 at 114.718 kb on + strand, within H281DRAFT_03605 at 114.719 kb on - strand, within H281DRAFT_03605 at 114.779 kb on - strand, within H281DRAFT_03605 at 114.870 kb on - strand, within H281DRAFT_03605 at 114.924 kb on - strand at 114.943 kb on + strand at 114.944 kb on - strand at 114.952 kb on + strand at 114.952 kb on + strand at 114.952 kb on + strand at 114.952 kb on + strand at 114.952 kb on + strand at 114.952 kb on + strand at 114.952 kb on + strand at 114.952 kb on + strand at 114.952 kb on + strand at 114.953 kb on - strand at 114.953 kb on - strand at 114.953 kb on - strand at 114.953 kb on - strand at 114.953 kb on - strand at 114.953 kb on - strand at 115.068 kb on + strand, within H281DRAFT_03606 at 115.069 kb on - strand, within H281DRAFT_03606 at 115.136 kb on - strand, within H281DRAFT_03606 at 115.253 kb on + strand, within H281DRAFT_03606 at 115.254 kb on - strand, within H281DRAFT_03606 at 115.268 kb on + strand, within H281DRAFT_03606 at 115.584 kb on + strand at 115.677 kb on + strand, within H281DRAFT_03607 at 115.677 kb on + strand, within H281DRAFT_03607 at 115.678 kb on - strand, within H281DRAFT_03607 at 115.678 kb on - strand, within H281DRAFT_03607 at 115.678 kb on - strand, within H281DRAFT_03607 at 115.678 kb on - strand, within H281DRAFT_03607 at 115.678 kb on - strand, within H281DRAFT_03607 at 115.797 kb on + strand, within H281DRAFT_03607 at 115.797 kb on + strand, within H281DRAFT_03607 at 115.798 kb on - strand, within H281DRAFT_03607 at 115.831 kb on + strand, within H281DRAFT_03607 at 115.831 kb on + strand, within H281DRAFT_03607 at 115.831 kb on + strand, within H281DRAFT_03607 at 115.832 kb on - strand, within H281DRAFT_03607 at 115.832 kb on - strand, within H281DRAFT_03607 at 115.832 kb on - strand, within H281DRAFT_03607 at 115.879 kb on + strand, within H281DRAFT_03607 at 115.879 kb on + strand, within H281DRAFT_03607 at 115.879 kb on + strand, within H281DRAFT_03607 at 115.879 kb on + strand, within H281DRAFT_03607 at 116.018 kb on - strand, within H281DRAFT_03607 at 116.086 kb on + strand, within H281DRAFT_03607 at 116.086 kb on + strand, within H281DRAFT_03607 at 116.105 kb on - strand, within H281DRAFT_03607 at 116.428 kb on + strand at 116.429 kb on - strand at 116.429 kb on - strand at 116.464 kb on + strand at 116.509 kb on + strand at 116.509 kb on + strand at 116.509 kb on + strand at 116.536 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 113,984 + +0.4 113,985 - +3.0 113,985 - -1.3 114,013 + -1.2 114,042 + +0.4 114,043 - +0.1 114,043 - +0.3 114,043 - -0.7 114,043 - +0.3 114,043 - -2.1 114,058 - -0.1 114,088 + +0.4 114,165 + +0.6 114,166 - +1.3 114,192 + -0.3 114,193 - -0.3 114,250 + -0.2 114,293 + -1.4 114,369 + +1.0 114,370 - +0.9 114,395 + -0.9 114,396 - +0.6 114,396 - +0.7 114,396 - -0.5 114,396 - -0.7 114,495 - -1.9 114,495 - -0.2 114,571 + +0.0 114,571 + -2.1 114,572 - -0.9 114,572 - +0.9 114,685 + H281DRAFT_03605 0.25 +0.8 114,685 + H281DRAFT_03605 0.25 -0.5 114,685 + H281DRAFT_03605 0.25 -3.6 114,685 + H281DRAFT_03605 0.25 -0.7 114,718 + H281DRAFT_03605 0.34 -0.5 114,719 - H281DRAFT_03605 0.34 -0.1 114,779 - H281DRAFT_03605 0.51 -0.1 114,870 - H281DRAFT_03605 0.75 +0.3 114,924 - +0.1 114,943 + +0.1 114,944 - +0.3 114,952 + +1.7 114,952 + +0.4 114,952 + -0.1 114,952 + +0.1 114,952 + -1.2 114,952 + -1.3 114,952 + -0.4 114,952 + +0.4 114,952 + -0.5 114,953 - -1.8 114,953 - +0.2 114,953 - +0.9 114,953 - +0.1 114,953 - -0.4 114,953 - -0.6 115,068 + H281DRAFT_03606 0.18 -1.2 115,069 - H281DRAFT_03606 0.19 +0.8 115,136 - H281DRAFT_03606 0.29 +0.3 115,253 + H281DRAFT_03606 0.48 +0.9 115,254 - H281DRAFT_03606 0.48 +0.8 115,268 + H281DRAFT_03606 0.50 -0.4 115,584 + +0.6 115,677 + H281DRAFT_03607 0.11 +0.1 115,677 + H281DRAFT_03607 0.11 -0.0 115,678 - H281DRAFT_03607 0.11 +0.6 115,678 - H281DRAFT_03607 0.11 -0.4 115,678 - H281DRAFT_03607 0.11 -0.1 115,678 - H281DRAFT_03607 0.11 +0.5 115,678 - H281DRAFT_03607 0.11 -1.2 115,797 + H281DRAFT_03607 0.24 +0.9 115,797 + H281DRAFT_03607 0.24 +0.8 115,798 - H281DRAFT_03607 0.24 -0.9 115,831 + H281DRAFT_03607 0.27 +2.1 115,831 + H281DRAFT_03607 0.27 -0.1 115,831 + H281DRAFT_03607 0.27 -0.7 115,832 - H281DRAFT_03607 0.28 -0.9 115,832 - H281DRAFT_03607 0.28 -2.3 115,832 - H281DRAFT_03607 0.28 +0.0 115,879 + H281DRAFT_03607 0.33 +1.5 115,879 + H281DRAFT_03607 0.33 +0.8 115,879 + H281DRAFT_03607 0.33 +0.1 115,879 + H281DRAFT_03607 0.33 +2.5 116,018 - H281DRAFT_03607 0.47 -1.2 116,086 + H281DRAFT_03607 0.55 -0.1 116,086 + H281DRAFT_03607 0.55 +0.2 116,105 - H281DRAFT_03607 0.57 -0.1 116,428 + +0.6 116,429 - +0.3 116,429 - +0.7 116,464 + +0.2 116,509 + +0.3 116,509 + +0.2 116,509 + +0.5 116,536 + -0.2
Or see this region's nucleotide sequence