Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03575 and H281DRAFT_03576 are separated by 663 nucleotides H281DRAFT_03576 and H281DRAFT_03577 are separated by 28 nucleotides H281DRAFT_03577 and H281DRAFT_03578 overlap by 4 nucleotides
H281DRAFT_03575: H281DRAFT_03575 - hypothetical protein, at 83,345 to 84,103
_03575
H281DRAFT_03576: H281DRAFT_03576 - two-component system, chemotaxis family, response regulator CheY, at 84,767 to 85,132
_03576
H281DRAFT_03577: H281DRAFT_03577 - hypothetical protein, at 85,161 to 85,700
_03577
H281DRAFT_03578: H281DRAFT_03578 - methyl-accepting chemotaxis sensory transducer, at 85,697 to 86,431
_03578
Position (kb) 84
85
86 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 83.808 kb on - strand, within H281DRAFT_03575 at 84.036 kb on - strand at 84.043 kb on + strand at 84.044 kb on - strand at 84.207 kb on - strand at 84.207 kb on - strand at 84.380 kb on + strand at 84.380 kb on + strand at 84.465 kb on + strand at 84.524 kb on + strand at 84.583 kb on - strand at 84.699 kb on - strand at 84.944 kb on - strand, within H281DRAFT_03576 at 85.004 kb on - strand, within H281DRAFT_03576 at 85.115 kb on + strand at 85.132 kb on + strand at 85.133 kb on - strand at 85.133 kb on - strand at 85.289 kb on - strand at 85.351 kb on + strand, within H281DRAFT_03577 at 85.352 kb on - strand, within H281DRAFT_03577 at 85.381 kb on + strand, within H281DRAFT_03577 at 85.382 kb on - strand, within H281DRAFT_03577 at 85.552 kb on - strand, within H281DRAFT_03577 at 85.663 kb on - strand at 85.846 kb on - strand, within H281DRAFT_03578
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 83,808 - H281DRAFT_03575 0.61 -1.9 84,036 - -1.4 84,043 + -0.6 84,044 - +0.3 84,207 - +2.0 84,207 - -0.9 84,380 + +2.1 84,380 + +1.0 84,465 + -0.2 84,524 + -0.3 84,583 - -2.4 84,699 - -0.5 84,944 - H281DRAFT_03576 0.48 +0.7 85,004 - H281DRAFT_03576 0.65 -2.6 85,115 + +0.4 85,132 + +1.2 85,133 - -0.9 85,133 - +0.9 85,289 - +4.8 85,351 + H281DRAFT_03577 0.35 -0.6 85,352 - H281DRAFT_03577 0.35 +0.4 85,381 + H281DRAFT_03577 0.41 +1.1 85,382 - H281DRAFT_03577 0.41 +0.1 85,552 - H281DRAFT_03577 0.72 +1.6 85,663 - +0.4 85,846 - H281DRAFT_03578 0.20 +1.1
Or see this region's nucleotide sequence