Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03370 and H281DRAFT_03371 overlap by 4 nucleotides H281DRAFT_03371 and H281DRAFT_03372 are separated by 18 nucleotides H281DRAFT_03372 and H281DRAFT_03373 are separated by 87 nucleotides
H281DRAFT_03370: H281DRAFT_03370 - multidrug resistance protein MdtO, at 431,396 to 433,504
_03370
H281DRAFT_03371: H281DRAFT_03371 - outer membrane protein, multidrug efflux system, at 433,501 to 434,988
_03371
H281DRAFT_03372: H281DRAFT_03372 - hypothetical protein, at 435,007 to 435,615
_03372
H281DRAFT_03373: H281DRAFT_03373 - Low temperature requirement protein LtrA, at 435,703 to 436,815
_03373
Position (kb) 433
434
435 Strain fitness (log2 ratio) -2
-1
0
1 at 432.521 kb on + strand, within H281DRAFT_03370 at 432.530 kb on + strand, within H281DRAFT_03370 at 432.783 kb on - strand, within H281DRAFT_03370 at 432.783 kb on - strand, within H281DRAFT_03370 at 433.148 kb on - strand, within H281DRAFT_03370 at 434.116 kb on + strand, within H281DRAFT_03371 at 434.116 kb on + strand, within H281DRAFT_03371 at 434.675 kb on - strand, within H281DRAFT_03371 at 435.442 kb on - strand, within H281DRAFT_03372 at 435.496 kb on - strand, within H281DRAFT_03372
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 432,521 + H281DRAFT_03370 0.53 -0.2 432,530 + H281DRAFT_03370 0.54 -0.9 432,783 - H281DRAFT_03370 0.66 +1.6 432,783 - H281DRAFT_03370 0.66 -1.1 433,148 - H281DRAFT_03370 0.83 +0.3 434,116 + H281DRAFT_03371 0.41 +0.1 434,116 + H281DRAFT_03371 0.41 -0.5 434,675 - H281DRAFT_03371 0.79 +0.9 435,442 - H281DRAFT_03372 0.71 -1.3 435,496 - H281DRAFT_03372 0.80 -1.9
Or see this region's nucleotide sequence