Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03342 and H281DRAFT_03343 are separated by 254 nucleotides H281DRAFT_03343 and H281DRAFT_03344 are separated by 61 nucleotides
H281DRAFT_03342: H281DRAFT_03342 - Glyoxylase, beta-lactamase superfamily II, at 400,415 to 401,290
_03342
H281DRAFT_03343: H281DRAFT_03343 - Membrane-bound lytic murein transglycosylase MltF, at 401,545 to 403,167
_03343
H281DRAFT_03344: H281DRAFT_03344 - Predicted ester cyclase, at 403,229 to 403,807
_03344
Position (kb) 401
402
403
404 Strain fitness (log2 ratio) -2
-1
0
1
2 at 401.473 kb on + strand at 401.525 kb on + strand at 401.645 kb on - strand at 401.897 kb on - strand, within H281DRAFT_03343 at 401.974 kb on + strand at 402.248 kb on - strand, within H281DRAFT_03343 at 402.248 kb on - strand, within H281DRAFT_03343 at 402.581 kb on - strand, within H281DRAFT_03343 at 402.581 kb on - strand, within H281DRAFT_03343 at 402.582 kb on + strand, within H281DRAFT_03343 at 402.582 kb on + strand, within H281DRAFT_03343 at 402.583 kb on - strand, within H281DRAFT_03343 at 402.583 kb on - strand, within H281DRAFT_03343 at 402.583 kb on - strand, within H281DRAFT_03343 at 402.606 kb on + strand, within H281DRAFT_03343 at 402.668 kb on - strand, within H281DRAFT_03343 at 402.795 kb on + strand, within H281DRAFT_03343 at 402.856 kb on + strand, within H281DRAFT_03343 at 403.066 kb on + strand at 403.082 kb on - strand at 403.230 kb on + strand at 403.317 kb on + strand, within H281DRAFT_03344 at 403.400 kb on + strand, within H281DRAFT_03344 at 403.600 kb on + strand, within H281DRAFT_03344 at 403.606 kb on - strand, within H281DRAFT_03344 at 403.913 kb on + strand at 403.914 kb on - strand at 404.120 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 401,473 + -0.3 401,525 + +0.9 401,645 - -1.7 401,897 - H281DRAFT_03343 0.22 +1.5 401,974 + +0.9 402,248 - H281DRAFT_03343 0.43 +0.4 402,248 - H281DRAFT_03343 0.43 +0.1 402,581 - H281DRAFT_03343 0.64 +1.4 402,581 - H281DRAFT_03343 0.64 -1.1 402,582 + H281DRAFT_03343 0.64 -0.3 402,582 + H281DRAFT_03343 0.64 -1.5 402,583 - H281DRAFT_03343 0.64 -2.1 402,583 - H281DRAFT_03343 0.64 +0.3 402,583 - H281DRAFT_03343 0.64 -0.0 402,606 + H281DRAFT_03343 0.65 +1.8 402,668 - H281DRAFT_03343 0.69 -1.0 402,795 + H281DRAFT_03343 0.77 -0.2 402,856 + H281DRAFT_03343 0.81 -1.9 403,066 + -0.3 403,082 - -1.5 403,230 + -0.2 403,317 + H281DRAFT_03344 0.15 +1.1 403,400 + H281DRAFT_03344 0.30 +0.2 403,600 + H281DRAFT_03344 0.64 -0.2 403,606 - H281DRAFT_03344 0.65 -0.0 403,913 + +0.2 403,914 - -0.8 404,120 + +0.2
Or see this region's nucleotide sequence