Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03119 and H281DRAFT_03120 are separated by 23 nucleotides H281DRAFT_03120 and H281DRAFT_03121 are separated by 27 nucleotides H281DRAFT_03121 and H281DRAFT_03122 overlap by 8 nucleotides
H281DRAFT_03119: H281DRAFT_03119 - NADH dehydrogenase/NADH:ubiquinone oxidoreductase 75 kD subunit (chain G), at 152,634 to 153,002
_03119
H281DRAFT_03120: H281DRAFT_03120 - putative spermidine/putrescine transport system permease protein, at 153,026 to 153,820
_03120
H281DRAFT_03121: H281DRAFT_03121 - putative spermidine/putrescine transport system permease protein, at 153,848 to 154,762
_03121
H281DRAFT_03122: H281DRAFT_03122 - putative spermidine/putrescine transport system ATP-binding protein, at 154,755 to 155,846
_03122
Position (kb) 153
154
155 Strain fitness (log2 ratio) -2
-1
0
1
2 at 153.158 kb on - strand, within H281DRAFT_03120 at 153.158 kb on - strand, within H281DRAFT_03120 at 153.160 kb on - strand, within H281DRAFT_03120 at 153.397 kb on + strand, within H281DRAFT_03120 at 153.867 kb on + strand at 153.867 kb on + strand at 153.891 kb on + strand at 154.260 kb on + strand at 154.435 kb on - strand at 154.519 kb on - strand, within H281DRAFT_03121 at 154.870 kb on - strand, within H281DRAFT_03122 at 155.013 kb on + strand, within H281DRAFT_03122 at 155.183 kb on - strand, within H281DRAFT_03122 at 155.191 kb on + strand at 155.192 kb on - strand at 155.639 kb on + strand, within H281DRAFT_03122 at 155.640 kb on - strand, within H281DRAFT_03122
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 153,158 - H281DRAFT_03120 0.17 -0.4 153,158 - H281DRAFT_03120 0.17 -1.9 153,160 - H281DRAFT_03120 0.17 +0.2 153,397 + H281DRAFT_03120 0.47 +0.6 153,867 + -0.5 153,867 + -0.9 153,891 + -0.0 154,260 + +2.2 154,435 - -0.0 154,519 - H281DRAFT_03121 0.73 -2.0 154,870 - H281DRAFT_03122 0.11 +0.5 155,013 + H281DRAFT_03122 0.24 +0.5 155,183 - H281DRAFT_03122 0.39 +0.1 155,191 + -1.4 155,192 - -1.0 155,639 + H281DRAFT_03122 0.81 +0.8 155,640 - H281DRAFT_03122 0.81 -1.8
Or see this region's nucleotide sequence