Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02917 and H281DRAFT_02918 are separated by 245 nucleotides H281DRAFT_02918 and H281DRAFT_02919 are separated by 220 nucleotides H281DRAFT_02919 and H281DRAFT_02920 are separated by 110 nucleotides
H281DRAFT_02917: H281DRAFT_02917 - DNA topoisomerase-1, at 523,213 to 524,565
_02917
H281DRAFT_02918: H281DRAFT_02918 - Uncharacterized protein conserved in bacteria, at 524,811 to 525,326
_02918
H281DRAFT_02919: H281DRAFT_02919 - Protein of unknown function (DUF3175), at 525,547 to 525,966
_02919
H281DRAFT_02920: H281DRAFT_02920 - sodium/proton antiporter, CPA1 family, at 526,077 to 527,396
_02920
Position (kb) 525
526 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 524.575 kb on + strand at 524.576 kb on - strand at 524.852 kb on + strand at 524.853 kb on - strand at 525.226 kb on - strand, within H281DRAFT_02918 at 525.263 kb on - strand, within H281DRAFT_02918 at 525.275 kb on + strand at 525.324 kb on + strand at 525.325 kb on - strand at 525.530 kb on - strand at 525.868 kb on + strand, within H281DRAFT_02919 at 526.179 kb on + strand at 526.180 kb on - strand at 526.180 kb on - strand at 526.676 kb on - strand, within H281DRAFT_02920 at 526.703 kb on - strand, within H281DRAFT_02920
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 524,575 + -3.0 524,576 - +3.1 524,852 + +0.1 524,853 - -0.0 525,226 - H281DRAFT_02918 0.80 -0.3 525,263 - H281DRAFT_02918 0.88 +0.5 525,275 + -0.6 525,324 + +0.6 525,325 - -1.3 525,530 - -1.3 525,868 + H281DRAFT_02919 0.76 -0.3 526,179 + -0.1 526,180 - -1.2 526,180 - -0.4 526,676 - H281DRAFT_02920 0.45 -0.2 526,703 - H281DRAFT_02920 0.47 +0.0
Or see this region's nucleotide sequence