Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02858 and H281DRAFT_02859 are separated by 29 nucleotides H281DRAFT_02859 and H281DRAFT_02860 overlap by 4 nucleotides H281DRAFT_02860 and H281DRAFT_02861 are separated by 5 nucleotides
H281DRAFT_02858: H281DRAFT_02858 - precorrin-4 C11-methyltransferase /precorrin-2 C20-methyltransferase, at 451,966 to 452,691
_02858
H281DRAFT_02859: H281DRAFT_02859 - precorrin-6A reductase (EC 1.3.1.54), at 452,721 to 453,443
_02859
H281DRAFT_02860: H281DRAFT_02860 - cobalt-precorrin-5B (C1)-methyltransferase, at 453,440 to 454,528
_02860
H281DRAFT_02861: H281DRAFT_02861 - precorrin-6Y C5,15-methyltransferase (decarboxylating), at 454,534 to 455,793
_02861
Position (kb) 453
454
455 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 453.275 kb on + strand, within H281DRAFT_02859 at 453.276 kb on - strand, within H281DRAFT_02859 at 453.277 kb on + strand, within H281DRAFT_02859 at 453.278 kb on - strand, within H281DRAFT_02859 at 453.367 kb on + strand, within H281DRAFT_02859 at 453.383 kb on + strand at 453.383 kb on + strand at 453.402 kb on + strand at 453.402 kb on + strand at 453.403 kb on - strand at 453.701 kb on - strand, within H281DRAFT_02860 at 453.787 kb on + strand, within H281DRAFT_02860 at 453.886 kb on + strand, within H281DRAFT_02860 at 453.887 kb on - strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.905 kb on - strand, within H281DRAFT_02860 at 453.905 kb on - strand, within H281DRAFT_02860 at 453.906 kb on + strand, within H281DRAFT_02860 at 454.098 kb on + strand, within H281DRAFT_02860 at 454.572 kb on + strand at 454.603 kb on + strand at 454.603 kb on + strand at 454.603 kb on + strand at 454.610 kb on + strand at 454.610 kb on + strand at 454.610 kb on + strand at 454.611 kb on - strand at 454.611 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 453,275 + H281DRAFT_02859 0.77 -1.4 453,276 - H281DRAFT_02859 0.77 -3.2 453,277 + H281DRAFT_02859 0.77 -0.1 453,278 - H281DRAFT_02859 0.77 -3.6 453,367 + H281DRAFT_02859 0.89 +0.6 453,383 + -0.5 453,383 + +0.5 453,402 + -1.6 453,402 + -2.4 453,403 - -0.5 453,701 - H281DRAFT_02860 0.24 +0.0 453,787 + H281DRAFT_02860 0.32 -1.6 453,886 + H281DRAFT_02860 0.41 -0.3 453,887 - H281DRAFT_02860 0.41 +0.2 453,904 + H281DRAFT_02860 0.43 +1.7 453,904 + H281DRAFT_02860 0.43 -0.2 453,904 + H281DRAFT_02860 0.43 -0.6 453,904 + H281DRAFT_02860 0.43 -0.2 453,904 + H281DRAFT_02860 0.43 +0.5 453,904 + H281DRAFT_02860 0.43 -0.4 453,905 - H281DRAFT_02860 0.43 +0.2 453,905 - H281DRAFT_02860 0.43 -1.6 453,906 + H281DRAFT_02860 0.43 -1.3 454,098 + H281DRAFT_02860 0.60 -0.4 454,572 + +0.7 454,603 + -1.3 454,603 + -0.2 454,603 + -0.6 454,610 + -0.8 454,610 + +0.5 454,610 + -0.3 454,611 - -0.3 454,611 - -0.7
Or see this region's nucleotide sequence