Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02710 and H281DRAFT_02711 overlap by 26 nucleotides H281DRAFT_02711 and H281DRAFT_02712 are separated by 29 nucleotides H281DRAFT_02712 and H281DRAFT_02713 overlap by 1 nucleotides H281DRAFT_02713 and H281DRAFT_02714 are separated by 39 nucleotides
H281DRAFT_02710: H281DRAFT_02710 - transcriptional regulator, Crp/Fnr family, at 293,857 to 294,615
_02710
H281DRAFT_02711: H281DRAFT_02711 - Hydantoin racemase, at 294,590 to 295,246
_02711
H281DRAFT_02712: H281DRAFT_02712 - monosaccharide ABC transporter ATP-binding protein, CUT2 family, at 295,276 to 296,793
_02712
H281DRAFT_02713: H281DRAFT_02713 - monosaccharide ABC transporter substrate-binding protein, CUT2 family, at 296,793 to 297,734
_02713
H281DRAFT_02714: H281DRAFT_02714 - monosaccharide ABC transporter membrane protein, CUT2 family, at 297,774 to 298,769
_02714
Position (kb) 295
296
297 Strain fitness (log2 ratio) -2
-1
0
1
2 at 294.314 kb on + strand, within H281DRAFT_02710 at 294.314 kb on + strand, within H281DRAFT_02710 at 294.315 kb on - strand, within H281DRAFT_02710 at 294.315 kb on - strand, within H281DRAFT_02710 at 294.533 kb on - strand, within H281DRAFT_02710 at 295.071 kb on + strand, within H281DRAFT_02711 at 295.071 kb on + strand, within H281DRAFT_02711 at 295.071 kb on + strand, within H281DRAFT_02711 at 295.071 kb on + strand, within H281DRAFT_02711 at 295.072 kb on - strand, within H281DRAFT_02711 at 295.072 kb on - strand, within H281DRAFT_02711 at 295.072 kb on - strand, within H281DRAFT_02711 at 295.072 kb on - strand, within H281DRAFT_02711 at 295.075 kb on + strand, within H281DRAFT_02711 at 295.077 kb on + strand, within H281DRAFT_02711 at 295.078 kb on - strand, within H281DRAFT_02711 at 295.078 kb on - strand, within H281DRAFT_02711 at 295.150 kb on + strand, within H281DRAFT_02711 at 295.150 kb on + strand, within H281DRAFT_02711 at 295.226 kb on + strand at 295.226 kb on + strand at 295.226 kb on + strand at 295.227 kb on - strand at 295.227 kb on - strand at 295.246 kb on + strand at 295.475 kb on - strand, within H281DRAFT_02712 at 295.679 kb on + strand, within H281DRAFT_02712 at 295.679 kb on + strand, within H281DRAFT_02712 at 295.695 kb on + strand, within H281DRAFT_02712 at 295.696 kb on - strand, within H281DRAFT_02712 at 295.769 kb on - strand, within H281DRAFT_02712 at 295.822 kb on + strand, within H281DRAFT_02712 at 295.913 kb on - strand, within H281DRAFT_02712 at 295.939 kb on + strand, within H281DRAFT_02712 at 295.940 kb on - strand, within H281DRAFT_02712 at 295.941 kb on + strand, within H281DRAFT_02712 at 295.941 kb on + strand, within H281DRAFT_02712 at 295.942 kb on - strand, within H281DRAFT_02712 at 296.187 kb on + strand, within H281DRAFT_02712 at 296.188 kb on - strand, within H281DRAFT_02712 at 296.286 kb on + strand, within H281DRAFT_02712 at 296.350 kb on + strand, within H281DRAFT_02712 at 296.421 kb on - strand, within H281DRAFT_02712 at 296.523 kb on - strand, within H281DRAFT_02712 at 296.618 kb on + strand, within H281DRAFT_02712 at 296.783 kb on - strand at 296.783 kb on - strand at 296.804 kb on - strand at 296.814 kb on - strand at 296.814 kb on - strand at 296.950 kb on + strand, within H281DRAFT_02713 at 296.950 kb on + strand, within H281DRAFT_02713 at 296.951 kb on - strand, within H281DRAFT_02713 at 297.350 kb on - strand, within H281DRAFT_02713 at 297.350 kb on - strand, within H281DRAFT_02713 at 297.350 kb on - strand, within H281DRAFT_02713 at 297.350 kb on - strand, within H281DRAFT_02713 at 297.350 kb on - strand, within H281DRAFT_02713 at 297.350 kb on - strand, within H281DRAFT_02713 at 297.508 kb on - strand, within H281DRAFT_02713 at 297.645 kb on - strand at 297.775 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 294,314 + H281DRAFT_02710 0.60 -0.6 294,314 + H281DRAFT_02710 0.60 -0.5 294,315 - H281DRAFT_02710 0.60 -1.3 294,315 - H281DRAFT_02710 0.60 -0.9 294,533 - H281DRAFT_02710 0.89 -0.6 295,071 + H281DRAFT_02711 0.73 +0.4 295,071 + H281DRAFT_02711 0.73 +0.2 295,071 + H281DRAFT_02711 0.73 +0.2 295,071 + H281DRAFT_02711 0.73 +2.1 295,072 - H281DRAFT_02711 0.73 +0.9 295,072 - H281DRAFT_02711 0.73 -1.2 295,072 - H281DRAFT_02711 0.73 +0.5 295,072 - H281DRAFT_02711 0.73 -0.2 295,075 + H281DRAFT_02711 0.74 -0.8 295,077 + H281DRAFT_02711 0.74 +0.4 295,078 - H281DRAFT_02711 0.74 +0.6 295,078 - H281DRAFT_02711 0.74 +1.2 295,150 + H281DRAFT_02711 0.85 -1.9 295,150 + H281DRAFT_02711 0.85 -1.6 295,226 + -0.1 295,226 + -1.0 295,226 + +0.6 295,227 - -0.2 295,227 - -1.3 295,246 + -0.0 295,475 - H281DRAFT_02712 0.13 -0.1 295,679 + H281DRAFT_02712 0.27 +0.2 295,679 + H281DRAFT_02712 0.27 -0.1 295,695 + H281DRAFT_02712 0.28 +0.2 295,696 - H281DRAFT_02712 0.28 -0.8 295,769 - H281DRAFT_02712 0.32 +0.3 295,822 + H281DRAFT_02712 0.36 +0.0 295,913 - H281DRAFT_02712 0.42 -0.9 295,939 + H281DRAFT_02712 0.44 +0.3 295,940 - H281DRAFT_02712 0.44 -0.9 295,941 + H281DRAFT_02712 0.44 -0.1 295,941 + H281DRAFT_02712 0.44 +1.1 295,942 - H281DRAFT_02712 0.44 -0.5 296,187 + H281DRAFT_02712 0.60 -0.6 296,188 - H281DRAFT_02712 0.60 +0.0 296,286 + H281DRAFT_02712 0.67 +0.9 296,350 + H281DRAFT_02712 0.71 -0.5 296,421 - H281DRAFT_02712 0.75 +0.3 296,523 - H281DRAFT_02712 0.82 +1.2 296,618 + H281DRAFT_02712 0.88 +0.2 296,783 - -0.3 296,783 - -0.3 296,804 - -1.4 296,814 - -0.1 296,814 - +1.6 296,950 + H281DRAFT_02713 0.17 -0.4 296,950 + H281DRAFT_02713 0.17 +0.8 296,951 - H281DRAFT_02713 0.17 +0.7 297,350 - H281DRAFT_02713 0.59 +0.4 297,350 - H281DRAFT_02713 0.59 +0.7 297,350 - H281DRAFT_02713 0.59 +1.2 297,350 - H281DRAFT_02713 0.59 +0.3 297,350 - H281DRAFT_02713 0.59 +0.6 297,350 - H281DRAFT_02713 0.59 +0.4 297,508 - H281DRAFT_02713 0.76 -0.7 297,645 - +0.9 297,775 + -0.7
Or see this region's nucleotide sequence