Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02358 and H281DRAFT_02359 are separated by 139 nucleotides H281DRAFT_02359 and H281DRAFT_02360 are separated by 413 nucleotides
H281DRAFT_02358: H281DRAFT_02358 - GTPase, G3E family, at 517,084 to 518,400
_02358
H281DRAFT_02359: H281DRAFT_02359 - HU family DNA-binding protein, at 518,540 to 518,818
_02359
H281DRAFT_02360: H281DRAFT_02360 - tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein, at 519,232 to 521,211
_02360
Position (kb) 518
519 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 517.848 kb on + strand, within H281DRAFT_02358 at 517.848 kb on + strand, within H281DRAFT_02358 at 517.849 kb on - strand, within H281DRAFT_02358 at 517.849 kb on - strand, within H281DRAFT_02358 at 518.268 kb on - strand, within H281DRAFT_02358 at 518.412 kb on + strand at 518.413 kb on - strand at 518.416 kb on - strand at 518.533 kb on - strand at 518.819 kb on + strand at 518.820 kb on - strand at 518.838 kb on + strand at 518.838 kb on + strand at 518.839 kb on - strand at 518.901 kb on + strand at 518.901 kb on + strand at 518.985 kb on + strand at 518.985 kb on + strand at 518.985 kb on + strand at 518.986 kb on - strand at 519.051 kb on + strand at 519.052 kb on - strand at 519.068 kb on - strand at 519.245 kb on + strand at 519.299 kb on - strand at 519.306 kb on + strand at 519.306 kb on + strand at 519.306 kb on + strand at 519.307 kb on - strand at 519.307 kb on - strand at 519.310 kb on + strand at 519.310 kb on + strand at 519.310 kb on + strand at 519.310 kb on + strand at 519.311 kb on - strand at 519.311 kb on - strand at 519.311 kb on - strand at 519.357 kb on - strand at 519.357 kb on - strand at 519.357 kb on - strand at 519.547 kb on + strand, within H281DRAFT_02360 at 519.659 kb on + strand, within H281DRAFT_02360 at 519.659 kb on + strand, within H281DRAFT_02360 at 519.659 kb on + strand, within H281DRAFT_02360 at 519.660 kb on - strand, within H281DRAFT_02360 at 519.671 kb on + strand, within H281DRAFT_02360 at 519.671 kb on + strand, within H281DRAFT_02360 at 519.671 kb on + strand, within H281DRAFT_02360 at 519.671 kb on + strand, within H281DRAFT_02360 at 519.672 kb on - strand, within H281DRAFT_02360 at 519.731 kb on - strand, within H281DRAFT_02360 at 519.731 kb on - strand, within H281DRAFT_02360
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 517,848 + H281DRAFT_02358 0.58 -2.4 517,848 + H281DRAFT_02358 0.58 -2.5 517,849 - H281DRAFT_02358 0.58 -3.6 517,849 - H281DRAFT_02358 0.58 -1.9 518,268 - H281DRAFT_02358 0.90 -2.6 518,412 + +0.0 518,413 - -1.7 518,416 - -0.4 518,533 - -1.0 518,819 + -1.9 518,820 - -1.7 518,838 + +1.3 518,838 + -0.1 518,839 - -0.0 518,901 + +2.1 518,901 + -1.1 518,985 + -1.6 518,985 + -1.2 518,985 + -1.5 518,986 - +1.1 519,051 + +0.4 519,052 - -0.4 519,068 - +0.5 519,245 + -0.4 519,299 - +0.1 519,306 + -0.8 519,306 + -0.1 519,306 + -3.4 519,307 - -0.0 519,307 - -0.6 519,310 + -0.2 519,310 + -0.9 519,310 + -2.5 519,310 + -1.0 519,311 - +0.1 519,311 - -0.9 519,311 - -0.7 519,357 - +0.1 519,357 - -0.9 519,357 - +0.4 519,547 + H281DRAFT_02360 0.16 -0.8 519,659 + H281DRAFT_02360 0.22 +0.1 519,659 + H281DRAFT_02360 0.22 +0.7 519,659 + H281DRAFT_02360 0.22 +0.6 519,660 - H281DRAFT_02360 0.22 -2.8 519,671 + H281DRAFT_02360 0.22 +1.2 519,671 + H281DRAFT_02360 0.22 -1.6 519,671 + H281DRAFT_02360 0.22 +1.8 519,671 + H281DRAFT_02360 0.22 +2.1 519,672 - H281DRAFT_02360 0.22 -0.4 519,731 - H281DRAFT_02360 0.25 -0.3 519,731 - H281DRAFT_02360 0.25 +0.0
Or see this region's nucleotide sequence