Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02230 and H281DRAFT_02231 are separated by 6 nucleotides H281DRAFT_02231 and H281DRAFT_02232 are separated by 72 nucleotides H281DRAFT_02232 and H281DRAFT_02233 are separated by 380 nucleotides H281DRAFT_02233 and H281DRAFT_02234 are separated by 95 nucleotides
H281DRAFT_02230: H281DRAFT_02230 - 6-pyruvoyltetrahydropterin/6-carboxytetrahydropterin synthase, at 375,467 to 375,913
_02230
H281DRAFT_02231: H281DRAFT_02231 - 7-carboxy-7-deazaguanine synthase, Cx14CxxC type, at 375,920 to 376,552
_02231
H281DRAFT_02232: H281DRAFT_02232 - preQ(0) biosynthesis protein QueC, at 376,625 to 377,356
_02232
H281DRAFT_02233: H281DRAFT_02233 - tRNA_Phe_GAA, at 377,737 to 377,812
_02233
H281DRAFT_02234: H281DRAFT_02234 - two component transcriptional regulator, Fis family, at 377,908 to 378,600
_02234
Position (kb) 376
377
378 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 375.822 kb on + strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.842 kb on - strand, within H281DRAFT_02230 at 375.936 kb on + strand at 375.936 kb on + strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 376.048 kb on - strand, within H281DRAFT_02231 at 376.244 kb on + strand, within H281DRAFT_02231 at 376.244 kb on + strand, within H281DRAFT_02231 at 376.522 kb on + strand at 376.522 kb on + strand at 376.522 kb on + strand at 376.523 kb on - strand at 376.523 kb on - strand at 376.524 kb on + strand at 376.525 kb on - strand at 376.574 kb on + strand at 376.749 kb on + strand, within H281DRAFT_02232 at 376.750 kb on - strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.766 kb on + strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.799 kb on + strand, within H281DRAFT_02232 at 376.800 kb on - strand, within H281DRAFT_02232 at 376.822 kb on + strand, within H281DRAFT_02232 at 377.045 kb on - strand, within H281DRAFT_02232 at 377.173 kb on + strand, within H281DRAFT_02232 at 377.173 kb on + strand at 377.180 kb on + strand, within H281DRAFT_02232 at 377.235 kb on + strand, within H281DRAFT_02232 at 377.235 kb on + strand, within H281DRAFT_02232 at 377.236 kb on - strand, within H281DRAFT_02232 at 377.266 kb on - strand, within H281DRAFT_02232 at 377.460 kb on + strand at 377.460 kb on + strand at 377.461 kb on - strand at 377.461 kb on - strand at 377.461 kb on - strand at 377.462 kb on + strand at 377.463 kb on - strand at 377.499 kb on + strand at 377.499 kb on + strand at 377.500 kb on - strand at 377.500 kb on - strand at 377.500 kb on - strand at 377.500 kb on - strand at 377.501 kb on + strand at 377.502 kb on - strand at 377.614 kb on + strand at 377.653 kb on + strand at 377.653 kb on + strand at 377.654 kb on - strand at 377.656 kb on + strand at 377.657 kb on - strand at 377.657 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 375,822 + H281DRAFT_02230 0.79 +0.5 375,823 - H281DRAFT_02230 0.80 -0.6 375,823 - H281DRAFT_02230 0.80 -0.1 375,823 - H281DRAFT_02230 0.80 -1.0 375,823 - H281DRAFT_02230 0.80 +0.6 375,842 - H281DRAFT_02230 0.84 +1.9 375,936 + -2.0 375,936 + +0.4 375,937 - -0.5 375,937 - -0.8 375,937 - -0.5 375,937 - -0.8 375,937 - +0.7 376,048 - H281DRAFT_02231 0.20 -2.3 376,244 + H281DRAFT_02231 0.51 -1.1 376,244 + H281DRAFT_02231 0.51 +1.0 376,522 + -0.6 376,522 + -0.5 376,522 + -0.6 376,523 - +1.0 376,523 - -0.4 376,524 + +0.8 376,525 - +1.3 376,574 + -0.1 376,749 + H281DRAFT_02232 0.17 +1.9 376,750 - H281DRAFT_02232 0.17 +0.2 376,756 + H281DRAFT_02232 0.18 +0.2 376,756 + H281DRAFT_02232 0.18 +1.5 376,756 + H281DRAFT_02232 0.18 -0.5 376,766 + H281DRAFT_02232 0.19 -0.0 376,767 - H281DRAFT_02232 0.19 -0.5 376,767 - H281DRAFT_02232 0.19 +0.5 376,767 - H281DRAFT_02232 0.19 +0.5 376,767 - H281DRAFT_02232 0.19 +0.1 376,799 + H281DRAFT_02232 0.24 -0.8 376,800 - H281DRAFT_02232 0.24 +3.9 376,822 + H281DRAFT_02232 0.27 -0.5 377,045 - H281DRAFT_02232 0.57 -1.3 377,173 + H281DRAFT_02232 0.75 -0.5 377,173 + +2.1 377,180 + H281DRAFT_02232 0.76 +0.9 377,235 + H281DRAFT_02232 0.83 -0.8 377,235 + H281DRAFT_02232 0.83 +1.7 377,236 - H281DRAFT_02232 0.83 -1.0 377,266 - H281DRAFT_02232 0.88 -2.6 377,460 + +1.0 377,460 + +1.5 377,461 - -1.0 377,461 - -1.2 377,461 - +0.2 377,462 + +0.2 377,463 - +0.9 377,499 + +1.4 377,499 + -1.2 377,500 - +0.4 377,500 - +1.5 377,500 - -0.9 377,500 - -0.2 377,501 + -0.3 377,502 - +0.1 377,614 + -0.8 377,653 + -0.5 377,653 + +1.1 377,654 - -0.7 377,656 + +0.2 377,657 - -0.6 377,657 - -0.8
Or see this region's nucleotide sequence