Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02099 and H281DRAFT_02100 are separated by 309 nucleotides H281DRAFT_02100 and H281DRAFT_02101 are separated by 76 nucleotides
H281DRAFT_02099: H281DRAFT_02099 - hypothetical protein, at 226,041 to 226,643
_02099
H281DRAFT_02100: H281DRAFT_02100 - glyoxylate/hydroxypyruvate reductase A, at 226,953 to 227,894
_02100
H281DRAFT_02101: H281DRAFT_02101 - hydroxymethylglutaryl-CoA lyase, at 227,971 to 228,897
_02101
Position (kb) 226
227
228 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 225.994 kb on + strand at 225.994 kb on + strand at 226.421 kb on + strand, within H281DRAFT_02099 at 226.422 kb on - strand, within H281DRAFT_02099 at 226.824 kb on - strand at 226.829 kb on + strand at 226.829 kb on + strand at 226.969 kb on - strand at 227.083 kb on + strand, within H281DRAFT_02100 at 227.084 kb on - strand, within H281DRAFT_02100 at 227.260 kb on - strand, within H281DRAFT_02100 at 227.264 kb on - strand, within H281DRAFT_02100 at 227.264 kb on - strand, within H281DRAFT_02100 at 227.264 kb on - strand, within H281DRAFT_02100 at 227.283 kb on + strand, within H281DRAFT_02100 at 227.284 kb on - strand, within H281DRAFT_02100 at 227.304 kb on + strand, within H281DRAFT_02100 at 227.339 kb on - strand, within H281DRAFT_02100 at 227.339 kb on - strand, within H281DRAFT_02100 at 227.449 kb on - strand, within H281DRAFT_02100 at 227.450 kb on + strand, within H281DRAFT_02100 at 227.451 kb on - strand, within H281DRAFT_02100 at 227.451 kb on - strand, within H281DRAFT_02100 at 227.483 kb on + strand, within H281DRAFT_02100 at 227.484 kb on - strand, within H281DRAFT_02100 at 227.616 kb on + strand, within H281DRAFT_02100 at 227.616 kb on + strand, within H281DRAFT_02100 at 227.617 kb on - strand, within H281DRAFT_02100 at 227.617 kb on - strand, within H281DRAFT_02100 at 227.771 kb on + strand, within H281DRAFT_02100 at 227.772 kb on - strand, within H281DRAFT_02100 at 227.949 kb on + strand at 227.949 kb on + strand at 227.950 kb on - strand at 227.956 kb on + strand at 227.956 kb on + strand at 227.957 kb on - strand at 227.957 kb on - strand at 227.957 kb on - strand at 228.200 kb on - strand at 228.419 kb on - strand, within H281DRAFT_02101 at 228.419 kb on - strand, within H281DRAFT_02101 at 228.504 kb on + strand, within H281DRAFT_02101 at 228.604 kb on + strand, within H281DRAFT_02101 at 228.628 kb on + strand, within H281DRAFT_02101 at 228.659 kb on - strand, within H281DRAFT_02101 at 228.695 kb on - strand, within H281DRAFT_02101 at 228.739 kb on + strand, within H281DRAFT_02101 at 228.739 kb on + strand, within H281DRAFT_02101
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 225,994 + +0.6 225,994 + +4.8 226,421 + H281DRAFT_02099 0.63 +0.3 226,422 - H281DRAFT_02099 0.63 +0.3 226,824 - -0.7 226,829 + -0.0 226,829 + +0.1 226,969 - -4.2 227,083 + H281DRAFT_02100 0.14 +1.1 227,084 - H281DRAFT_02100 0.14 +0.3 227,260 - H281DRAFT_02100 0.33 +0.2 227,264 - H281DRAFT_02100 0.33 -0.8 227,264 - H281DRAFT_02100 0.33 +0.7 227,264 - H281DRAFT_02100 0.33 +0.1 227,283 + H281DRAFT_02100 0.35 +0.6 227,284 - H281DRAFT_02100 0.35 -0.9 227,304 + H281DRAFT_02100 0.37 +1.2 227,339 - H281DRAFT_02100 0.41 -3.8 227,339 - H281DRAFT_02100 0.41 +0.7 227,449 - H281DRAFT_02100 0.53 -0.4 227,450 + H281DRAFT_02100 0.53 +0.1 227,451 - H281DRAFT_02100 0.53 +0.3 227,451 - H281DRAFT_02100 0.53 -1.5 227,483 + H281DRAFT_02100 0.56 -0.3 227,484 - H281DRAFT_02100 0.56 -0.8 227,616 + H281DRAFT_02100 0.70 +0.8 227,616 + H281DRAFT_02100 0.70 +0.3 227,617 - H281DRAFT_02100 0.70 +0.2 227,617 - H281DRAFT_02100 0.70 +0.1 227,771 + H281DRAFT_02100 0.87 +0.1 227,772 - H281DRAFT_02100 0.87 +0.4 227,949 + -0.5 227,949 + -1.1 227,950 - +0.6 227,956 + +1.4 227,956 + -0.5 227,957 - -1.3 227,957 - -1.4 227,957 - -2.4 228,200 - +2.4 228,419 - H281DRAFT_02101 0.48 +0.6 228,419 - H281DRAFT_02101 0.48 +2.3 228,504 + H281DRAFT_02101 0.57 +0.3 228,604 + H281DRAFT_02101 0.68 +1.4 228,628 + H281DRAFT_02101 0.71 -0.8 228,659 - H281DRAFT_02101 0.74 -0.2 228,695 - H281DRAFT_02101 0.78 -0.9 228,739 + H281DRAFT_02101 0.83 -0.3 228,739 + H281DRAFT_02101 0.83 -0.4
Or see this region's nucleotide sequence