Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02097 and H281DRAFT_02098 are separated by 86 nucleotides H281DRAFT_02098 and H281DRAFT_02099 are separated by 191 nucleotides
H281DRAFT_02097: H281DRAFT_02097 - biotin synthase, at 223,610 to 224,737
_02097
H281DRAFT_02098: H281DRAFT_02098 - sulfate transport system substrate-binding protein, at 224,824 to 225,849
_02098
H281DRAFT_02099: H281DRAFT_02099 - hypothetical protein, at 226,041 to 226,643
_02099
Position (kb) 224
225
226 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 223.915 kb on + strand, within H281DRAFT_02097 at 224.160 kb on - strand, within H281DRAFT_02097 at 224.162 kb on + strand, within H281DRAFT_02097 at 224.162 kb on + strand, within H281DRAFT_02097 at 224.163 kb on - strand, within H281DRAFT_02097 at 224.223 kb on - strand at 224.223 kb on - strand, within H281DRAFT_02097 at 224.736 kb on - strand at 224.736 kb on - strand at 224.736 kb on - strand at 224.736 kb on - strand at 224.740 kb on - strand at 224.758 kb on - strand at 224.790 kb on - strand at 224.821 kb on + strand at 224.825 kb on + strand at 224.825 kb on + strand at 224.826 kb on - strand at 225.025 kb on + strand, within H281DRAFT_02098 at 225.025 kb on + strand, within H281DRAFT_02098 at 225.025 kb on + strand, within H281DRAFT_02098 at 225.026 kb on - strand, within H281DRAFT_02098 at 225.029 kb on + strand, within H281DRAFT_02098 at 225.030 kb on - strand, within H281DRAFT_02098 at 225.038 kb on + strand, within H281DRAFT_02098 at 225.350 kb on + strand, within H281DRAFT_02098 at 225.351 kb on - strand, within H281DRAFT_02098 at 225.351 kb on - strand, within H281DRAFT_02098 at 225.351 kb on - strand, within H281DRAFT_02098 at 225.354 kb on + strand, within H281DRAFT_02098 at 225.354 kb on + strand, within H281DRAFT_02098 at 225.354 kb on + strand, within H281DRAFT_02098 at 225.354 kb on + strand, within H281DRAFT_02098 at 225.355 kb on - strand, within H281DRAFT_02098 at 225.355 kb on - strand, within H281DRAFT_02098 at 225.355 kb on - strand, within H281DRAFT_02098 at 225.356 kb on + strand, within H281DRAFT_02098 at 225.475 kb on - strand, within H281DRAFT_02098 at 225.713 kb on + strand, within H281DRAFT_02098 at 225.735 kb on - strand, within H281DRAFT_02098 at 225.843 kb on - strand at 225.843 kb on - strand at 225.843 kb on - strand at 225.994 kb on + strand at 225.994 kb on + strand at 226.421 kb on + strand, within H281DRAFT_02099 at 226.422 kb on - strand, within H281DRAFT_02099 at 226.824 kb on - strand at 226.829 kb on + strand at 226.829 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 223,915 + H281DRAFT_02097 0.27 -3.7 224,160 - H281DRAFT_02097 0.49 -0.7 224,162 + H281DRAFT_02097 0.49 +0.3 224,162 + H281DRAFT_02097 0.49 +1.1 224,163 - H281DRAFT_02097 0.49 -1.0 224,223 - +3.1 224,223 - H281DRAFT_02097 0.54 +1.5 224,736 - +1.1 224,736 - -0.2 224,736 - -0.0 224,736 - +1.1 224,740 - -0.9 224,758 - -0.3 224,790 - +3.5 224,821 + -0.5 224,825 + -0.2 224,825 + +1.1 224,826 - +0.5 225,025 + H281DRAFT_02098 0.20 -0.1 225,025 + H281DRAFT_02098 0.20 +1.5 225,025 + H281DRAFT_02098 0.20 -0.1 225,026 - H281DRAFT_02098 0.20 -0.1 225,029 + H281DRAFT_02098 0.20 -0.5 225,030 - H281DRAFT_02098 0.20 +1.2 225,038 + H281DRAFT_02098 0.21 -3.5 225,350 + H281DRAFT_02098 0.51 -1.2 225,351 - H281DRAFT_02098 0.51 -0.2 225,351 - H281DRAFT_02098 0.51 +0.1 225,351 - H281DRAFT_02098 0.51 -0.0 225,354 + H281DRAFT_02098 0.52 -0.7 225,354 + H281DRAFT_02098 0.52 -0.4 225,354 + H281DRAFT_02098 0.52 -0.3 225,354 + H281DRAFT_02098 0.52 -1.7 225,355 - H281DRAFT_02098 0.52 -1.6 225,355 - H281DRAFT_02098 0.52 -1.4 225,355 - H281DRAFT_02098 0.52 -0.2 225,356 + H281DRAFT_02098 0.52 -1.0 225,475 - H281DRAFT_02098 0.63 -1.1 225,713 + H281DRAFT_02098 0.87 -0.2 225,735 - H281DRAFT_02098 0.89 -0.2 225,843 - -0.9 225,843 - +0.1 225,843 - +0.5 225,994 + +0.6 225,994 + +4.8 226,421 + H281DRAFT_02099 0.63 +0.3 226,422 - H281DRAFT_02099 0.63 +0.3 226,824 - -0.7 226,829 + -0.0 226,829 + +0.1
Or see this region's nucleotide sequence