Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02011 and H281DRAFT_02012 are separated by 247 nucleotides H281DRAFT_02012 and H281DRAFT_02013 are separated by 45 nucleotides H281DRAFT_02013 and H281DRAFT_02014 are separated by 466 nucleotides
H281DRAFT_02011: H281DRAFT_02011 - glutamate--cysteine ligase, at 128,218 to 129,507
_02011
H281DRAFT_02012: H281DRAFT_02012 - ammonium transporter, at 129,755 to 131,248
_02012
H281DRAFT_02013: H281DRAFT_02013 - nitrogen regulatory protein P-II family, at 131,294 to 131,632
_02013
H281DRAFT_02014: H281DRAFT_02014 - hypothetical protein, at 132,099 to 132,353
_02014
Position (kb) 129
130
131
132 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 128.805 kb on - strand at 128.868 kb on - strand, within H281DRAFT_02011 at 128.930 kb on + strand, within H281DRAFT_02011 at 128.931 kb on - strand, within H281DRAFT_02011 at 128.931 kb on - strand, within H281DRAFT_02011 at 129.380 kb on + strand at 129.380 kb on + strand at 129.380 kb on + strand at 129.380 kb on + strand at 129.381 kb on - strand at 129.381 kb on - strand at 129.482 kb on - strand at 129.489 kb on + strand at 129.493 kb on + strand at 129.493 kb on + strand at 129.493 kb on + strand at 129.494 kb on - strand at 129.494 kb on - strand at 129.539 kb on + strand at 129.539 kb on + strand at 129.539 kb on + strand at 129.618 kb on + strand at 129.646 kb on + strand at 129.757 kb on - strand at 129.876 kb on + strand at 129.876 kb on + strand at 129.876 kb on + strand at 129.876 kb on + strand at 129.876 kb on + strand at 129.876 kb on + strand at 130.183 kb on + strand, within H281DRAFT_02012 at 130.238 kb on + strand, within H281DRAFT_02012 at 130.238 kb on + strand, within H281DRAFT_02012 at 130.239 kb on - strand, within H281DRAFT_02012 at 130.530 kb on + strand, within H281DRAFT_02012 at 130.582 kb on + strand, within H281DRAFT_02012 at 130.582 kb on + strand, within H281DRAFT_02012 at 130.582 kb on + strand, within H281DRAFT_02012 at 130.583 kb on - strand, within H281DRAFT_02012 at 130.583 kb on - strand, within H281DRAFT_02012 at 130.583 kb on - strand, within H281DRAFT_02012 at 130.641 kb on + strand, within H281DRAFT_02012 at 130.642 kb on - strand, within H281DRAFT_02012 at 130.650 kb on + strand, within H281DRAFT_02012 at 130.650 kb on + strand, within H281DRAFT_02012 at 130.651 kb on - strand, within H281DRAFT_02012 at 130.719 kb on + strand, within H281DRAFT_02012 at 130.719 kb on + strand, within H281DRAFT_02012 at 130.719 kb on + strand, within H281DRAFT_02012 at 130.719 kb on + strand, within H281DRAFT_02012 at 130.719 kb on + strand, within H281DRAFT_02012 at 130.719 kb on + strand, within H281DRAFT_02012 at 130.719 kb on + strand, within H281DRAFT_02012 at 130.720 kb on - strand, within H281DRAFT_02012 at 130.720 kb on - strand, within H281DRAFT_02012 at 130.720 kb on - strand, within H281DRAFT_02012 at 130.720 kb on - strand, within H281DRAFT_02012 at 130.791 kb on + strand, within H281DRAFT_02012 at 130.792 kb on - strand, within H281DRAFT_02012 at 130.792 kb on - strand, within H281DRAFT_02012 at 130.859 kb on + strand, within H281DRAFT_02012 at 130.859 kb on + strand, within H281DRAFT_02012 at 130.859 kb on + strand, within H281DRAFT_02012 at 130.860 kb on - strand, within H281DRAFT_02012 at 130.888 kb on - strand, within H281DRAFT_02012 at 131.141 kb on - strand at 131.237 kb on + strand at 131.237 kb on + strand at 131.237 kb on + strand at 131.238 kb on - strand at 131.238 kb on - strand at 131.270 kb on + strand at 131.271 kb on - strand at 131.271 kb on - strand at 131.271 kb on - strand at 131.282 kb on + strand at 131.295 kb on + strand at 131.295 kb on + strand at 131.296 kb on - strand at 131.296 kb on - strand at 131.296 kb on - strand at 131.481 kb on + strand, within H281DRAFT_02013 at 131.481 kb on + strand, within H281DRAFT_02013 at 131.481 kb on + strand, within H281DRAFT_02013 at 131.496 kb on + strand, within H281DRAFT_02013 at 131.506 kb on + strand, within H281DRAFT_02013 at 131.506 kb on + strand, within H281DRAFT_02013 at 131.506 kb on + strand, within H281DRAFT_02013 at 131.646 kb on + strand at 131.646 kb on + strand at 131.646 kb on + strand at 131.646 kb on + strand at 131.647 kb on - strand at 131.647 kb on - strand at 131.647 kb on - strand at 131.647 kb on - strand at 131.647 kb on - strand at 131.647 kb on - strand at 131.679 kb on + strand at 131.692 kb on + strand at 131.732 kb on - strand at 131.756 kb on + strand at 131.757 kb on - strand at 131.819 kb on - strand at 131.901 kb on + strand at 131.943 kb on + strand at 131.943 kb on + strand at 131.943 kb on + strand at 131.943 kb on + strand at 131.943 kb on + strand at 131.944 kb on - strand at 131.944 kb on - strand at 131.944 kb on - strand at 132.057 kb on + strand at 132.057 kb on + strand at 132.058 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 128,805 - +1.1 128,868 - H281DRAFT_02011 0.50 -1.8 128,930 + H281DRAFT_02011 0.55 +3.0 128,931 - H281DRAFT_02011 0.55 +0.3 128,931 - H281DRAFT_02011 0.55 -0.1 129,380 + +1.7 129,380 + +1.7 129,380 + +3.2 129,380 + +1.2 129,381 - +0.3 129,381 - +0.6 129,482 - +0.6 129,489 + +2.3 129,493 + +2.1 129,493 + +2.3 129,493 + +2.1 129,494 - +0.5 129,494 - +0.8 129,539 + -0.3 129,539 + -0.0 129,539 + +1.3 129,618 + -3.3 129,646 + +0.9 129,757 - -1.8 129,876 + +0.4 129,876 + -0.5 129,876 + -0.1 129,876 + +0.2 129,876 + +0.5 129,876 + +0.1 130,183 + H281DRAFT_02012 0.29 +0.8 130,238 + H281DRAFT_02012 0.32 -1.6 130,238 + H281DRAFT_02012 0.32 -0.1 130,239 - H281DRAFT_02012 0.32 -0.7 130,530 + H281DRAFT_02012 0.52 +0.8 130,582 + H281DRAFT_02012 0.55 +1.4 130,582 + H281DRAFT_02012 0.55 +1.1 130,582 + H281DRAFT_02012 0.55 +0.5 130,583 - H281DRAFT_02012 0.55 +0.1 130,583 - H281DRAFT_02012 0.55 +0.8 130,583 - H281DRAFT_02012 0.55 +0.8 130,641 + H281DRAFT_02012 0.59 +0.1 130,642 - H281DRAFT_02012 0.59 +0.1 130,650 + H281DRAFT_02012 0.60 -1.1 130,650 + H281DRAFT_02012 0.60 +0.2 130,651 - H281DRAFT_02012 0.60 -1.1 130,719 + H281DRAFT_02012 0.65 +0.4 130,719 + H281DRAFT_02012 0.65 +1.1 130,719 + H281DRAFT_02012 0.65 +2.4 130,719 + H281DRAFT_02012 0.65 -0.9 130,719 + H281DRAFT_02012 0.65 +0.2 130,719 + H281DRAFT_02012 0.65 -0.9 130,719 + H281DRAFT_02012 0.65 -0.2 130,720 - H281DRAFT_02012 0.65 -0.6 130,720 - H281DRAFT_02012 0.65 -0.0 130,720 - H281DRAFT_02012 0.65 +0.2 130,720 - H281DRAFT_02012 0.65 -0.1 130,791 + H281DRAFT_02012 0.69 +2.1 130,792 - H281DRAFT_02012 0.69 -0.7 130,792 - H281DRAFT_02012 0.69 -1.6 130,859 + H281DRAFT_02012 0.74 -3.1 130,859 + H281DRAFT_02012 0.74 -0.7 130,859 + H281DRAFT_02012 0.74 +0.7 130,860 - H281DRAFT_02012 0.74 +0.1 130,888 - H281DRAFT_02012 0.76 +1.0 131,141 - -1.1 131,237 + -0.2 131,237 + +0.9 131,237 + +0.6 131,238 - +1.2 131,238 - -0.8 131,270 + -1.8 131,271 - +0.9 131,271 - -0.9 131,271 - +0.7 131,282 + -0.3 131,295 + -0.1 131,295 + -2.6 131,296 - -0.3 131,296 - +0.3 131,296 - -0.7 131,481 + H281DRAFT_02013 0.55 +0.6 131,481 + H281DRAFT_02013 0.55 +0.4 131,481 + H281DRAFT_02013 0.55 +0.0 131,496 + H281DRAFT_02013 0.60 -1.0 131,506 + H281DRAFT_02013 0.63 +1.1 131,506 + H281DRAFT_02013 0.63 -0.4 131,506 + H281DRAFT_02013 0.63 -0.2 131,646 + +0.4 131,646 + -1.9 131,646 + -2.1 131,646 + -0.0 131,647 - -0.3 131,647 - +0.5 131,647 - -0.4 131,647 - -0.8 131,647 - +0.7 131,647 - +0.9 131,679 + +1.8 131,692 + +1.3 131,732 - -0.4 131,756 + +0.1 131,757 - +0.5 131,819 - -0.3 131,901 + -0.2 131,943 + -0.3 131,943 + -1.1 131,943 + -1.9 131,943 + +0.8 131,943 + -0.4 131,944 - -0.2 131,944 - +0.4 131,944 - -2.5 132,057 + -0.0 132,057 + -0.1 132,058 - -0.3
Or see this region's nucleotide sequence