Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01683 and H281DRAFT_01684 are separated by 194 nucleotides H281DRAFT_01684 and H281DRAFT_01685 are separated by 184 nucleotides
H281DRAFT_01683: H281DRAFT_01683 - ketopantoate reductase (EC 1.1.1.169), at 565,870 to 566,820
_01683
H281DRAFT_01684: H281DRAFT_01684 - transcriptional regulator, TetR family, at 567,015 to 567,590
_01684
H281DRAFT_01685: H281DRAFT_01685 - aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit A, at 567,775 to 569,184
_01685
Position (kb)
567
568 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 566.198 kb on + strand, within H281DRAFT_01683 at 566.210 kb on + strand, within H281DRAFT_01683 at 566.210 kb on + strand, within H281DRAFT_01683 at 566.211 kb on - strand, within H281DRAFT_01683 at 566.444 kb on + strand, within H281DRAFT_01683 at 566.444 kb on + strand, within H281DRAFT_01683 at 567.043 kb on + strand at 567.044 kb on - strand at 567.121 kb on + strand, within H281DRAFT_01684 at 567.121 kb on + strand, within H281DRAFT_01684 at 567.122 kb on - strand, within H281DRAFT_01684 at 567.256 kb on + strand, within H281DRAFT_01684 at 567.454 kb on + strand, within H281DRAFT_01684 at 567.454 kb on + strand, within H281DRAFT_01684 at 567.455 kb on - strand, within H281DRAFT_01684 at 567.455 kb on - strand, within H281DRAFT_01684 at 567.500 kb on + strand, within H281DRAFT_01684 at 567.774 kb on + strand at 567.929 kb on + strand, within H281DRAFT_01685 at 567.929 kb on + strand, within H281DRAFT_01685 at 568.059 kb on + strand, within H281DRAFT_01685 at 568.204 kb on - strand, within H281DRAFT_01685 at 568.272 kb on + strand, within H281DRAFT_01685 at 568.273 kb on - strand, within H281DRAFT_01685 at 568.273 kb on - strand, within H281DRAFT_01685 at 568.370 kb on + strand, within H281DRAFT_01685 at 568.435 kb on - strand, within H281DRAFT_01685
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 566,198 + H281DRAFT_01683 0.34 +0.7 566,210 + H281DRAFT_01683 0.36 -1.9 566,210 + H281DRAFT_01683 0.36 +0.3 566,211 - H281DRAFT_01683 0.36 +0.4 566,444 + H281DRAFT_01683 0.60 -0.2 566,444 + H281DRAFT_01683 0.60 +0.3 567,043 + -1.5 567,044 - -0.8 567,121 + H281DRAFT_01684 0.18 +1.6 567,121 + H281DRAFT_01684 0.18 -1.0 567,122 - H281DRAFT_01684 0.19 -0.9 567,256 + H281DRAFT_01684 0.42 -0.5 567,454 + H281DRAFT_01684 0.76 -1.0 567,454 + H281DRAFT_01684 0.76 -3.8 567,455 - H281DRAFT_01684 0.76 +1.7 567,455 - H281DRAFT_01684 0.76 -2.5 567,500 + H281DRAFT_01684 0.84 -1.3 567,774 + +0.1 567,929 + H281DRAFT_01685 0.11 -0.9 567,929 + H281DRAFT_01685 0.11 +0.7 568,059 + H281DRAFT_01685 0.20 -0.3 568,204 - H281DRAFT_01685 0.30 +0.8 568,272 + H281DRAFT_01685 0.35 -3.1 568,273 - H281DRAFT_01685 0.35 -0.9 568,273 - H281DRAFT_01685 0.35 -0.6 568,370 + H281DRAFT_01685 0.42 -0.7 568,435 - H281DRAFT_01685 0.47 +0.5
Or see this region's nucleotide sequence