Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00531 and H281DRAFT_00532 are separated by 177 nucleotides H281DRAFT_00532 and H281DRAFT_00533 are separated by 64 nucleotides H281DRAFT_00533 and H281DRAFT_00534 are separated by 695 nucleotides
H281DRAFT_00531: H281DRAFT_00531 - hypothetical protein, at 596,428 to 597,186
_00531
H281DRAFT_00532: H281DRAFT_00532 - hypothetical protein, at 597,364 to 597,582
_00532
H281DRAFT_00533: H281DRAFT_00533 - ATP-dependent DNA helicase DinG, at 597,647 to 599,911
_00533
H281DRAFT_00534: H281DRAFT_00534 - 50S ribosomal protein L16 3-hydroxylase, at 600,607 to 601,857
_00534
Position (kb) 597
598
599
600 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 596.783 kb on - strand, within H281DRAFT_00531 at 596.866 kb on + strand, within H281DRAFT_00531 at 596.951 kb on - strand, within H281DRAFT_00531 at 597.190 kb on + strand at 597.206 kb on + strand at 597.206 kb on + strand at 597.206 kb on + strand at 597.238 kb on + strand at 597.238 kb on + strand at 597.238 kb on + strand at 597.239 kb on - strand at 597.241 kb on + strand at 597.241 kb on + strand at 597.250 kb on - strand at 597.270 kb on + strand at 597.276 kb on + strand at 597.572 kb on + strand at 597.572 kb on + strand at 597.573 kb on - strand at 598.064 kb on + strand, within H281DRAFT_00533 at 598.064 kb on + strand, within H281DRAFT_00533 at 598.065 kb on - strand, within H281DRAFT_00533 at 598.071 kb on - strand, within H281DRAFT_00533 at 598.156 kb on + strand, within H281DRAFT_00533 at 598.224 kb on - strand, within H281DRAFT_00533 at 598.224 kb on - strand, within H281DRAFT_00533 at 598.224 kb on - strand, within H281DRAFT_00533 at 598.224 kb on - strand, within H281DRAFT_00533 at 598.224 kb on - strand, within H281DRAFT_00533 at 598.224 kb on - strand, within H281DRAFT_00533 at 598.235 kb on + strand, within H281DRAFT_00533 at 598.238 kb on + strand, within H281DRAFT_00533 at 598.238 kb on + strand, within H281DRAFT_00533 at 598.238 kb on + strand, within H281DRAFT_00533 at 598.239 kb on - strand, within H281DRAFT_00533 at 598.239 kb on - strand, within H281DRAFT_00533 at 598.405 kb on + strand, within H281DRAFT_00533 at 598.405 kb on + strand, within H281DRAFT_00533 at 598.405 kb on + strand, within H281DRAFT_00533 at 598.406 kb on - strand, within H281DRAFT_00533 at 598.434 kb on - strand, within H281DRAFT_00533 at 598.834 kb on - strand, within H281DRAFT_00533 at 599.201 kb on + strand, within H281DRAFT_00533 at 599.236 kb on + strand, within H281DRAFT_00533 at 599.237 kb on - strand, within H281DRAFT_00533 at 599.282 kb on + strand, within H281DRAFT_00533 at 599.282 kb on + strand, within H281DRAFT_00533 at 599.283 kb on - strand, within H281DRAFT_00533 at 599.392 kb on + strand, within H281DRAFT_00533 at 599.392 kb on + strand, within H281DRAFT_00533 at 599.392 kb on + strand, within H281DRAFT_00533 at 599.462 kb on + strand, within H281DRAFT_00533 at 599.720 kb on - strand at 599.804 kb on - strand at 599.804 kb on - strand at 599.974 kb on + strand at 599.982 kb on + strand at 600.512 kb on - strand at 600.552 kb on - strand at 600.583 kb on - strand at 600.583 kb on - strand at 600.583 kb on - strand at 600.583 kb on - strand at 600.589 kb on + strand at 600.590 kb on - strand at 600.590 kb on - strand at 600.590 kb on - strand at 600.592 kb on - strand at 600.644 kb on + strand at 600.645 kb on - strand at 600.656 kb on - strand at 600.656 kb on - strand at 600.692 kb on - strand at 600.711 kb on + strand at 600.807 kb on + strand at 600.808 kb on - strand, within H281DRAFT_00534 at 600.853 kb on - strand, within H281DRAFT_00534
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 596,783 - H281DRAFT_00531 0.47 +0.8 596,866 + H281DRAFT_00531 0.58 -1.6 596,951 - H281DRAFT_00531 0.69 -2.1 597,190 + -1.7 597,206 + +1.0 597,206 + -0.4 597,206 + +0.0 597,238 + -0.8 597,238 + -0.4 597,238 + +0.4 597,239 - +0.3 597,241 + -0.3 597,241 + -2.8 597,250 - +0.7 597,270 + +0.9 597,276 + +0.1 597,572 + -0.2 597,572 + -0.1 597,573 - -0.5 598,064 + H281DRAFT_00533 0.18 -0.5 598,064 + H281DRAFT_00533 0.18 +0.3 598,065 - H281DRAFT_00533 0.18 +0.2 598,071 - H281DRAFT_00533 0.19 +1.1 598,156 + H281DRAFT_00533 0.22 -0.6 598,224 - H281DRAFT_00533 0.25 +1.1 598,224 - H281DRAFT_00533 0.25 -0.7 598,224 - H281DRAFT_00533 0.25 -0.2 598,224 - H281DRAFT_00533 0.25 +0.8 598,224 - H281DRAFT_00533 0.25 +0.5 598,224 - H281DRAFT_00533 0.25 -2.0 598,235 + H281DRAFT_00533 0.26 +0.2 598,238 + H281DRAFT_00533 0.26 +0.5 598,238 + H281DRAFT_00533 0.26 -0.5 598,238 + H281DRAFT_00533 0.26 +2.8 598,239 - H281DRAFT_00533 0.26 -0.2 598,239 - H281DRAFT_00533 0.26 -0.5 598,405 + H281DRAFT_00533 0.33 +0.8 598,405 + H281DRAFT_00533 0.33 -0.1 598,405 + H281DRAFT_00533 0.33 +0.9 598,406 - H281DRAFT_00533 0.34 -0.0 598,434 - H281DRAFT_00533 0.35 -0.8 598,834 - H281DRAFT_00533 0.52 -2.5 599,201 + H281DRAFT_00533 0.69 +0.0 599,236 + H281DRAFT_00533 0.70 -0.6 599,237 - H281DRAFT_00533 0.70 -0.6 599,282 + H281DRAFT_00533 0.72 -0.6 599,282 + H281DRAFT_00533 0.72 +0.6 599,283 - H281DRAFT_00533 0.72 +0.4 599,392 + H281DRAFT_00533 0.77 -0.2 599,392 + H281DRAFT_00533 0.77 +0.3 599,392 + H281DRAFT_00533 0.77 +0.5 599,462 + H281DRAFT_00533 0.80 +0.2 599,720 - +1.2 599,804 - +2.5 599,804 - -0.6 599,974 + -1.7 599,982 + -0.0 600,512 - +2.1 600,552 - +0.2 600,583 - +0.4 600,583 - -0.9 600,583 - +0.5 600,583 - -1.9 600,589 + +0.4 600,590 - -1.2 600,590 - +0.6 600,590 - -1.3 600,592 - -0.2 600,644 + +0.1 600,645 - -0.3 600,656 - -0.1 600,656 - +2.7 600,692 - +0.6 600,711 + -0.2 600,807 + +2.4 600,808 - H281DRAFT_00534 0.16 -0.1 600,853 - H281DRAFT_00534 0.20 -1.0
Or see this region's nucleotide sequence