Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00504 and H281DRAFT_00505 are separated by 109 nucleotides H281DRAFT_00505 and H281DRAFT_00506 are separated by 47 nucleotides H281DRAFT_00506 and H281DRAFT_00507 are separated by 6 nucleotides
H281DRAFT_00504: H281DRAFT_00504 - hypothetical protein, at 565,512 to 566,345
_00504
H281DRAFT_00505: H281DRAFT_00505 - tRNA threonylcarbamoyl adenosine modification protein, Sua5/YciO/YrdC/YwlC family, at 566,455 to 567,090
_00505
H281DRAFT_00506: H281DRAFT_00506 - Zn-dependent protease (includes SpoIVFB), at 567,138 to 567,800
_00506
H281DRAFT_00507: H281DRAFT_00507 - tryptophanyl-tRNA synthetase, at 567,807 to 569,009
_00507
Position (kb) 567
568 Strain fitness (log2 ratio) -1
0
1 at 566.419 kb on + strand at 566.743 kb on + strand, within H281DRAFT_00505 at 566.743 kb on + strand, within H281DRAFT_00505 at 566.743 kb on + strand, within H281DRAFT_00505 at 566.744 kb on - strand, within H281DRAFT_00505 at 566.891 kb on + strand, within H281DRAFT_00505 at 567.124 kb on + strand at 567.125 kb on - strand at 567.125 kb on - strand at 567.137 kb on + strand at 567.154 kb on + strand at 567.255 kb on + strand, within H281DRAFT_00506 at 567.366 kb on + strand, within H281DRAFT_00506 at 567.755 kb on + strand at 567.755 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 566,419 + +0.5 566,743 + H281DRAFT_00505 0.45 -1.0 566,743 + H281DRAFT_00505 0.45 -0.6 566,743 + H281DRAFT_00505 0.45 +0.6 566,744 - H281DRAFT_00505 0.45 +0.1 566,891 + H281DRAFT_00505 0.69 -0.9 567,124 + +0.9 567,125 - +1.4 567,125 - +1.3 567,137 + -0.7 567,154 + +0.8 567,255 + H281DRAFT_00506 0.18 -0.0 567,366 + H281DRAFT_00506 0.34 -0.9 567,755 + -0.2 567,755 + -0.1
Or see this region's nucleotide sequence