Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00383 and H281DRAFT_00384 overlap by 1 nucleotides H281DRAFT_00384 and H281DRAFT_00385 are separated by 125 nucleotides H281DRAFT_00385 and H281DRAFT_00386 are separated by 302 nucleotides H281DRAFT_00386 and H281DRAFT_00387 are separated by 61 nucleotides
H281DRAFT_00383: H281DRAFT_00383 - amino acid ABC transporter membrane protein, PAAT family, at 423,590 to 424,267
_00383
H281DRAFT_00384: H281DRAFT_00384 - amino acid ABC transporter ATP-binding protein, PAAT family, at 424,267 to 425,034
_00384
H281DRAFT_00385: H281DRAFT_00385 - 2-isopropylmalate synthase, at 425,160 to 426,878
_00385
H281DRAFT_00386: H281DRAFT_00386 - hypothetical protein, at 427,181 to 427,687
_00386
H281DRAFT_00387: H281DRAFT_00387 - hypothetical protein, at 427,749 to 429,617
_00387
Position (kb) 425
426
427 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 424.179 kb on - strand, within H281DRAFT_00383 at 424.389 kb on + strand, within H281DRAFT_00384 at 424.754 kb on + strand, within H281DRAFT_00384 at 424.755 kb on - strand, within H281DRAFT_00384 at 424.755 kb on - strand, within H281DRAFT_00384 at 424.755 kb on - strand, within H281DRAFT_00384 at 424.761 kb on - strand, within H281DRAFT_00384 at 424.817 kb on + strand, within H281DRAFT_00384 at 424.912 kb on - strand, within H281DRAFT_00384 at 424.923 kb on - strand, within H281DRAFT_00384 at 425.060 kb on + strand at 425.128 kb on + strand at 425.129 kb on - strand at 425.150 kb on + strand at 425.162 kb on - strand at 425.398 kb on + strand, within H281DRAFT_00385 at 425.717 kb on - strand, within H281DRAFT_00385 at 425.768 kb on - strand, within H281DRAFT_00385 at 425.803 kb on + strand, within H281DRAFT_00385 at 425.804 kb on - strand, within H281DRAFT_00385 at 425.906 kb on - strand, within H281DRAFT_00385 at 426.214 kb on + strand, within H281DRAFT_00385 at 426.496 kb on + strand, within H281DRAFT_00385 at 426.497 kb on - strand, within H281DRAFT_00385 at 426.497 kb on - strand, within H281DRAFT_00385 at 426.853 kb on + strand at 426.861 kb on - strand at 426.909 kb on - strand at 426.971 kb on + strand at 427.050 kb on + strand at 427.050 kb on + strand at 427.051 kb on - strand at 427.051 kb on - strand at 427.100 kb on + strand at 427.101 kb on - strand at 427.316 kb on - strand, within H281DRAFT_00386 at 427.621 kb on + strand, within H281DRAFT_00386 at 427.621 kb on + strand at 427.750 kb on + strand at 427.750 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 424,179 - H281DRAFT_00383 0.87 +0.3 424,389 + H281DRAFT_00384 0.16 -1.8 424,754 + H281DRAFT_00384 0.63 +0.6 424,755 - H281DRAFT_00384 0.64 -3.4 424,755 - H281DRAFT_00384 0.64 +0.9 424,755 - H281DRAFT_00384 0.64 +0.7 424,761 - H281DRAFT_00384 0.64 -1.6 424,817 + H281DRAFT_00384 0.72 -0.2 424,912 - H281DRAFT_00384 0.84 -1.1 424,923 - H281DRAFT_00384 0.85 +0.8 425,060 + -0.9 425,128 + +0.6 425,129 - +0.1 425,150 + +0.6 425,162 - +3.7 425,398 + H281DRAFT_00385 0.14 -1.4 425,717 - H281DRAFT_00385 0.32 -0.7 425,768 - H281DRAFT_00385 0.35 -2.6 425,803 + H281DRAFT_00385 0.37 -2.1 425,804 - H281DRAFT_00385 0.37 +0.7 425,906 - H281DRAFT_00385 0.43 +0.3 426,214 + H281DRAFT_00385 0.61 +0.1 426,496 + H281DRAFT_00385 0.78 +0.7 426,497 - H281DRAFT_00385 0.78 +0.2 426,497 - H281DRAFT_00385 0.78 -0.4 426,853 + +0.6 426,861 - -1.0 426,909 - -0.2 426,971 + +2.9 427,050 + -0.5 427,050 + -0.2 427,051 - -0.3 427,051 - -0.1 427,100 + -0.3 427,101 - +0.2 427,316 - H281DRAFT_00386 0.27 -1.2 427,621 + H281DRAFT_00386 0.87 -0.1 427,621 + +0.9 427,750 + +2.9 427,750 + -0.5
Or see this region's nucleotide sequence