Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00382 and H281DRAFT_00383 are separated by 22 nucleotides H281DRAFT_00383 and H281DRAFT_00384 overlap by 1 nucleotides H281DRAFT_00384 and H281DRAFT_00385 are separated by 125 nucleotides
H281DRAFT_00382: H281DRAFT_00382 - amino acid ABC transporter substrate-binding protein, PAAT family, at 422,785 to 423,567
_00382
H281DRAFT_00383: H281DRAFT_00383 - amino acid ABC transporter membrane protein, PAAT family, at 423,590 to 424,267
_00383
H281DRAFT_00384: H281DRAFT_00384 - amino acid ABC transporter ATP-binding protein, PAAT family, at 424,267 to 425,034
_00384
H281DRAFT_00385: H281DRAFT_00385 - 2-isopropylmalate synthase, at 425,160 to 426,878
_00385
Position (kb) 423
424
425 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 422.593 kb on - strand at 422.613 kb on - strand at 422.914 kb on + strand, within H281DRAFT_00382 at 422.915 kb on - strand, within H281DRAFT_00382 at 422.915 kb on - strand, within H281DRAFT_00382 at 423.541 kb on + strand at 423.541 kb on + strand at 423.636 kb on + strand at 423.639 kb on - strand at 423.639 kb on - strand at 423.764 kb on + strand, within H281DRAFT_00383 at 423.897 kb on - strand, within H281DRAFT_00383 at 424.158 kb on - strand, within H281DRAFT_00383 at 424.179 kb on - strand, within H281DRAFT_00383 at 424.389 kb on + strand, within H281DRAFT_00384 at 424.754 kb on + strand, within H281DRAFT_00384 at 424.755 kb on - strand, within H281DRAFT_00384 at 424.755 kb on - strand, within H281DRAFT_00384 at 424.755 kb on - strand, within H281DRAFT_00384 at 424.761 kb on - strand, within H281DRAFT_00384 at 424.817 kb on + strand, within H281DRAFT_00384 at 424.912 kb on - strand, within H281DRAFT_00384 at 424.923 kb on - strand, within H281DRAFT_00384 at 425.060 kb on + strand at 425.128 kb on + strand at 425.129 kb on - strand at 425.150 kb on + strand at 425.162 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 422,593 - -0.6 422,613 - +0.8 422,914 + H281DRAFT_00382 0.16 +0.6 422,915 - H281DRAFT_00382 0.17 +0.7 422,915 - H281DRAFT_00382 0.17 +1.2 423,541 + -1.1 423,541 + -0.6 423,636 + +0.3 423,639 - +0.7 423,639 - -0.6 423,764 + H281DRAFT_00383 0.26 -0.3 423,897 - H281DRAFT_00383 0.45 +0.2 424,158 - H281DRAFT_00383 0.84 -0.7 424,179 - H281DRAFT_00383 0.87 +0.3 424,389 + H281DRAFT_00384 0.16 -1.8 424,754 + H281DRAFT_00384 0.63 +0.6 424,755 - H281DRAFT_00384 0.64 -3.4 424,755 - H281DRAFT_00384 0.64 +0.9 424,755 - H281DRAFT_00384 0.64 +0.7 424,761 - H281DRAFT_00384 0.64 -1.6 424,817 + H281DRAFT_00384 0.72 -0.2 424,912 - H281DRAFT_00384 0.84 -1.1 424,923 - H281DRAFT_00384 0.85 +0.8 425,060 + -0.9 425,128 + +0.6 425,129 - +0.1 425,150 + +0.6 425,162 - +3.7
Or see this region's nucleotide sequence