Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00331 and H281DRAFT_00332 are separated by 160 nucleotides H281DRAFT_00332 and H281DRAFT_00333 are separated by 138 nucleotides
H281DRAFT_00331: H281DRAFT_00331 - EAL and modified HD-GYP domain-containing signal transduction protein, at 362,492 to 363,766
_00331
H281DRAFT_00332: H281DRAFT_00332 - methyl-accepting chemotaxis protein, at 363,927 to 365,624
_00332
H281DRAFT_00333: H281DRAFT_00333 - L-ascorbate metabolism protein UlaG, beta-lactamase superfamily, at 365,763 to 366,542
_00333
Position (kb) 363
364
365
366 Strain fitness (log2 ratio) -2
-1
0
1 at 363.195 kb on + strand, within H281DRAFT_00331 at 363.934 kb on - strand at 363.946 kb on + strand at 364.234 kb on - strand, within H281DRAFT_00332 at 364.368 kb on - strand, within H281DRAFT_00332 at 364.470 kb on - strand, within H281DRAFT_00332 at 364.682 kb on - strand, within H281DRAFT_00332 at 365.280 kb on - strand, within H281DRAFT_00332 at 365.732 kb on + strand at 365.733 kb on - strand at 366.071 kb on - strand, within H281DRAFT_00333 at 366.352 kb on - strand, within H281DRAFT_00333 at 366.361 kb on - strand, within H281DRAFT_00333 at 366.440 kb on - strand, within H281DRAFT_00333 at 366.611 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 363,195 + H281DRAFT_00331 0.55 -0.4 363,934 - -1.8 363,946 + -1.4 364,234 - H281DRAFT_00332 0.18 +0.5 364,368 - H281DRAFT_00332 0.26 +1.2 364,470 - H281DRAFT_00332 0.32 +0.8 364,682 - H281DRAFT_00332 0.44 +0.2 365,280 - H281DRAFT_00332 0.80 +0.3 365,732 + -0.2 365,733 - -0.5 366,071 - H281DRAFT_00333 0.39 -0.8 366,352 - H281DRAFT_00333 0.76 +0.4 366,361 - H281DRAFT_00333 0.77 -1.4 366,440 - H281DRAFT_00333 0.87 -0.3 366,611 + -0.7
Or see this region's nucleotide sequence