Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_00010

Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_00008 and H281DRAFT_00009 overlap by 4 nucleotidesH281DRAFT_00009 and H281DRAFT_00010 are separated by 300 nucleotidesH281DRAFT_00010 and H281DRAFT_00011 overlap by 4 nucleotidesH281DRAFT_00011 and H281DRAFT_00012 are separated by 1 nucleotides H281DRAFT_00008: H281DRAFT_00008 - hypothetical protein, at 8,258 to 8,575 _00008 H281DRAFT_00009: H281DRAFT_00009 - RepB plasmid partitioning protein, at 8,572 to 9,186 _00009 H281DRAFT_00010: H281DRAFT_00010 - nitrile hydratase, at 9,487 to 10,146 _00010 H281DRAFT_00011: H281DRAFT_00011 - nitrile hydratase, at 10,143 to 10,814 _00011 H281DRAFT_00012: H281DRAFT_00012 - nitrile hydratase accessory protein, at 10,816 to 11,184 _00012 Position (kb) 9 10 11Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 8.799 kb on - strand, within H281DRAFT_00009at 8.799 kb on - strand, within H281DRAFT_00009at 8.938 kb on + strand, within H281DRAFT_00009at 8.954 kb on - strand, within H281DRAFT_00009at 8.954 kb on - strand, within H281DRAFT_00009at 8.957 kb on - strand, within H281DRAFT_00009at 8.957 kb on - strand, within H281DRAFT_00009at 9.020 kb on + strand, within H281DRAFT_00009at 9.115 kb on + strand, within H281DRAFT_00009at 9.220 kb on + strandat 9.221 kb on - strandat 9.238 kb on + strandat 9.268 kb on + strandat 9.269 kb on - strandat 9.310 kb on - strandat 9.450 kb on + strandat 9.450 kb on + strandat 9.608 kb on - strand, within H281DRAFT_00010at 9.647 kb on - strand, within H281DRAFT_00010at 9.686 kb on + strand, within H281DRAFT_00010at 9.713 kb on - strand, within H281DRAFT_00010at 9.834 kb on + strand, within H281DRAFT_00010at 9.935 kb on + strand, within H281DRAFT_00010at 10.142 kb on + strandat 10.142 kb on + strandat 10.142 kb on + strandat 10.143 kb on - strandat 10.143 kb on - strandat 10.143 kb on - strandat 10.143 kb on - strandat 10.143 kb on - strandat 10.143 kb on - strandat 10.143 kb on - strandat 10.330 kb on - strand, within H281DRAFT_00011at 10.345 kb on - strand, within H281DRAFT_00011at 10.347 kb on + strand, within H281DRAFT_00011at 10.348 kb on - strand, within H281DRAFT_00011at 10.444 kb on + strand, within H281DRAFT_00011at 10.444 kb on + strand, within H281DRAFT_00011at 10.602 kb on + strand, within H281DRAFT_00011at 10.704 kb on - strand, within H281DRAFT_00011at 10.704 kb on - strand, within H281DRAFT_00011at 10.782 kb on + strandat 10.982 kb on - strand, within H281DRAFT_00012at 11.041 kb on + strand, within H281DRAFT_00012at 11.041 kb on + strand, within H281DRAFT_00012at 11.042 kb on - strand, within H281DRAFT_00012at 11.042 kb on - strand, within H281DRAFT_00012at 11.045 kb on - strand, within H281DRAFT_00012

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
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8,799 - H281DRAFT_00009 0.37 -0.6
8,799 - H281DRAFT_00009 0.37 -2.8
8,938 + H281DRAFT_00009 0.60 +0.9
8,954 - H281DRAFT_00009 0.62 -0.3
8,954 - H281DRAFT_00009 0.62 -1.3
8,957 - H281DRAFT_00009 0.63 +0.1
8,957 - H281DRAFT_00009 0.63 +1.1
9,020 + H281DRAFT_00009 0.73 -1.1
9,115 + H281DRAFT_00009 0.88 -0.8
9,220 + -0.4
9,221 - -0.2
9,238 + -0.4
9,268 + -0.0
9,269 - +0.9
9,310 - -0.1
9,450 + -0.2
9,450 + -0.1
9,608 - H281DRAFT_00010 0.18 -2.0
9,647 - H281DRAFT_00010 0.24 -1.0
9,686 + H281DRAFT_00010 0.30 -0.3
9,713 - H281DRAFT_00010 0.34 +0.3
9,834 + H281DRAFT_00010 0.53 +0.2
9,935 + H281DRAFT_00010 0.68 +0.2
10,142 + +0.1
10,142 + +1.0
10,142 + -0.8
10,143 - -0.2
10,143 - +1.3
10,143 - +1.2
10,143 - +0.5
10,143 - -2.7
10,143 - -0.6
10,143 - +0.7
10,330 - H281DRAFT_00011 0.28 -0.6
10,345 - H281DRAFT_00011 0.30 +0.2
10,347 + H281DRAFT_00011 0.30 -2.0
10,348 - H281DRAFT_00011 0.31 -0.0
10,444 + H281DRAFT_00011 0.45 -3.8
10,444 + H281DRAFT_00011 0.45 -1.4
10,602 + H281DRAFT_00011 0.68 -0.7
10,704 - H281DRAFT_00011 0.83 +0.7
10,704 - H281DRAFT_00011 0.83 -0.6
10,782 + -0.3
10,982 - H281DRAFT_00012 0.45 -0.4
11,041 + H281DRAFT_00012 0.61 -2.8
11,041 + H281DRAFT_00012 0.61 -0.1
11,042 - H281DRAFT_00012 0.61 -0.2
11,042 - H281DRAFT_00012 0.61 +0.9
11,045 - H281DRAFT_00012 0.62 +1.5

Or see this region's nucleotide sequence