Experiment: Rifampicin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_16525 and AO353_16530 are separated by 17 nucleotides AO353_16530 and AO353_16535 are separated by 76 nucleotides AO353_16535 and AO353_16540 are separated by 153 nucleotides
AO353_16525: AO353_16525 - lipid-A-disaccharide synthase, at 3,668,657 to 3,669,790
_16525
AO353_16530: AO353_16530 - ribonuclease HII, at 3,669,808 to 3,670,428
_16530
AO353_16535: AO353_16535 - DNA polymerase III subunit alpha, at 3,670,505 to 3,674,026
_16535
AO353_16540: AO353_16540 - acetyl-CoA carboxylase subunit alpha, at 3,674,180 to 3,675,127
_16540
Position (kb)
3670
3671
3672
3673
3674
3675 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3669.797 kb on + strand at 3669.797 kb on + strand at 3669.797 kb on + strand at 3669.797 kb on + strand at 3669.798 kb on - strand at 3669.798 kb on - strand at 3669.798 kb on - strand at 3669.798 kb on - strand at 3669.798 kb on - strand at 3669.798 kb on - strand at 3669.823 kb on - strand at 3669.989 kb on - strand, within AO353_16530 at 3670.062 kb on + strand, within AO353_16530 at 3670.063 kb on - strand, within AO353_16530 at 3670.396 kb on + strand at 3670.396 kb on + strand at 3670.396 kb on + strand at 3670.396 kb on + strand at 3670.397 kb on - strand at 3670.397 kb on - strand at 3670.397 kb on - strand at 3670.397 kb on - strand at 3670.397 kb on - strand at 3670.426 kb on + strand at 3670.426 kb on + strand at 3670.426 kb on + strand at 3670.426 kb on + strand at 3670.426 kb on + strand at 3670.426 kb on + strand at 3670.426 kb on + strand at 3670.427 kb on - strand at 3670.427 kb on - strand at 3670.427 kb on - strand at 3670.427 kb on - strand at 3670.461 kb on - strand at 3674.045 kb on + strand at 3674.083 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.005 mg/ml remove 3,669,797 + -0.8 3,669,797 + -0.8 3,669,797 + +1.7 3,669,797 + +0.5 3,669,798 - -1.6 3,669,798 - +0.0 3,669,798 - +0.4 3,669,798 - -2.3 3,669,798 - -1.1 3,669,798 - -1.4 3,669,823 - +1.3 3,669,989 - AO353_16530 0.29 +0.4 3,670,062 + AO353_16530 0.41 -1.7 3,670,063 - AO353_16530 0.41 +0.5 3,670,396 + +0.4 3,670,396 + +0.5 3,670,396 + +0.1 3,670,396 + -1.0 3,670,397 - -0.3 3,670,397 - +0.1 3,670,397 - +0.2 3,670,397 - +2.0 3,670,397 - -0.4 3,670,426 + -1.1 3,670,426 + +0.4 3,670,426 + +0.0 3,670,426 + -0.7 3,670,426 + +0.0 3,670,426 + -0.1 3,670,426 + +0.4 3,670,427 - +0.0 3,670,427 - +1.6 3,670,427 - +0.1 3,670,427 - +2.3 3,670,461 - -0.3 3,674,045 + -0.6 3,674,083 + -0.8
Or see this region's nucleotide sequence