Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3806 and Shew_3807 are separated by 58 nucleotides Shew_3807 and Shew_3808 are separated by 35 nucleotides
Shew_3806: Shew_3806 - phosphoglyceromutase (RefSeq), at 4,528,912 to 4,530,456
_3806
Shew_3807: Shew_3807 - peptidase M23B (RefSeq), at 4,530,515 to 4,531,600
_3807
Shew_3808: Shew_3808 - carboxyl-terminal protease (RefSeq), at 4,531,636 to 4,532,841
_3808
Position (kb)
4530
4531
4532 Strain fitness (log2 ratio)
-1
0
1
2 at 4530.520 kb on + strand at 4530.520 kb on + strand at 4530.520 kb on + strand at 4530.520 kb on + strand at 4530.520 kb on + strand at 4530.520 kb on + strand at 4530.528 kb on - strand at 4530.528 kb on - strand at 4530.528 kb on - strand at 4530.528 kb on - strand at 4530.528 kb on - strand at 4531.053 kb on + strand, within Shew_3807 at 4531.080 kb on + strand, within Shew_3807 at 4531.088 kb on + strand, within Shew_3807 at 4531.297 kb on + strand, within Shew_3807 at 4531.328 kb on - strand, within Shew_3807 at 4531.428 kb on - strand, within Shew_3807 at 4531.476 kb on + strand, within Shew_3807 at 4531.492 kb on + strand at 4531.670 kb on + strand at 4531.678 kb on - strand at 4531.690 kb on - strand at 4531.777 kb on + strand, within Shew_3808 at 4531.851 kb on + strand, within Shew_3808 at 4531.959 kb on - strand, within Shew_3808 at 4532.008 kb on + strand, within Shew_3808 at 4532.160 kb on - strand, within Shew_3808 at 4532.174 kb on + strand, within Shew_3808 at 4532.174 kb on + strand, within Shew_3808 at 4532.174 kb on + strand, within Shew_3808 at 4532.182 kb on - strand, within Shew_3808 at 4532.182 kb on - strand, within Shew_3808 at 4532.182 kb on - strand, within Shew_3808 at 4532.203 kb on - strand, within Shew_3808 at 4532.276 kb on + strand, within Shew_3808 at 4532.458 kb on + strand, within Shew_3808 at 4532.535 kb on - strand, within Shew_3808
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 4,530,520 + +0.2 4,530,520 + -0.2 4,530,520 + -0.7 4,530,520 + -0.1 4,530,520 + -0.0 4,530,520 + +2.3 4,530,528 - -0.8 4,530,528 - +2.7 4,530,528 - -0.0 4,530,528 - +0.5 4,530,528 - +0.5 4,531,053 + Shew_3807 0.50 -0.5 4,531,080 + Shew_3807 0.52 +0.3 4,531,088 + Shew_3807 0.53 -1.0 4,531,297 + Shew_3807 0.72 -0.4 4,531,328 - Shew_3807 0.75 -0.2 4,531,428 - Shew_3807 0.84 -0.1 4,531,476 + Shew_3807 0.88 -0.1 4,531,492 + -0.2 4,531,670 + -0.1 4,531,678 - +0.7 4,531,690 - +0.1 4,531,777 + Shew_3808 0.12 -0.9 4,531,851 + Shew_3808 0.18 -0.1 4,531,959 - Shew_3808 0.27 +0.0 4,532,008 + Shew_3808 0.31 -0.1 4,532,160 - Shew_3808 0.43 +0.2 4,532,174 + Shew_3808 0.45 -0.4 4,532,174 + Shew_3808 0.45 +0.0 4,532,174 + Shew_3808 0.45 +0.5 4,532,182 - Shew_3808 0.45 +0.5 4,532,182 - Shew_3808 0.45 +0.6 4,532,182 - Shew_3808 0.45 -1.7 4,532,203 - Shew_3808 0.47 -0.0 4,532,276 + Shew_3808 0.53 -0.5 4,532,458 + Shew_3808 0.68 +0.1 4,532,535 - Shew_3808 0.75 -1.0
Or see this region's nucleotide sequence