Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2973 and Shew_2974 are separated by 200 nucleotides Shew_2974 and Shew_2975 are separated by 181 nucleotides Shew_2975 and Shew_2976 are separated by 14 nucleotides
Shew_2973: Shew_2973 - endothelin-converting protein 1 (RefSeq), at 3,526,276 to 3,528,360
_2973
Shew_2974: Shew_2974 - hexapaptide repeat-containing transferase (RefSeq), at 3,528,561 to 3,529,103
_2974
Shew_2975: Shew_2975 - pseudouridine synthase (RefSeq), at 3,529,285 to 3,529,977
_2975
Shew_2976: Shew_2976 - hypothetical protein (RefSeq), at 3,529,992 to 3,530,429
_2976
Position (kb)
3528
3529
3530 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3528.329 kb on + strand at 3528.940 kb on + strand, within Shew_2974 at 3529.053 kb on + strand at 3529.124 kb on - strand at 3529.137 kb on + strand at 3529.162 kb on + strand at 3529.173 kb on + strand at 3529.353 kb on - strand at 3529.515 kb on + strand, within Shew_2975 at 3529.698 kb on + strand, within Shew_2975 at 3529.706 kb on - strand, within Shew_2975 at 3529.756 kb on - strand, within Shew_2975 at 3529.961 kb on - strand at 3530.030 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 3,528,329 + +0.7 3,528,940 + Shew_2974 0.70 +3.3 3,529,053 + +1.0 3,529,124 - +0.2 3,529,137 + -0.3 3,529,162 + -0.5 3,529,173 + +1.7 3,529,353 - -0.2 3,529,515 + Shew_2975 0.33 -0.3 3,529,698 + Shew_2975 0.60 +0.2 3,529,706 - Shew_2975 0.61 +0.6 3,529,756 - Shew_2975 0.68 +2.0 3,529,961 - -0.0 3,530,030 - -0.4
Or see this region's nucleotide sequence