Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2098 and Shew_2099 overlap by 27 nucleotides Shew_2099 and Shew_2100 are separated by 154 nucleotides Shew_2100 and Shew_2101 overlap by 1 nucleotides
Shew_2098: Shew_2098 - hemolysin III family channel protein (RefSeq), at 2,426,803 to 2,427,513
_2098
Shew_2099: Shew_2099 - DTW domain-containing protein (RefSeq), at 2,427,487 to 2,428,056
_2099
Shew_2100: Shew_2100 - DNA polymerase III, alpha subunit (RefSeq), at 2,428,211 to 2,431,447
_2100
Shew_2101: Shew_2101 - hypothetical protein (RefSeq), at 2,431,447 to 2,433,501
_2101
Position (kb)
2428
2429
2430
2431
2432 Strain fitness (log2 ratio)
-1
0
1 at 2427.231 kb on + strand, within Shew_2098 at 2427.663 kb on - strand, within Shew_2099 at 2427.944 kb on + strand, within Shew_2099 at 2427.954 kb on + strand, within Shew_2099 at 2428.137 kb on - strand at 2428.645 kb on + strand, within Shew_2100 at 2428.786 kb on + strand, within Shew_2100 at 2428.862 kb on - strand, within Shew_2100 at 2428.928 kb on - strand, within Shew_2100 at 2429.071 kb on - strand, within Shew_2100 at 2429.104 kb on - strand, within Shew_2100 at 2429.384 kb on - strand, within Shew_2100 at 2429.527 kb on + strand, within Shew_2100 at 2430.059 kb on - strand, within Shew_2100 at 2430.559 kb on - strand, within Shew_2100 at 2430.649 kb on - strand, within Shew_2100 at 2430.673 kb on - strand, within Shew_2100 at 2431.135 kb on - strand at 2431.912 kb on + strand, within Shew_2101 at 2432.021 kb on - strand, within Shew_2101 at 2432.136 kb on + strand, within Shew_2101
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 2,427,231 + Shew_2098 0.60 +0.5 2,427,663 - Shew_2099 0.31 -0.3 2,427,944 + Shew_2099 0.80 -1.7 2,427,954 + Shew_2099 0.82 +0.3 2,428,137 - +0.2 2,428,645 + Shew_2100 0.13 -0.1 2,428,786 + Shew_2100 0.18 -1.4 2,428,862 - Shew_2100 0.20 -0.8 2,428,928 - Shew_2100 0.22 -1.4 2,429,071 - Shew_2100 0.27 -1.2 2,429,104 - Shew_2100 0.28 -0.6 2,429,384 - Shew_2100 0.36 -0.9 2,429,527 + Shew_2100 0.41 -0.5 2,430,059 - Shew_2100 0.57 -0.3 2,430,559 - Shew_2100 0.73 +1.2 2,430,649 - Shew_2100 0.75 -0.0 2,430,673 - Shew_2100 0.76 +0.4 2,431,135 - -1.1 2,431,912 + Shew_2101 0.23 +0.3 2,432,021 - Shew_2101 0.28 -1.5 2,432,136 + Shew_2101 0.34 -0.6
Or see this region's nucleotide sequence