Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2062 and Shew_2063 overlap by 7 nucleotides Shew_2063 and Shew_2064 are separated by 126 nucleotides Shew_2064 and Shew_R0074 are separated by 744 nucleotides Shew_R0074 and Shew_R0075 are separated by 42 nucleotides Shew_R0075 and Shew_R0076 are separated by 26 nucleotides
Shew_2062: Shew_2062 - exsB protein (RefSeq), at 2,381,452 to 2,382,135
_2062
Shew_2063: Shew_2063 - rhomboid family protein (RefSeq), at 2,382,129 to 2,382,713
_2063
Shew_2064: Shew_2064 - excinuclease ABC subunit B (RefSeq), at 2,382,840 to 2,384,858
_2064
Shew_R0074: Shew_R0074 - tRNA-Asn (RefSeq), at 2,385,603 to 2,385,678
_R0074
Shew_R0075: Shew_R0075 - tRNA-Asn (RefSeq), at 2,385,721 to 2,385,796
_R0075
Shew_R0076: Shew_R0076 - tRNA-Asn (RefSeq), at 2,385,823 to 2,385,898
_R0076
Position (kb)
2382
2383
2384
2385 Strain fitness (log2 ratio)
-1
0
1 at 2381.978 kb on + strand, within Shew_2062 at 2382.216 kb on - strand, within Shew_2063 at 2382.559 kb on + strand, within Shew_2063 at 2383.002 kb on - strand at 2383.212 kb on + strand, within Shew_2064 at 2383.341 kb on - strand, within Shew_2064 at 2383.806 kb on - strand, within Shew_2064 at 2384.126 kb on + strand, within Shew_2064 at 2384.281 kb on - strand, within Shew_2064 at 2384.868 kb on + strand at 2384.876 kb on - strand at 2385.063 kb on - strand at 2385.307 kb on + strand at 2385.371 kb on + strand at 2385.393 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 2,381,978 + Shew_2062 0.77 +0.0 2,382,216 - Shew_2063 0.15 -1.3 2,382,559 + Shew_2063 0.74 -1.3 2,383,002 - +0.4 2,383,212 + Shew_2064 0.18 -0.5 2,383,341 - Shew_2064 0.25 -0.3 2,383,806 - Shew_2064 0.48 -1.0 2,384,126 + Shew_2064 0.64 -0.7 2,384,281 - Shew_2064 0.71 -1.3 2,384,868 + -0.4 2,384,876 - -1.1 2,385,063 - +0.5 2,385,307 + -0.7 2,385,371 + +0.3 2,385,393 + -0.1
Or see this region's nucleotide sequence