Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1477 and Shew_1478 are separated by 49 nucleotides Shew_1478 and Shew_1479 overlap by 4 nucleotides Shew_1479 and Shew_1480 are separated by 1 nucleotides Shew_1480 and Shew_1481 are separated by 9 nucleotides
Shew_1477: Shew_1477 - anti-ECFsigma factor, ChrR (RefSeq), at 1,702,337 to 1,703,086
_1477
Shew_1478: Shew_1478 - hypothetical protein (RefSeq), at 1,703,136 to 1,704,080
_1478
Shew_1479: Shew_1479 - deoxyribodipyrimidine photo-lyase (RefSeq), at 1,704,077 to 1,705,513
_1479
Shew_1480: Shew_1480 - hypothetical protein (RefSeq), at 1,705,515 to 1,705,958
_1480
Shew_1481: Shew_1481 - short-chain dehydrogenase/reductase SDR (RefSeq), at 1,705,968 to 1,706,690
_1481
Position (kb)
1704
1705
1706 Strain fitness (log2 ratio)
-2
-1
0
1 at 1703.331 kb on + strand, within Shew_1478 at 1703.345 kb on - strand, within Shew_1478 at 1705.064 kb on - strand, within Shew_1479 at 1705.201 kb on - strand, within Shew_1479 at 1705.449 kb on - strand at 1705.473 kb on - strand at 1705.560 kb on + strand, within Shew_1480 at 1705.577 kb on + strand, within Shew_1480 at 1705.638 kb on + strand, within Shew_1480 at 1705.780 kb on + strand, within Shew_1480 at 1705.790 kb on + strand, within Shew_1480 at 1705.798 kb on - strand, within Shew_1480 at 1705.935 kb on + strand at 1705.943 kb on - strand at 1706.105 kb on + strand, within Shew_1481 at 1706.342 kb on - strand, within Shew_1481
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 1,703,331 + Shew_1478 0.21 +0.3 1,703,345 - Shew_1478 0.22 +0.0 1,705,064 - Shew_1479 0.69 -1.2 1,705,201 - Shew_1479 0.78 -1.2 1,705,449 - -0.5 1,705,473 - +1.2 1,705,560 + Shew_1480 0.10 -0.0 1,705,577 + Shew_1480 0.14 -1.8 1,705,638 + Shew_1480 0.28 -0.0 1,705,780 + Shew_1480 0.60 -0.5 1,705,790 + Shew_1480 0.62 +1.0 1,705,798 - Shew_1480 0.64 -0.2 1,705,935 + +0.1 1,705,943 - +0.2 1,706,105 + Shew_1481 0.19 +0.0 1,706,342 - Shew_1481 0.52 +0.6
Or see this region's nucleotide sequence