Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhF and Shew_1382 overlap by 26 nucleotides Shew_1382 and fliA overlap by 8 nucleotides fliA and Shew_1384 are separated by 42 nucleotides Shew_1384 and Shew_1385 are separated by 13 nucleotides
Shew_1381: flhF - flagellar biosynthesis regulator FlhF (RefSeq), at 1,590,430 to 1,591,854
flhF
Shew_1382: Shew_1382 - cobyrinic acid a,c-diamide synthase (RefSeq), at 1,591,829 to 1,592,740
_1382
Shew_1383: fliA - flagellar biosynthesis sigma factor (RefSeq), at 1,592,733 to 1,593,452
fliA
Shew_1384: Shew_1384 - response regulator receiver protein (RefSeq), at 1,593,495 to 1,593,878
_1384
Shew_1385: Shew_1385 - chemotaxis phosphatase, CheZ (RefSeq), at 1,593,892 to 1,594,629
_1385
Position (kb)
1592
1593
1594 Strain fitness (log2 ratio)
-1
0
1 at 1591.736 kb on + strand at 1592.030 kb on + strand, within Shew_1382 at 1592.144 kb on - strand, within Shew_1382 at 1592.213 kb on + strand, within Shew_1382 at 1592.872 kb on - strand, within fliA at 1592.874 kb on + strand, within fliA at 1593.035 kb on - strand, within fliA at 1593.695 kb on + strand, within Shew_1384 at 1593.905 kb on + strand at 1593.930 kb on + strand at 1594.241 kb on - strand, within Shew_1385 at 1594.277 kb on + strand, within Shew_1385 at 1594.323 kb on - strand, within Shew_1385 at 1594.410 kb on - strand, within Shew_1385 at 1594.450 kb on + strand, within Shew_1385
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 1,591,736 + -1.1 1,592,030 + Shew_1382 0.22 -0.1 1,592,144 - Shew_1382 0.35 +1.1 1,592,213 + Shew_1382 0.42 +0.1 1,592,872 - fliA Shew_1383 0.19 -0.9 1,592,874 + fliA Shew_1383 0.20 -0.9 1,593,035 - fliA Shew_1383 0.42 -0.9 1,593,695 + Shew_1384 0.52 -0.2 1,593,905 + +0.2 1,593,930 + -1.3 1,594,241 - Shew_1385 0.47 +0.1 1,594,277 + Shew_1385 0.52 +0.0 1,594,323 - Shew_1385 0.58 -0.0 1,594,410 - Shew_1385 0.70 +0.4 1,594,450 + Shew_1385 0.76 +0.2
Or see this region's nucleotide sequence