Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0858 and Shew_0859 are separated by 10 nucleotides Shew_0859 and Shew_0860 are separated by 78 nucleotides Shew_0860 and Shew_0861 are separated by 169 nucleotides
Shew_0858: Shew_0858 - oxidoreductase FAD/NAD(P)-binding subunit (RefSeq), at 979,680 to 980,420
_0858
Shew_0859: Shew_0859 - glutathione S-transferase domain-containing protein (RefSeq), at 980,431 to 981,030
_0859
Shew_0860: Shew_0860 - hypothetical protein (RefSeq), at 981,109 to 981,579
_0860
Shew_0861: Shew_0861 - extracellular solute-binding protein (RefSeq), at 981,749 to 982,756
_0861
Position (kb) 981
982 Strain fitness (log2 ratio) -2
-1
0
1 at 980.452 kb on + strand at 980.463 kb on + strand at 980.463 kb on + strand at 980.471 kb on - strand at 980.712 kb on - strand, within Shew_0859 at 980.712 kb on - strand, within Shew_0859 at 980.758 kb on + strand, within Shew_0859 at 980.815 kb on - strand, within Shew_0859 at 981.125 kb on + strand at 981.393 kb on - strand, within Shew_0860 at 981.393 kb on - strand, within Shew_0860 at 981.925 kb on - strand, within Shew_0861 at 982.047 kb on + strand, within Shew_0861 at 982.060 kb on - strand, within Shew_0861 at 982.131 kb on + strand, within Shew_0861 at 982.131 kb on + strand, within Shew_0861 at 982.435 kb on + strand, within Shew_0861 at 982.443 kb on - strand, within Shew_0861
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 980,452 + +0.1 980,463 + -0.2 980,463 + +1.0 980,471 - +0.0 980,712 - Shew_0859 0.47 -0.0 980,712 - Shew_0859 0.47 +0.2 980,758 + Shew_0859 0.55 -0.6 980,815 - Shew_0859 0.64 -0.5 981,125 + +0.5 981,393 - Shew_0860 0.60 -2.7 981,393 - Shew_0860 0.60 -0.3 981,925 - Shew_0861 0.17 +0.2 982,047 + Shew_0861 0.30 +0.4 982,060 - Shew_0861 0.31 -0.7 982,131 + Shew_0861 0.38 -0.1 982,131 + Shew_0861 0.38 -0.3 982,435 + Shew_0861 0.68 -0.2 982,443 - Shew_0861 0.69 -0.2
Or see this region's nucleotide sequence