Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0645 and Shew_0646 overlap by 4 nucleotides Shew_0646 and Shew_0647 are separated by 14 nucleotides Shew_0647 and Shew_0648 are separated by 7 nucleotides Shew_0648 and Shew_0649 are separated by 4 nucleotides
Shew_0645: Shew_0645 - type II and III secretion system protein (RefSeq), at 733,888 to 735,198
_0645
Shew_0646: Shew_0646 - SAF domain-containing protein (RefSeq), at 735,195 to 736,091
_0646
Shew_0647: Shew_0647 - hypothetical protein (RefSeq), at 736,106 to 736,297
_0647
Shew_0648: Shew_0648 - hypothetical protein (RefSeq), at 736,305 to 736,976
_0648
Shew_0649: Shew_0649 - hypothetical protein (RefSeq), at 736,981 to 737,841
_0649
Position (kb) 735
736
737 Strain fitness (log2 ratio) -1
0
1 at 734.690 kb on + strand, within Shew_0645 at 734.931 kb on + strand, within Shew_0645 at 735.233 kb on - strand at 735.270 kb on + strand at 735.539 kb on + strand, within Shew_0646 at 735.875 kb on - strand, within Shew_0646 at 735.875 kb on - strand, within Shew_0646 at 735.952 kb on + strand, within Shew_0646 at 736.189 kb on - strand, within Shew_0647 at 736.217 kb on - strand, within Shew_0647 at 736.292 kb on + strand at 736.439 kb on + strand, within Shew_0648 at 736.586 kb on + strand, within Shew_0648 at 736.836 kb on - strand, within Shew_0648 at 736.936 kb on - strand at 736.991 kb on - strand at 737.069 kb on + strand, within Shew_0649
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 734,690 + Shew_0645 0.61 -1.2 734,931 + Shew_0645 0.80 -0.1 735,233 - +0.1 735,270 + -0.8 735,539 + Shew_0646 0.38 -0.4 735,875 - Shew_0646 0.76 -0.9 735,875 - Shew_0646 0.76 -0.4 735,952 + Shew_0646 0.84 -0.4 736,189 - Shew_0647 0.43 +0.0 736,217 - Shew_0647 0.58 -0.5 736,292 + +0.5 736,439 + Shew_0648 0.20 -0.2 736,586 + Shew_0648 0.42 +0.2 736,836 - Shew_0648 0.79 -0.1 736,936 - +0.0 736,991 - -1.0 737,069 + Shew_0649 0.10 -0.1
Or see this region's nucleotide sequence