Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0606 and Shew_0607 overlap by 4 nucleotides Shew_0607 and Shew_0608 are separated by 12 nucleotides Shew_0608 and Shew_0609 are separated by 290 nucleotides
Shew_0606: Shew_0606 - polysulphide reductase, NrfD (RefSeq), at 684,935 to 685,876
_0606
Shew_0607: Shew_0607 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 685,873 to 686,445
_0607
Shew_0608: Shew_0608 - molybdopterin oxidoreductase (RefSeq), at 686,458 to 688,740
_0608
Shew_0609: Shew_0609 - two component LuxR family transcriptional regulator (RefSeq), at 689,031 to 689,630
_0609
Position (kb) 686
687
688
689 Strain fitness (log2 ratio) -1
0
1 at 685.657 kb on + strand, within Shew_0606 at 685.675 kb on - strand, within Shew_0606 at 685.697 kb on + strand, within Shew_0606 at 685.782 kb on - strand at 685.914 kb on + strand at 685.922 kb on - strand at 685.922 kb on - strand at 685.937 kb on + strand, within Shew_0607 at 686.846 kb on - strand, within Shew_0608 at 686.898 kb on + strand, within Shew_0608 at 687.101 kb on + strand, within Shew_0608 at 687.150 kb on - strand, within Shew_0608 at 687.337 kb on + strand, within Shew_0608 at 687.560 kb on + strand, within Shew_0608 at 687.651 kb on + strand, within Shew_0608 at 687.659 kb on - strand, within Shew_0608 at 687.724 kb on - strand, within Shew_0608 at 687.868 kb on - strand, within Shew_0608 at 687.868 kb on - strand, within Shew_0608 at 687.975 kb on - strand, within Shew_0608 at 688.335 kb on + strand, within Shew_0608 at 688.449 kb on + strand, within Shew_0608 at 688.465 kb on + strand, within Shew_0608 at 688.470 kb on + strand, within Shew_0608 at 688.624 kb on + strand at 688.779 kb on - strand at 688.869 kb on + strand at 689.090 kb on + strand at 689.198 kb on + strand, within Shew_0609 at 689.392 kb on - strand, within Shew_0609 at 689.549 kb on + strand, within Shew_0609 at 689.557 kb on - strand, within Shew_0609 at 689.679 kb on + strand at 689.687 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 685,657 + Shew_0606 0.77 -0.3 685,675 - Shew_0606 0.79 +1.0 685,697 + Shew_0606 0.81 +0.9 685,782 - +1.4 685,914 + +0.1 685,922 - +1.1 685,922 - -0.4 685,937 + Shew_0607 0.11 +1.5 686,846 - Shew_0608 0.17 -0.6 686,898 + Shew_0608 0.19 -0.4 687,101 + Shew_0608 0.28 +0.0 687,150 - Shew_0608 0.30 +0.3 687,337 + Shew_0608 0.39 -0.5 687,560 + Shew_0608 0.48 +0.3 687,651 + Shew_0608 0.52 -0.4 687,659 - Shew_0608 0.53 -0.1 687,724 - Shew_0608 0.55 +0.9 687,868 - Shew_0608 0.62 -1.0 687,868 - Shew_0608 0.62 +0.1 687,975 - Shew_0608 0.66 -0.2 688,335 + Shew_0608 0.82 -0.2 688,449 + Shew_0608 0.87 -0.5 688,465 + Shew_0608 0.88 +0.2 688,470 + Shew_0608 0.88 +0.1 688,624 + -0.1 688,779 - -0.3 688,869 + +0.1 689,090 + +0.2 689,198 + Shew_0609 0.28 +0.1 689,392 - Shew_0609 0.60 -0.5 689,549 + Shew_0609 0.86 +0.3 689,557 - Shew_0609 0.88 -0.3 689,679 + -0.6 689,687 - -0.0
Or see this region's nucleotide sequence