Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0565 and Shew_0566 are separated by 92 nucleotides Shew_0566 and Shew_0567 are separated by 20 nucleotides Shew_0567 and Shew_0568 are separated by 43 nucleotides
Shew_0565: Shew_0565 - tRNA delta(2)-isopentenylpyrophosphate transferase (RefSeq), at 641,153 to 642,079
_0565
Shew_0566: Shew_0566 - RNA chaperone Hfq (RefSeq), at 642,172 to 642,450
_0566
Shew_0567: Shew_0567 - GTP-binding protein, HSR1-related (RefSeq), at 642,471 to 643,769
_0567
Shew_0568: Shew_0568 - HflK protein (RefSeq), at 643,813 to 644,955
_0568
Position (kb) 642
643
644 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 641.590 kb on + strand, within Shew_0565 at 641.717 kb on + strand, within Shew_0565 at 641.768 kb on + strand, within Shew_0565 at 641.768 kb on + strand, within Shew_0565 at 641.987 kb on + strand at 642.371 kb on + strand, within Shew_0566 at 642.499 kb on + strand at 642.504 kb on + strand at 642.538 kb on + strand at 642.839 kb on + strand, within Shew_0567 at 643.324 kb on + strand, within Shew_0567 at 643.798 kb on + strand at 643.981 kb on + strand, within Shew_0568 at 644.378 kb on + strand, within Shew_0568 at 644.606 kb on + strand, within Shew_0568 at 644.606 kb on + strand, within Shew_0568
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 641,590 + Shew_0565 0.47 +1.9 641,717 + Shew_0565 0.61 +0.0 641,768 + Shew_0565 0.66 -0.5 641,768 + Shew_0565 0.66 -2.3 641,987 + +0.3 642,371 + Shew_0566 0.71 -3.3 642,499 + -1.0 642,504 + -1.1 642,538 + -3.0 642,839 + Shew_0567 0.28 -2.1 643,324 + Shew_0567 0.66 -0.6 643,798 + +1.7 643,981 + Shew_0568 0.15 +0.4 644,378 + Shew_0568 0.49 +0.1 644,606 + Shew_0568 0.69 +0.0 644,606 + Shew_0568 0.69 +0.2
Or see this region's nucleotide sequence