Experiment: Doxycycline hyclate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0564 and Shew_0565 overlap by 8 nucleotides Shew_0565 and Shew_0566 are separated by 92 nucleotides Shew_0566 and Shew_0567 are separated by 20 nucleotides
Shew_0564: Shew_0564 - DNA mismatch repair protein MutL (RefSeq), at 639,265 to 641,160
_0564
Shew_0565: Shew_0565 - tRNA delta(2)-isopentenylpyrophosphate transferase (RefSeq), at 641,153 to 642,079
_0565
Shew_0566: Shew_0566 - RNA chaperone Hfq (RefSeq), at 642,172 to 642,450
_0566
Shew_0567: Shew_0567 - GTP-binding protein, HSR1-related (RefSeq), at 642,471 to 643,769
_0567
Position (kb) 641
642
643 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 640.178 kb on + strand, within Shew_0564 at 640.186 kb on - strand, within Shew_0564 at 640.225 kb on - strand, within Shew_0564 at 640.519 kb on + strand, within Shew_0564 at 640.586 kb on - strand, within Shew_0564 at 640.643 kb on + strand, within Shew_0564 at 640.699 kb on + strand, within Shew_0564 at 641.242 kb on + strand at 641.590 kb on + strand, within Shew_0565 at 641.717 kb on + strand, within Shew_0565 at 641.768 kb on + strand, within Shew_0565 at 641.768 kb on + strand, within Shew_0565 at 641.987 kb on + strand at 642.371 kb on + strand, within Shew_0566 at 642.499 kb on + strand at 642.504 kb on + strand at 642.538 kb on + strand at 642.839 kb on + strand, within Shew_0567
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 10 mg/ml remove 640,178 + Shew_0564 0.48 +0.6 640,186 - Shew_0564 0.49 -0.9 640,225 - Shew_0564 0.51 -0.0 640,519 + Shew_0564 0.66 +0.1 640,586 - Shew_0564 0.70 -0.3 640,643 + Shew_0564 0.73 +0.2 640,699 + Shew_0564 0.76 +0.4 641,242 + -2.2 641,590 + Shew_0565 0.47 +1.9 641,717 + Shew_0565 0.61 +0.0 641,768 + Shew_0565 0.66 -0.5 641,768 + Shew_0565 0.66 -2.3 641,987 + +0.3 642,371 + Shew_0566 0.71 -3.3 642,499 + -1.0 642,504 + -1.1 642,538 + -3.0 642,839 + Shew_0567 0.28 -2.1
Or see this region's nucleotide sequence